AB109719

Cell Fractionation Kit - Standard

Name: Cell fractionation kit - standard (ab109719) | Abcam
Brand: Abcam Limited
SKU: AB109719
Price: 285 USD
Availability: InStock
Rating: 5 (5 reviews)

(5 Reviews)

(136 Publications)

Cell fractionation kit -standard (ab109719) allows for the rapid and simple preparation of mitochondrial, cytoplasmic, and nuclear fractions from cultured cells of mammals and other species.

- Cited in over 120 publications

3 Images

Lab

Western blot - Cell Fractionation Kit - Standard (AB109719)

Western blot of cytoplasmic, mitchondrial and nuclear fractions.

Cytosolic (C), mitochondrial (M) and nuclear (N) fractions of HepG2 cells were prepared as described in the Protocol. Fractions were analyzed by Western blotting using Abcam's ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail (ab110415) containing antibodies against mitochondrial matrix (pyruvate dehydrogenase subunit E1α, PDH E1α), mitochondrial inner membrane (F1-ATPase α), mitochondrial intermembrane space (cytochrome c) and cytosolic (glyceraldehyde-3-phosphate dehydrogenase, GAPDH) markers as well as with antibodies against additional mitochondrial matrix (Hsp70) and nuclear (poly (ADP-ribose) polymerase, PARP and SP1) markers, followed by appropriate HRP-conjugated goat secondary antibodies and ECL detection.

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Western blot - Cell Fractionation Kit - Standard (AB109719)

Analysis of fractions by Western blot with a plasma membrane antibody cocktail.

Samples were loaded as follows from left to right : (1) Marker, (2) whole cell lysate, (3) cytosolic fraction lysate, (4) membrane fraction lysate and (5) nuclear fraction lysate. Membrane was blotted with a Plasma Membrane Fractionation WB cocktail ab140365 containing anti-Sodium Potassium ATPase antibody (110 kDa), anti-GAPDH antibody (37 kDa) and anti-Histone H3 (di methyl K9) antibody.

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Western blot - Cell Fractionation Kit - Standard (AB109719)

Western blot.

In this experiment, apoptosis was induced in Jurkat, HeLa and 143B osteosarcoma cells by treatment with staurosporine or in Jurkat cells by FAS. Mitochondrial and cytoplasmic fractions were isolated (using ab109719) and probed using ab110415. As is clear from the gels, cytochrome c has translocated partially in FAS-induced cells and STS-treated osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells. The three control targets allow for verification of the "cleanness" of the cell fractionation.

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Key facts

Assay type

Direct

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Product details

Abcam's Cell Fractionation Kit (Standard) allows for the rapid and simple preparation of mitochondrial, cytoplasmic and nuclear containing fractions from cultured cells from mammals and other species.

The Cell Fractionation Kit is designed to produce highly enriched fractions that allow for the monitoring of proteins of interest through various cellular compartments. It is not designed to produce purified cellular fractions.

This kit does not require mechanical disruption of the sample. This is important as mechanical disruption can disrupt mitochondrial membranes, causing the biologically-irrelevant release of proteins. This kit is particularly useful in studying apoptosis and the movement of pro-apoptotic proteins such as cytochrome c.

Sufficient materials are provided for fractionation of 1 x10⁸ cells or for preparation of 40 samples, each corresponding to one 100 mm plate at 2.5 x 10⁶ cells/plate.

A companion product is also available which allows for the fractionation of cells grown in 96-well plates: Cell Fractionation Kit HT (ab109718).

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What's included?

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Properties and storage information

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Storage information

Please refer to protocols

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Product protocols

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Target data

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Publications (136)

Recent publications for all applications. Explore the full list and refine your search

The Journal of general virology 106: PubMed40111383

2025

Cytoplasmic retention of dengue virus capsid protein by metformin impairing nuclear transport.

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Ian Carlos Puello-Nakayama,Jonathan Hernandez-Castillo,Juan Manuel Castillo,Daniel Talamás-Lara,Selvin Noé Palacios-Rápalo,Rosa María Del Ángel

Nature communications 16:1094 PubMed39904978

2025

Regulation of senescence-associated secretory phenotypes in osteoarthritis by cytosolic UDP-GlcNAc retention and O-GlcNAcylation.

