VeriBlot for IP Detection Reagent (HRP)

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SLAMF6 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab224201 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224201 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] (<a href='/en-us/products/primary-antibodies/slamf6-antibody-epr22170-ab224201'>ab224201</a>) at 1/500 dilution

Lane 1:

Jurkat whole cell lysate (input) at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/slamf6-antibody-epr22170-ab224201'>ab224201</a> at 1/30 IP in Jurkat whole cell lysate (+) at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/slamf6-antibody-epr22170-ab224201'>ab224201</a> in Jurkat whole cell lysate (-) at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 37 kDa

true

Exposure time: 10s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

HMGCS2 was immunoprecipitated from 0.35 mg Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate 10 μg with ab305227 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305227 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate 10 μg Lane 2 : ab305227 IP in Caco-2 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305227 in Caco-2 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-HMGCS2 antibody [EPR26978-39] (<a href='/en-us/products/primary-antibodies/hmgcs2-antibody-epr26978-39-ab305227'>ab305227</a>) at 1/30 dilution

All lanes:

Caco-2 (human colorectal adenocarcinoma epithelial cell)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 50 kDa

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

All lanes:

Immunoprecipitation - Anti-ALDH7A1 antibody [EP1935Y] (<a href='/en-us/products/primary-antibodies/aldh7a1-antibody-ep1935y-ab53278'>ab53278</a>) at 1/20 dilution

false

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

PBRM1/BAF180 was immunoprecipitated from 0.35 mg HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate 10 µg with ab305223 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305223 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate 10 µg Lane 2 : ab305223 IP in HAP1 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305223 in HAP1 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 102 seconds

All lanes:

Immunoprecipitation - Anti-PBRM1/BAF180 antibody [EPR25199-139] (<a href='/en-us/products/primary-antibodies/pbrm1-baf180-antibody-epr25199-139-ab305223'>ab305223</a>) at 1/30 dilution

All lanes:

HAP1 (human chronic myelogenous leukemia near-haploid cell)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 205 kDa

false

Exposure time: 102s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SIGLEC8 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) cells transfected with a human SIGLEC8 expression vector containing a myc-His-tag®, whole cell lysate, 2 μg with ab305297 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305297 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HEK-293T (human embryonic kidney epithelial cell) cells transfected with a human SIGLEC8 expression vector containing a myc-His-tag®, whole cell lysate, 2 μg Lane 2 : ab305297 IP in HEK-293T cells transfected with a human SIGLEC8 expression vector containing a myc-His-tag®, whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305297 in HEK-293T cells transfected with a human SIGLEC8 expression vector containing a myc-His-tag®, whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 seconds

All lanes:

Immunoprecipitation - Anti-SIGLEC8 antibody [EPR24419-56] (<a href='/en-us/products/primary-antibodies/siglec8-antibody-epr24419-56-ab305297'>ab305297</a>) at 1/30 dilution

All lanes:

HEK-293T (human embryonic kidney epithelial cell)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 60 kDa

false

Exposure time: 3s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Synaptogyrin 1 was immunoprecipitated from 0.35 mg Human hypothalamus tissue lysate with ab308617 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308617 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Human hypothalamus tissue lysate Lane 2 : abAB308617 IP in Human hypothalamus tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab308617 in human hypothalamus tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 1 second

All lanes:

Immunoprecipitation - Anti-Synaptogyrin 1 antibody [EPR25081-18] (<a href='/en-us/products/primary-antibodies/synaptogyrin-1-antibody-epr25081-18-ab308617'>ab308617</a>) at 1/30 dilution

All lanes:

