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AB282569

Western Blot Stripping Buffer

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(3 Publications)

Western Blot Stripping Buffer (ab282569) uniformly removes antibodies from Western blots so blots may be reprobed with different antibodies in just 25 minutes.

- No harsh reagents and does not damage antigens or membranes
- Compatible with nitrocellulose and PVDF membranes
2 Images
Western blot - Western Blot Stripping Buffer (AB282569)
  • WB

Supplier Data

Western blot - Western Blot Stripping Buffer (AB282569)

Western Blot Stripping Buffer strips antibodies with minimal antigen loss.

Untreated HeLa cell lysate (4, 2,1 and 0.5 μg) was separated by SDS-PAGE and transfered to either nitrocellulose or PVDF membranes. Blots were probed with an aAnti-GAPDH antibody (Panel A). After stripping with Western Blot Stripping Buffer, no residual signal was detected (Panel B). The blots were then re-probed with an Anti-Stat1 antibody (Panel C).

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Western blot - Western Blot Stripping Buffer (AB282569)
  • WB

Supplier Data

Western blot - Western Blot Stripping Buffer (AB282569)

Untreated HeLa cell lysate (4, 2,1 and 0.5 μg) was separated by SDS-PAGE and transfered to either nitrocellulose or PVDF membranes. Blots were probed with an Anti-GAPDH antibody (Panel A). After stripping with Western Blot Stripping Buffer, no residual signal was detected (Panel B). The blots were then re-probed with an Anti-Stat1 antibody (Panel C).

Similar results were obtained with chemilumiescent reagents from 2 other manufacturers.

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Key facts

Form

Liquid

form

Storage buffer

pH: 2.9 - 3.1 Constituents: 88% Water, 10% Sodium lauryl sulfate

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Product details

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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
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Product protocols

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Target data

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39456979

2024

Mangiferin Represses Inflammation in Macrophages Under a Hyperglycemic Environment Through Nrf2 Signaling.

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Species

Unspecified reactive species

Ravichandran Jayasuriya,Kumar Ganesan,Kunka Mohanram Ramkumar

Journal of clinical pharmacology 64:1193-1203 PubMed38831713

2024

Sodium-Glucose Cotransporter-2 Inhibitor Suppresses Endoplasmic Reticulum Stress and Oxidative Stress in Diabetic Nephropathy Through Nrf2 Signaling: A Clinical and Experimental Study.

Applications

Unspecified application

Species

Unspecified reactive species

Murali Krishna Prasad,Paul S Victor,Goutham V Ganesh,Udayama Juttada,Satyavani Kumpatla,Vijay Viswanathan,Kunka Mohanram Ramkumar

Stem cell research & therapy 15:66 PubMed38443965

2024

Amelioration of diabetic nephropathy in mice by a single intravenous injection of human mesenchymal stromal cells at early and later disease stages is associated with restoration of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing He,Boxin Liu,Xiaofeng Du,Yan Wei,Desheng Kong,Baofeng Feng,Ruiyun Guo,Ernest Amponsah Asiamah,Matthew D Griffin,Sean O Hynes,Sanbing Shen,Yan Liu,Huixian Cui,Jun Ma,Timothy O'Brien
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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