Positive control ChIP-qPCR 5' and 3' primers for SAT-alpha.
Images
Key facts
- Applications
- ChIP
- Form
- Lyophilized
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ChIP | Reactivity Reacts | Dilution info - | Notes - |
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Positive control ChIP-qPCR 5' and 3' primers for SAT-alpha.
Key facts
- Applications
- ChIP
- Form
- Lyophilized
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Positive control ChIP-qPCR 5' and 3' primers for SAT-alpha. Use with SYBR green.
We recommend these primers as a positive control (based on Abcam's testing) for the histone marks below. They may also be useful for other histone marks.
Suitable positive control for:
- Histone H3 tri methyl K9
- Histone H3 phospho S28
500pmole of each oligo per unit (lyophilised). HPLC purified, desalted and lyophilised as a sodium salt.
Quantity provided is sufficient for approx. 200 reactions based on 2.5pmol of primer per reaction with a final concentration of 100nM in 25μl.
Please contact us after purchase if you require the sequence of the oligos.
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2 product images
ChIP - SAT-alpha positive control ChIP primer pair (ab269263)
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade ab176916 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
ChIP - SAT-alpha positive control ChIP primer pair (ab269263)
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 5 μg of Anti-Histone H3 (phospho S28) antibody [E191] - ChIP Grade ab32388 (red), and 20 μl of Protein A/G sepharose beads. Rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
Primers and probes are located in the first kb of the transcribed region.
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