Human hypothalamus tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 26 kDa

false

Exposure time: 1s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

ARID1A was immunoprecipitated from 0.35 mg Wild-type HAP1 treated with 10 μM MG-132 for 6 hours whole cell lysate 10 μg with ab305172 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305172 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Wild-type HAP1 treated with 10 μM MG-132 for 6 hours whole cell lysate 10 μg Lane 2 : ab305172 IP Wild-type HAP1 treated with 10 μM MG-132 for 6 hours whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305172 in Wild-type HAP1 treated with 10 μM MG-132 for 6 hours whole cell lysate Lane 4 : ARID1A knockout HAP1 treated with 10 μM MG-132 for 6 hours whole cell lysate 10 μg Lane 5 : ARID1A knockout HAP1 treated with 10 μM MG-132 for 6 hours whole cell lysate Lane 6 : Rabbit monoclonal IgG (ab172730) instead of ab305172 in ARID1A knockout HAP1 treated with 10 μM MG-132 for 6 hours whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-ARID1A antibody [EPR25407-17] (<a href='/en-us/products/primary-antibodies/arid1a-antibody-epr25407-17-ab305172'>ab305172</a>) at 1/30 dilution

All lanes:

Wild-type HAP1

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 250 kDa

false

Exposure time: 3min

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

AHA1 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 μg with ab307716 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307716 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 μg Lane 2 : ab307716 IP in HeLa whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of 307716 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds

All lanes:

Immunoprecipitation - Anti-AHA1 antibody [EPR26852-47] (<a href='/en-us/products/primary-antibodies/aha1-antibody-epr26852-47-ab307716'>ab307716</a>) at 1/30 dilution

All lanes:

HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 42 kDa

false

Exposure time: 15s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

HIF-1 alpha was immunoprecipitated from 0.35 mg HCT116 (human colorectal carcinoma epithelial cell) treated with 0.5 mM DFO for 24 hour whole cell lysate with ab308433 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308433 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HCT116 (human colorectal carcinoma epithelial cell) treated with 0.5 mM DFO for 24 hour whole cell lysate Lane 2 : abAB308433 IP in HCT116 (human colorectal carcinoma epithelial cell) treated with 0.5 mM DFO for 24 hour whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab308433 in HCT116 treated with 0.5 mM DFO for 24 hour whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds

All lanes:

Immunoprecipitation - Anti-HIF-1 alpha antibody [EPR16897-145] (<a href='/en-us/products/primary-antibodies/hif-1-alpha-antibody-epr16897-145-ab308433'>ab308433</a>) at 1/30 dilution

All lanes:

HCT116 (human colorectal carcinoma epithelial cell) treated with 0.5 mM DFO for 24 hour whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 110 kDa

false

Exposure time: 15s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Clathrin heavy chain was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with ab305259 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305259. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg Lane 2 : ab305259 IP in HeLa whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305259 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 50 seconds

All lanes:

Immunoprecipitation - Anti-Clathrin heavy chain antibody [EPR24236-222] (<a href='/en-us/products/primary-antibodies/clathrin-heavy-chain-antibody-epr24236-222-ab305259'>ab305259</a>) at 1/30 dilution

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 180 kDa

false

Exposure time: 50s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

IFNAR2 was immunoprecipitated from 0.35 mg U266B1 (human multiple myeloma B lymphocyte) whole cell lysate with ab308498 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308498 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : U266B1 (human multiple myeloma B lymphocyte) whole cell lysate Lane 2 : abAB308498 IP in U266B1 (human multiple myeloma B lymphocyte) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab308498 in U266B1 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-IFNAR2 antibody [EPR24706-26] (<a href='/en-us/products/primary-antibodies/ifnar2-antibody-epr24706-26-ab308498'>ab308498</a>) at 1/30 dilution

All lanes:

U266B1 (human multiple myeloma B lymphocyte) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 57 kDa,80 kDa

false

Exposure time: 180s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-MX1 antibody [EPR19967] (<a href='/en-us/products/primary-antibodies/mx1-antibody-epr19967-ab207414'>ab207414</a>) at 1/1000 dilution

Lanes 1 - 2:

Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/mx1-antibody-epr19967-ab207414'>ab207414</a> in Daudi treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Predicted band size: 76 kDa

Observed band size: 76 kDa

false

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

PGAM5 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab308449 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308449 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 2 : abAB308449 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab308449 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-PGAM5 antibody [EPR27145-82] (<a href='/en-us/products/primary-antibodies/pgam5-antibody-epr27145-82-ab308449'>ab308449</a>) at 1/30 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 32 kDa

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SOAT 1/ACAT1 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 µg with ab307597 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307597 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HepG2 whole cell lysate 10 µg Lane 2 : ab307597 IP in HepG2 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307597 in HepG2 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 5 seconds. The molecular weight observed is consistent with what has been described in the literature (PMID : 25617738; 32424158). The bands above 50kDa are dimer and tetramer.

