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Deproteinizing Sample Preparation Kit - TCA (ab204708) offers a simple method to prepare samples for use in assays to measure small molecules, such as lipids, metabolites, metal ions etc.

Assay time

25m

Sample type

Tissue Lysate, Urine, Tissue Extracts, Whole Blood, Tissue Culture Media

Reactivity data

Application
Purification
Reactivity
Reacts
Dilution info
-
Notes

-

Images

What's included?

200 Test
Components
Neutralization Buffer I
1 x 4 mL
Trichloroacetic Acid Solution
1 x 3 mL

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Deproteinizing Sample Preparation Kit - TCA (ab204708) offers a simple method to prepare samples for use in assays to measure small molecules, such as lipids, metabolites, metal ions etc.

Key facts

Assay time

25m

Sample type

Tissue Lysate, Urine, Tissue Extracts, Whole Blood, Tissue Culture Media

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Storage information

Please refer to protocols

Notes

Deproteinizing Sample Preparation Kit - TCA (ab204708) offers a simple method to prepare samples for use in assays to measure small molecules, such as lipids, metabolites, metal ions etc.

This kit is useful where endogenous enzymes and other proteins would interfere with the assay, or would act directly to affect the level of the analyte to be measured, or would bind the analyte.

In this kit, trichloroacetic acid (TCA) is used to precipitate proteins by drastically lowering the sample pH. After removal of precipitated proteins, the pH of the sample is neutralized with neutralization buffer that is provided in the kit. Small molecules present in the sample can then be easily analyzed without interference from endogenous enzymes and other proteins .

The kit is easy to follow, convenient, and can be utilized for the preparation of a large number of samples in parallel. Samples prepared using this kit can be directly used in various bioassays.

This product is manufactured by BioVision, an Abcam company and was previously called K823 Deproteinizing Sample Preparation Kit II. K823-200 is the same size as the 200 test size of ab204708.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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