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AB210902

TMB substrate 1X

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(4 Publications)

TMB substrate 1X is recommended to use with our Matched Antibody Pair kits for sandwich ELISA development.

- Detects horseradish peroxidase activity (HRP)
- Yields a blue color (620 nm) that changes to yellow (450 nm) upon addition of a Stop Solution

Key facts

Form

Liquid

form

Storage buffer

Constituents: 0.2% Proprietary Non-haz Component, 0.05% 3,3',5,5'-Tetramethylbenzidine, 0.02% Hydrogen peroxide

storage-buffer

Product details

TMB substrate is recommended for use with the Matched Antibody Pair kits for sandwich ELISA. TMB Substrate detects horseradish peroxidase activity, yielding a blue color (620 nm) that changes to yellow (450 nm) upon addition of a Stop Solution. The TMB substrate is supplied ready to use. See our for a protocol.The TMB substrate is also avaialble as part of our Matched Antibody Pair Kit Accessory Pack (ab210905). Product should be stored at 2-8°C. Exposure to direct sunlight and other UV sources should be avoided due to the light sensitive nature of the TMB molecule.

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Product protocols

Target data

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5728 PubMed40593621

2025

HDL-bound S1P affects the subventricular niche and early neuropathological features of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Byung Jo Choi,Ju Yeon Hong,Min Hee Park,Kang Ho Park,Wan Hui Han,Hee Ji Yoon,Hye Yoon Jung,Kyung Yeol Kim,Sun Ae Lee,Eun Young Lim,Jung Woo Hur,Im-Sook Song,So Yeon Jeon,Min-Koo Choi,Christina Christoffersen,Hee-Jin Kim,Seung Hyun Kim,Edward H Schuchman,Jae-Sung Bae,Hee Kyung Jin

Nature communications 14:1631 PubMed36959217

2023

Immunotherapy targeting plasma ASM is protective in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Byung Jo Choi,Min Hee Park,Kang Ho Park,Wan Hui Han,Hee Ji Yoon,Hye Yoon Jung,Ju Yeon Hong,Md Riad Chowdhury,Kyung Yeol Kim,Jihoon Lee,Im-Sook Song,Minyeong Pang,Min-Koo Choi,Erich Gulbins,Martin Reichel,Johannes Kornhuber,Chang-Won Hong,Changho Kim,Seung Hyun Kim,Edward H Schuchman,Hee Kyung Jin,Jae-Sung Bae

Cancers 13: PubMed34572924

2021

Cathepsin S Evokes PAR-Dependent Pain in Oral Squamous Cell Carcinoma Patients and Preclinical Mouse Models.

Applications

Unspecified application

Species

Unspecified reactive species

Nguyen Huu Tu,Kenji Inoue,Elyssa Chen,Bethany M Anderson,Caroline M Sawicki,Nicole N Scheff,Hung D Tran,Dong H Kim,Robel G Alemu,Lei Yang,John C Dolan,Cheng Z Liu,Malvin N Janal,Rocco Latorre,Dane D Jensen,Nigel W Bunnett,Laura E Edgington-Mitchell,Brian L Schmidt

International journal of molecular sciences 21: PubMed33218072

2020

Discovery and Design of Novel Small Molecule GSK-3 Inhibitors Targeting the Substrate Binding Site.

Applications

Unspecified application

Species

Unspecified reactive species

Ido Rippin,Netaly Khazanov, Shirley Ben Joseph,Tania Kudinov,Eva Berent,Sara Melisa Arciniegas Ruiz,Daniele Marciano,Laura Levy,Arie Gruzman,Hanoch Senderowitz,Hagit Eldar-Finkelman
View all publications

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