All lanes:

Immunoprecipitation - Anti-SOAT 1/ACAT1 antibody [EPR26952-40] (<a href='/en-us/products/primary-antibodies/soat-1-acat1-antibody-epr26952-40-ab307597'>ab307597</a>) at 1/30 dilution

All lanes:

HepG2 (human hepatocellular carcinoma epithelial cell)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 50 kDa,100 kDa,150 kDa

false

Exposure time: 5s

• IP

AbReview39234****

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

ab6148 Immunoprecipitating IRAK2 in human HEK293 whole cell lysate. 1000μg of cell lysate was incubated with primary antibody (1 μg/mg) and matrix (Protein G) for 16 hours at 4°C. For western blotting a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/10000) was used to confirm successful immunoprecipation.

false

This image is courtesy of an anonymous Abreview.

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

CXCL10/IP-10 was immunoprecipitated from 0.35 mg THP-1 treated with 200ng/ml IFN-y (ab259377) for 24 hours and 50ng/ml LPS for 24 hours, and 5μg/ml Brefeldin A for the last 21 hours whole cell lysate 20 μg with ab306587 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab306587. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : THP-1 treated with 200ng/ml IFN-y (ab259377) for 24 hours and 50ng/ml LPS for 24 hours, and 5μg/ml Brefeldin A for the last 21 hours whole cell lysate 20 μg Lane 2 : ab306587 IP in THP-1 treated with 200ng/ml IFN-y (ab259377) for 24 hours and 50ng/ml LPS for 24 hours, and 5μg/ml Brefeldin A for the last 21 hours Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab306587 in THP-1 treated with 200ng/ml IFN-y (ab259377) for 24 hours and 50ng/ml LPS for 24 hours, and 5μg/ml Brefeldin A for the last 21 hours whole cell lysate 20 μg Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-CXCL10/IP-10 antibody [EPR24674-84] (<a href='/en-us/products/primary-antibodies/cxcl10-ip-10-antibody-epr24674-84-ab306587'>ab306587</a>) at 1/30 dilution

All lanes:

THP-1

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 11 kDa

false

Exposure time: 180s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Histone H2B was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab52484 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab52484 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.  Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate  10 ug Lane 2 : ab52484 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab52484 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 32 seconds.

All lanes:

Immunoprecipitation - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h2b-antibody-mabcam-52484-chip-grade-ab52484'>ab52484</a>) at 1/30 dilution

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Predicted band size: 14 kDa

Observed band size: 15 kDa

false

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Phosphoserine Aminotransferase was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab308512 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308512 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate Lane 2 : abAB308512 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab308512 in HepG2 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 5 seconds

All lanes:

Immunoprecipitation - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (<a href='/en-us/products/primary-antibodies/phosphoserine-aminotransferase-antibody-epr26994-48-ab308512'>ab308512</a>) at 1/30 dilution

All lanes:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 40 kDa

false

Exposure time: 5s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

GATAD2B was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab308631 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308631 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 2 : abAB308631 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab308631 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 50 seconds Lysate was freshly made and used for Immunoprecipitation immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-GATAD2B antibody [EPR27329-3] (<a href='/en-us/products/primary-antibodies/gatad2b-antibody-epr27329-3-ab308631'>ab308631</a>) at 1/30 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 72 kDa

false

Exposure time: 50s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SHP2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug with ab290646 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab290646 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg Lane 2 : ab290646 IP in HeLa whole cell lysate Lane 3 : Mouse IgG1 monoclonal (ab18443) instead of ab290646 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 seconds

Lane 1:

Immunoprecipitation - Anti-SHP2 antibody [79/PTP1D/SHP2] (<a href='/en-us/products/primary-antibodies/shp2-antibody-79-ptp1d-shp2-ab290646'>ab290646</a>) at 1/30 dilution

