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Cell fractionation kit (standard) allows for the rapid and simple preparation of mitochondrial, cytoplasmic, and nuclear fractions from cultured cells of mammals and other species.


- Cited in over 120 publications
- Quickly isolates mitochondrial, cytoplasmic, and nuclear fractions without the need for mechanical disruption
- Provides highly enriched fractions for monitoring proteins of interest across different cellular compartments
- Useful for studying apoptosis and pro-apoptotic protein movement

Images

Western blot - Cell Fractionation Kit - Standard (AB109719), expandable thumbnail
  • Western blot - Cell Fractionation Kit - Standard (AB109719), expandable thumbnail
  • Western blot - Cell Fractionation Kit - Standard (AB109719), expandable thumbnail

Publications

Key facts

Assay type
Direct

Associated Products

Select an associated product type

1 product for Alternative Product

What's included?

1 Kit
Components
5X SDS Sample Buffer
1 x 10 mL
Buffer A 2x concentrate
1 x 175 mL
Detergent I
1 x 25 µL
Detergent II
1 x 1 mL

Recommended products

Cell fractionation kit (standard) allows for the rapid and simple preparation of mitochondrial, cytoplasmic, and nuclear fractions from cultured cells of mammals and other species.


- Cited in over 120 publications
- Quickly isolates mitochondrial, cytoplasmic, and nuclear fractions without the need for mechanical disruption
- Provides highly enriched fractions for monitoring proteins of interest across different cellular compartments
- Useful for studying apoptosis and pro-apoptotic protein movement

Key facts

Assay type
Direct

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

Abcam's Cell Fractionation Kit (Standard) allows for the rapid and simple preparation of mitochondrial, cytoplasmic and nuclear containing fractions from cultured cells from mammals and other species.

The Cell Fractionation Kit is designed to produce highly enriched fractions that allow for the monitoring of proteins of interest through various cellular compartments. It is not designed to produce purified cellular fractions.

This kit does not require mechanical disruption of the sample. This is important as mechanical disruption can disrupt mitochondrial membranes, causing the biologically-irrelevant release of proteins. This kit is particularly useful in studying apoptosis and the movement of pro-apoptotic proteins such as cytochrome c.

Sufficient materials are provided for fractionation of 1 x108 cells or for preparation of 40 samples, each corresponding to one 100 mm plate at 2.5 x 106 cells/plate.

A companion product is also available which allows for the fractionation of cells grown in 96-well plates: Cell Fractionation Kit HT (Cell Fractionation Kit - High Throughput (HT) ab109718).

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Cell Fractionation Kit - Standard (ab109719), expandable thumbnail

    Western blot - Cell Fractionation Kit - Standard (ab109719)

    Western blot of cytoplasmic, mitchondrial and nuclear fractions.

    Cytosolic (C), mitochondrial (M) and nuclear (N) fractions of HepG2 cells were prepared as described in the Protocol. Fractions were analyzed by Western blotting using Abcam's ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail (Cytochrome c Apoptosis WB Antibody Cocktail ab110415) containing antibodies against mitochondrial matrix (pyruvate dehydrogenase subunit E1α, PDH E1α), mitochondrial inner membrane (F1-ATPase α), mitochondrial intermembrane space (cytochrome c) and cytosolic (glyceraldehyde-3-phosphate dehydrogenase, GAPDH) markers as well as with antibodies against additional mitochondrial matrix (Hsp70) and nuclear (poly (ADP-ribose) polymerase, PARP and SP1) markers, followed by appropriate HRP-conjugated goat secondary antibodies and ECL detection.

  • Western blot - Cell Fractionation Kit - Standard (ab109719), expandable thumbnail

    Western blot - Cell Fractionation Kit - Standard (ab109719)

    Analysis of fractions by Western blot with a plasma membrane antibody cocktail.

    Samples were loaded as follows from left to right: (1) Marker, (2) whole cell lysate, (3) cytosolic fraction lysate, (4) membrane fraction lysate and (5) nuclear fraction lysate. Membrane was blotted with a Plasma Membrane Fractionation WB cocktail Membrane Fraction WB Cocktail ab140365 containing anti-Sodium Potassium ATPase antibody (110 kDa), anti-GAPDH antibody (37 kDa) and anti-Histone H3 (di methyl K9) antibody.

  • Western blot - Cell Fractionation Kit - Standard (ab109719), expandable thumbnail

    Western blot - Cell Fractionation Kit - Standard (ab109719)

    Western blot.

    In this experiment, apoptosis was induced in Jurkat, HeLa and 143B osteosarcoma cells by treatment with staurosporine or in Jurkat cells by FAS. Mitochondrial and cytoplasmic fractions were isolated (using ab109719) and probed using Cytochrome c Apoptosis WB Antibody Cocktail ab110415. As is clear from the gels, cytochrome c has translocated partially in FAS-induced cells and STS-treated osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells. The three control targets allow for verification of the "cleanness" of the cell fractionation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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