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Donghyun Kang,Jeeyeon Lee,Geunho Yook,Sehan Jeong,Jungkwon Shin,Mi-Sung Kim,Yi-Jun Kim,Hyeryeon Jung,Jinsung Ahn,Tae Woo Kim,Moon Jong Chang,Chong Bum Chang,Seung-Baik Kang,Won Ho Yang,Yong-Ho Lee,Jin Won Cho,Eugene C Yi,Chanhee Kang,Jin-Hong Kim

Biomedical reports 22:36 PubMed39781042

2025

TLE1 corepressor promotes gefitinib resistance in lung cancer A549 cells via E‑cadherin silencing.

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Xin Yao,Nasir Roberts,Prince Iheukwumere,Alana Carmouche,Renwei Chen,Ma Carmela Dela Cruz,Hector Biliran

Journal of medicinal chemistry 67:19475-19502 PubMed39496093

2024

Optimization of Antiproliferative Properties of Triimine Copper(II) Complexes.

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Katarzyna Choroba,Bartosz Zowiślok,Sławomir Kula,Barbara Machura,Anna M Maroń,Karol Erfurt,Cristiana Marques,Sandra Cordeiro,Pedro V Baptista,Alexandra R Fernandes

Cell death & disease 15:750 PubMed39414773

2024

Venetoclax triggers sublethal apoptotic signaling in venetoclax-resistant acute myeloid leukemia cells and induces vulnerability to PARP inhibition and azacitidine.

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Mahesh Tambe,Sarah Unterberger,Mette C Kriegbaum,Ida Vänttinen,Ezgi June Olgac,Markus Vähä-Koskela,Mika Kontro,Krister Wennerberg,Caroline A Heckman

iScience 27:110622 PubMed39252956

2024

MMP9 drives ferroptosis by regulating GPX4 and iron signaling.

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Flobater I Gawargi,Paras K Mishra

IUBMB life 76:1279-1294 PubMed39166889

2024

Lectins CGL and MTL, representatives of mytilectin family, exhibit different antiproliferative activity in Burkitt's lymphoma cells.

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Alexandra S Kuzmich,Alina P Filshtein,Galina N Likhatskaya,Tatiana Y Gorpenchenko,Irina V Chikalovets,Tatyana O Mizgina,Kuo-Feng Hua,Gunhild von Amsberg,Sergey A Dyshlovoy,Oleg V Chernikov

Journal of cardiovascular translational research 17:1427-1441 PubMed39046654

2024

Inhibition of Hsp90 K284 Acetylation Aalleviates Cardiac Injury After Ischemia-Reperfusion Injury.

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Dongyu Zhan,Na Zhang,Li Zhao,Zhirui Sun,Chunyang Cang

Nature communications 15:5955 PubMed39009594

2024

LARP3, LARP7, and MePCE are involved in the early stage of human telomerase RNA biogenesis.

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Tsai-Ling Kao,Yu-Cheng Huang,Yi-Hsuan Chen,Peter Baumann,Chi-Kang Tseng

Nature 631:857-866 PubMed38987586

2024

Interferon subverts an AHR-JUN axis to promote CXCL13 T cells in lupus.

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Calvin Law,Vanessa Sue Wacleche,Ye Cao,Arundhati Pillai,John Sowerby,Brandon Hancock,Alice Horisberger,Sabrina Bracero,Viktoriya Skidanova,Zhihan Li,Ifeoluwakiisi Adejoorin,Eilish Dillon,Isaac J Benque,Diana Pena Nunez,Daimon P Simmons,Joshua Keegan,Lin Chen,Tina Baker,Phillip Z Brohawn,Hussein Al-Mossawi,Ling-Yang Hao,Brian Jones,Navin Rao,Yujie Qu,Stephen E Alves,A Helena Jonsson,Katharina S Shaw,Ruth Ann Vleugels,Elena Massarotti,Karen H Costenbader,Michael B Brenner,James A Lederer,Judd F Hultquist,Jaehyuk Choi,Deepak A Rao

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Cell Fractionation Kit - Standard

Western blot - Cell Fractionation Kit - Standard (AB109719)

Western blot - Cell Fractionation Kit - Standard (AB109719)

Western blot - Cell Fractionation Kit - Standard (AB109719)

Key facts

Assay type

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (136)

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Primary Antibodies

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Product promise