Lane 2:

Immunoprecipitation - Anti-SHP2 antibody [79/PTP1D/SHP2] (<a href='/en-us/products/primary-antibodies/shp2-antibody-79-ptp1d-shp2-ab290646'>ab290646</a>) at 1/1000 dilution

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Predicted band size: 68 kDa

false

Exposure time: 3s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SPG7/PGN was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 μg with ab305255 at 1/30 dilution (2μg in 0.35mg lysates). VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 μg Lane 2 : ab305255 IP in HEK-293 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305255 in HEK-293 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-SPG7/PGN antibody [EPR26225-9] (<a href='/en-us/products/primary-antibodies/spg7-pgn-antibody-epr26225-9-ab305255'>ab305255</a>) at 1/30 dilution

All lanes:

HEK-293 (human embryonic kidney epithelial cell)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 88 kDa

false

Exposure time: 3min

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

TRBP was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab324950 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324950 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-TRBP antibody [EPR29899-84] (ab324950) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

ab324950 at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 50 kDa

false

Exposure time: 39s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Golgin 97 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab308520 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308520 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate Lane 2 : abAB308520 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab308520 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 24 seconds

All lanes:

Immunoprecipitation - Anti-Golgin 97 antibody [EPR27333-18] (<a href='/en-us/products/primary-antibodies/golgin-97-antibody-epr27333-18-ab308520'>ab308520</a>) at 1/30 dilution

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 97 kDa

false

Exposure time: 24s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

PPAR gamma was immunoprecipitated from 0.35 mg HDLM-2 (human Hodgkin lymphoma) whole cell lysate with ab310323 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab310323 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HDLM-2 (human Hodgkin lymphoma) whole cell lysate Lane 2 : ab310323 IP in HDLM-2 (human Hodgkin lymphoma) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab310323 in HDLM-2 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-PPAR gamma antibody [EPR25862-79] (<a href='/en-us/products/primary-antibodies/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-ab310323'>ab310323</a>) at 1/30 dilution

All lanes:

HDLM-2 (human Hodgkin lymphoma) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

RHOJ was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate with ab317699 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317699 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 2 : ab317699 IP in HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317699 in HUVEC whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-RHOJ antibody [EPR28959-55] (<a href='/en-us/products/primary-antibodies/rhoj-antibody-epr28959-55-ab317699'>ab317699</a>) at 1/30 dilution

All lanes:

HUVEC (human umbilical vein endothelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

PSAP was immunoprecipitated from 0.35 mg PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate with ab324885 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324885 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-PSAP antibody [RM1353] (ab324885) at 1/1000 dilution

Lane 1:

(Input) PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

ab325885 at 1/30 IP in PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab324885 in PC-3 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 60-80 kDa

false

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

CHCHD10 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab308510 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308510 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 2 : abAB308510 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab308510 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 8 seconds

All lanes:

Immunoprecipitation - Anti-CHCHD10 antibody [EPR26966-33] (<a href='/en-us/products/primary-antibodies/chchd10-antibody-epr26966-33-ab308510'>ab308510</a>) at 1/30 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 18 kDa

false

Exposure time: 8s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

CBX1 / HP1 beta was immunoprecipitated from 0.4 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab10811 at 1/50 dilution (2µg in 0.4mg lysates). Western blot was performed on the immunoprecipitate using ab10811 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-CBX1 / HP1 beta antibody [MAC353] (<a href='/en-us/products/primary-antibodies/cbx1-hp1-beta-antibody-mac353-ab10811'>ab10811</a>) at 1/50 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate (Input) at 10 µg

Lane 2:

HepG2 whole cell lysate

Lane 3:

Rat IgG2b isotype control (<a href='/en-us/products/primary-antibodies/rat-igg2b-kappa-monoclonal-rtk4530-isotype-control-ab18541'>ab18541</a>) instead of <a href='/en-us/products/primary-antibodies/cbx1-hp1-beta-antibody-mac353-ab10811'>ab10811</a> in HepG2 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 21 kDa

false

Exposure time: 1s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

beta Catenin non-phospho(active) S45 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with ab305261 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305261 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg Lane 2 : ab305261 IP in HeLa whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305261 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 50 seconds

