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Cell surface protein isolation kit (ab206998) enables the isolation of cell surface proteins for various downstream applications such as western blotting and other structural and functional studies.

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Assay time

3h

Sample type

Suspension cells, Adherent cells

Images

What's included?

10 Test
Components
DTT II
1 x 100 µL
Lysis Buffer VIII
1 x 6.5 mL
PBS Tablet
1 x 1 Tablet
Quenching Solution I
1 x 10 mL
Streptavidin Beads
1 x 1.5 mL
Sulfo-NHS-SS-Biotin
5 x 1 Vial
TBS Tablet
1 x 1 Tablet
Wash Buffer VII
1 x 12 mL

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Cell surface protein isolation kit (ab206998) enables the isolation of cell surface proteins for various downstream applications such as western blotting and other structural and functional studies.

Key facts

Assay time

3h

Sample type

Suspension cells, Adherent cells

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Cell surface protein isolation kit (ab206998) enables the isolation of cell surface proteins for various downstream applications such as western blotting and other structural and functional studies. The cell surface protein isolation kit provides a simple and efficient method for the isolation of cell surface proteins.

In this method, cells are first labeled with Sulfo-NHS-SS-Biotin, an amine-reactive, thiol-cleavable, biotinylation reagent. Cells are subsequently lysed and the labeled cell surface proteins are isolated using streptavidin beads. The bound proteins are then released from beads by incubating with DTT solution. The biotinylation reagent is cell membrane-impermeable, with an extended spacer arm to reduce steric hindrances associated with streptavidin binding.

Cell surface protein isolation protocol summary:
- remove culture media and wash cells
- add biotin solution and incubate for 30 min
- add quenching solution and incubate for 5 min
- collect cells and spin at 500 g for 3 min
- wash cell pellet twice
- resuspend pellet in lysis buffer and incubate for 30 min
- spin at 10,000 g for 2 min and retain supernatant
- add supernatant to streptavidin beads and incubate for 1 hr
- spin at 800 g for 1 min
- wash beads twice and spin at 800 g
- add elution buffer and incubate for 30 min
- spin down beads and collect supernatant for analysis

This product is manufactured by BioVision, an Abcam company and was previously called K295 Cell Surface Protein Isolation Kit. K295-10 is the same size as the 10 test size of ab206998.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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