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Abcam's Cell Fractionation Kit (Standard) allows for the rapid and simple preparation of mitochondrial, cytoplasmic and nuclear containing fractions from cultured cells from mammals and other species.

Assay type

Direct

Images

What's included?

1 Kit
Components
5X SDS Sample Buffer
1 x 10 mL
Buffer A 2x concentrate
1 x 175 mL
Detergent I
1 x 25 µL
Detergent II
1 x 1 mL

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Abcam's Cell Fractionation Kit (Standard) allows for the rapid and simple preparation of mitochondrial, cytoplasmic and nuclear containing fractions from cultured cells from mammals and other species.

Key facts

Assay type

Direct

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Abcam's Cell Fractionation Kit (Standard) allows for the rapid and simple preparation of mitochondrial, cytoplasmic and nuclear containing fractions from cultured cells from mammals and other species.

The Cell Fractionation Kit is designed to produce highly enriched fractions that allow for the monitoring of proteins of interest through various cellular compartments. It is not designed to produce purified cellular fractions.

This kit does not require mechanical disruption of the sample. This is important as mechanical disruption can disrupt mitochondrial membranes, causing the biologically-irrelevant release of proteins. This kit is particularly useful in studying apoptosis and the movement of pro-apoptotic proteins such as cytochrome c.

Sufficient materials are provided for fractionation of 1 x108 cells or for preparation of 40 samples, each corresponding to one 100 mm plate at 2.5 x 106 cells/plate.

A companion product is also available which allows for the fractionation of cells grown in 96-well plates: Cell Fractionation Kit HT (ab109718).

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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