All lanes:

Immunoprecipitation - Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (<a href='/en-us/products/primary-antibodies/beta-catenin-non-phosphoactive-s45-antibody-epr26155-110-1-ab305261'>ab305261</a>) at 1/30 dilution

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 95 kDa

false

Exposure time: 50s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Ret (phospho Y1015) was immunoprecipitated from 0.35 mg TT (human thyroid carcinoma epithelial cell) treated with 100uM pervanadate for 10 min whole cell lysate with ab324978 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324978 at 1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Ret (phospho Y1015) antibody [EPR26919-93] (<a href='/en-us/products/primary-antibodies/ret-phospho-y1015-antibody-epr26919-93-ab324978'>ab324978</a>) at 1/30 dilution

Lanes 1 - 2:

TT (human thyroid carcinoma epithelial cell) treated with 100uM pervanadate for 10 min whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/ret-phospho-y1015-antibody-epr26919-93-ab324978'>ab324978</a> in TT (human thyroid carcinoma epithelial cell) treated with 100uM pervanadate for 10 min whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 150 kDa

false

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SIRT6 was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab289970 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289970 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2 : ab289970 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab289970 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 7.75 seconds

All lanes:

Immunoprecipitation - Anti-SIRT6 antibody [EPR26255-85] (<a href='/en-us/products/primary-antibodies/sirt6-antibody-epr26255-85-ab289970'>ab289970</a>)

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Predicted band size: 39 kDa

Observed band size: 43 kDa

false

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

PFKP was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab325016 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325016 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-PFKP antibody [EPR28830-157] (<a href='/en-us/products/primary-antibodies/pfkp-antibody-epr28830-157-ab325016'>ab325016</a>) at 1/30 dilution

Lanes 1 - 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/pfkp-antibody-epr28830-157-ab325016'>ab325016</a> in HeLa whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 85 kDa

false

Exposure time: 41s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

IFIT2 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 10 μg with ab305231 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305231 at dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 10 μg Lane 2 : ab305231 IP in A431 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305231 in A431 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-IFIT2 antibody [EPR26404-119] (<a href='/en-us/products/primary-antibodies/ifit2-antibody-epr26404-119-ab305231'>ab305231</a>) at 1/30 dilution

All lanes:

A431 (human epidermoid carcinoma epithelial cell)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 55 kDa

false

Exposure time: 3min

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

UBR7 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 μg with ab305239 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305239 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 μg Lane 2 : ab305239 IP in MCF7 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305239 in MCF7 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 32 seconds The bands beneath the target band (55 kDa) are likely to be degraded target fragments

All lanes:

Immunoprecipitation - Anti-UBR7 antibody [EPR26170-67] (<a href='/en-us/products/primary-antibodies/ubr7-antibody-epr26170-67-ab305239'>ab305239</a>) at 1/30 dilution

All lanes:

MCF7 (human breast adenocarcinoma epithelial cell)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 55 kDa

false

Exposure time: 32s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

RAB8A was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate 10 ug with ab307607 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307607 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : A549 whole cell lysate 10 ug Lane 2 : ab307607 IP in A549 whole cell lysate Lane 3 : Mouse IgG2a monoclonal isotype control (ab18413) instead of ab307607 in A549 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 24 seconds.

