Positive control ChIP-qPCR 5' and 3' primers for RPL30 gene.
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Key facts
- Applications
- ChIP
- Form
- Lyophilized
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ChIP | Reactivity Reacts | Dilution info - | Notes - |
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Positive control ChIP-qPCR 5' and 3' primers for RPL30 gene.
Key facts
- Applications
- ChIP
- Form
- Lyophilized
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Positive control ChIP-qPCR 5' and 3' primers for RPL30 gene. Use with SYBR green.
We recommend these primers as a positive control (based on Abcam's testing) for the histone marks below. They may also be useful for other histone marks.
Suitable positive control for:
- Histone H3 di methyl K36
- Histone H3 acetyl K23
- Histone H3 phospho S10
500pmole of each oligo per unit (lyophilised). HPLC purified, desalted and lyophilised as a sodium salt.
Quantity provided is sufficient for approx. 200 reactions based on 2.5pmol of primer per reaction with a final concentration of 100nM in 25μl.
Please contact us after purchase if you require the sequence of the oligos.
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3 product images
ChIP - RPL30 positive control ChIP primer pair (ab269262)
Chromatin was prepared from HeLa (Human epithelial cells from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. HeLa (Human epithelial cells from cervix adenocarcinoma) cells were treated with colcemid at 150ng/ml for 20 h. Treated and non-treated cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of Anti-Histone H3 (phospho S10) antibody [EPR17246] - ChIP Grade ab177218 (blue), and 20μl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
ChIP - RPL30 positive control ChIP primer pair (ab269262)
Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of Anti-Histone H3 (di methyl K36) antibody [EPR16994(2)] - ChIP Grade ab176921 (red), and 20μl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
ChIP - RPL30 positive control ChIP primer pair (ab269262)
Chromatin was prepared from HeLa (Human epithelial cells from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of Anti-Histone H3 (acetyl K23) antibody [EPR17712] - ChIP Grade ab177275 (blue), and 20μl of Anti Rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
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