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Trypsin-based antigen retrieval solution for Protease-Induced Epitope Retrieval (PIER) (ab970).


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Key facts

Applications

IHC-P

Form

Liquid

Reactivity data

Application

IHC-P

Reactivity

Reacts

Dilution info

-

Notes

Ratio for 25ml concentrated trypsin and 25 ml buffer is 1:1 - 1:3.

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Trypsin-based antigen retrieval solution for Protease-Induced Epitope Retrieval (PIER) (ab970).

Key facts

Applications

IHC-P

Form

Liquid

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: Carrier protein, PBS

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

Trypsin-based antigen retrieval solution for Protease-Induced Epitope Retrieval (PIER) (ab970). Contains colored pH indicator for easy pH confirmation.

Protocol Notes: 1) Deparaffinize tissues and hydrate to water. If necessary perform a hydrogen peroxide block, wash in water, and rinse in PBS or TBS. The tissue section is ready for trypsin digestion. 2) Combine 1 part trypsin concentrate with 1 part buffer (working solution stable for 5 working days at 2-8°C). Incubate section for 5-10 minutes at 37°C or 15 minutes at room temperature. Then, rinse well in PBS or TBS buffer and proceed with immunostaining procedure. This product utilises a color-coded pH indicator (rose) for easy identification. The end-user can visually inspect the solution and see that the mixture or buffer is at the proper pH. If the mixed solution is purple, the pH is too high for optimum digestion. If the buffer or trypsin solution turns orange or yellow, the pH is too low for optimum digestion.

Trypsin is a commonly used digestive enzyme. In formalin-fixed, paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining.

Other products for IHC

Other enzymes for antigen retrieval include: Proteinase K Antigen Retrieval Solution ab64220 and Pepsin Solution ab64201.

Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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