Western Blot Stripping Buffer
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(3 Publications)
- No harsh reagents and does not damage antigens or membranes
- Compatible with nitrocellulose and PVDF membranes
- WB
Supplier Data
Western blot - Western Blot Stripping Buffer (AB282569)
Western Blot Stripping Buffer strips antibodies with minimal antigen loss.
Untreated HeLa cell lysate (4, 2,1 and 0.5 μg) was separated by SDS-PAGE and transfered to either nitrocellulose or PVDF membranes. Blots were probed with an aAnti-GAPDH antibody (Panel A). After stripping with Western Blot Stripping Buffer, no residual signal was detected (Panel B). The blots were then re-probed with an Anti-Stat1 antibody (Panel C).
false
- WB
Supplier Data
Western blot - Western Blot Stripping Buffer (AB282569)
Untreated HeLa cell lysate (4, 2,1 and 0.5 μg) was separated by SDS-PAGE and transfered to either nitrocellulose or PVDF membranes. Blots were probed with an Anti-GAPDH antibody (Panel A). After stripping with Western Blot Stripping Buffer, no residual signal was detected (Panel B). The blots were then re-probed with an Anti-Stat1 antibody (Panel C).
Similar results were obtained with chemilumiescent reagents from 2 other manufacturers.
false
Product details
Western Blot Stripping Buffer (ab282569) uniformly removes antibodies from Western blots so that the blots may be reprobed with different antibodies. Western Blot Stripping Buffer is compatible with nitrocellulose and PVDF membranes.
Stripping and reprobing saves precious samples. The entire protocol takes just 25 minutes and removes bound antibodies without harsh reagents or elevated temperatures; it will not damage antigens or membranes.
Western Blot Stripping Buffer is compatible with most chemiluminescent reagents.
We also offer the Western Blot Marker Pen (ab166858) for blot annotation.
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Product protocols
- Visit the General protocols
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- Download websiteProtocolBooklet|en
Target data
Publications (3)
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International journal of molecular sciences 25: PubMed39456979
2024
Applications
Unspecified application
Species
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Journal of clinical pharmacology 64:1193-1203 PubMed38831713
2024
Applications
Unspecified application
Species
Unspecified reactive species
Stem cell research & therapy 15:66 PubMed38443965
2024
Applications
Unspecified application
Species
Unspecified reactive species
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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