Brefeldin A Solution (1,000X)

Product details

Formulation: Brefeldin A Solution is supplied as a 1000X in DMSO, which should be diluted to 1X in cell culture medium.

Concentration: 5.0 mg/ml

Storage: Store the Brefeldin A Solution (1,000X) between 2°C and 8°C. Note: DMSO freezes at this temperature.

Recommended Usage: Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with brefeldin A for ≤ 24 hours, as this can become toxic for cell viability.

Application: Intracellular staining for flow cytometry

Notes: Completely thaw the solution before use, DMSO freezes at 4°C. We recommend aliquotting the reagent into smaller volumes to avoid repeated freeze-thawing.

Brefeldin A is a protein transport inhibitor that increases intracellular cytokine staining signals by blocking transport processes during activation of a cell. This leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum, and makes Brefeldin A useful in intracellular staining of cytokines.
Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest. Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.