All lanes:

Immunoprecipitation - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/rab8a-antibody-mjf-r22-79-3-mouse-igg2a-chimeric-ab307607'>ab307607</a>) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) whole cell lysate 10 μg

Lane 2:

A549 whole cell lysate

Observed band size: 23 kDa

false

Exposure time: 24s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

CISH/CIS was immunoprecipitated from 0.35 mg No-GFP-CD16.NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) whole cell lysate with ab308500 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308500 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : No-GFP-CD16.NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) whole cell lysate Lane 2 : abAB308500 IP in No-GFP-CD16.NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab308500 in No-GFP-CD16.NK-92 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-CISH/CIS antibody [EPR25357-36] (<a href='/en-us/products/primary-antibodies/cish-cis-antibody-epr25357-36-ab308500'>ab308500</a>) at 1/30 dilution

All lanes:

No-GFP-CD16.NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 32 kDa

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

HIF-1 alpha was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) treated with 0.5 mM DFO for 24 hour whole cell lysate with ab308433 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308433 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) treated with 0.5 mM DFO for 24 hour whole cell lysate Lane 2 : abAB308433 IP in HeLa (human cervix adenocarcinoma epithelial cell) treated with 0.5 mM DFO for 24 hour whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab308433 in HeLa treated with 0.5 mM DFO for 24 hour whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-HIF-1 alpha antibody [EPR16897-145] (<a href='/en-us/products/primary-antibodies/hif-1-alpha-antibody-epr16897-145-ab308433'>ab308433</a>) at 1/30 dilution

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell) treated with 0.5 mM DFO for 24 hour whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 110 kDa

false

Exposure time: 10s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

ASAH1 was immunoprecipitated from 0.35 mg SK-MEL-28 (human malignant melanoma cell ) whole cell lysate with ab322109 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322109 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : SK-MEL-28 (human malignant melanoma cell ) whole cell lysate
Lane 2 : ab322109 IP in SK-MEL-28 (human malignant melanoma cell ) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab322109 in SK-MEL-28 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

The IP experiment was performed by ab322109 using SK-MEL-28 lysate. The blot was probed by another anti-ASAH1 antibody (ab282276)(1 : 1000 dilution).

All lanes:

Immunoprecipitation - Anti-ASAH1 antibody [MJF-D29767-517] (<a href='/en-us/products/primary-antibodies/asah1-antibody-mjf-d29767-517-ab322109'>ab322109</a>) at 1/30 dilution

All lanes:

SK-MEL-28 (human malignant melanoma cell ) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

gamma Catenin was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab305241 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305241 at dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-gamma Catenin antibody [15/γ-Catenin] (<a href='/en-us/products/primary-antibodies/gamma-catenin-antibody-15-catenin-ab305241'>ab305241</a>) at 1/1000 dilution

Lane 1:

MCF7 whole cell lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/gamma-catenin-antibody-15-catenin-ab305241'>ab305241</a> at 1/30 IP in MCF7 whole cell lysate at 10 µg

Lane 3:

Mouse IgG2a monoclonal isotype control (<a href='/en-us/products/primary-antibodies/mouse-igg2a-kappa-monoclonal-mopc-173-isotype-control-chip-grade-ab18413'>ab18413</a>) instead of <a href='/en-us/products/primary-antibodies/gamma-catenin-antibody-15-catenin-ab305241'>ab305241</a> in MCF7 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 82 kDa

false

Exposure time: 67s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

BRD2 was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 μg with ab139690 1 : 1000 dilution (0.558 μg/ml). Western blot was performed on the immunoprecipitate using ab139690 at 1/1000 dilution. Capture antibody, 1 : 30 dilution (2μg in 0.35mg lysates).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Lysate was freshly made and used for Immunoprecipitation immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-BRD2 antibody [EPR7642] - ChIP Grade (<a href='/en-us/products/primary-antibodies/brd2-antibody-epr7642-chip-grade-ab139690'>ab139690</a>) at 1/1000 dilution

Lanes 1 - 2:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/brd2-antibody-epr7642-chip-grade-ab139690'>ab139690</a> in HeLa whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 110 kDa

false

Exposure time: 58s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

TGF beta 3 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab308300 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308300 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate Lane 2 : abAB308300 IP in A549 (human lung carcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab308300 in A549 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 24 seconds

All lanes:

Immunoprecipitation - Anti-TGF beta 3 antibody [EPR27093-72] (<a href='/en-us/products/primary-antibodies/tgf-beta-3-antibody-epr27093-72-ab308300'>ab308300</a>) at 1/30 dilution

All lanes:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 4 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 55 kDa

false

Exposure time: 24s