Deproteinizing Sample Preparation Kit - TCA ab204708 offers a simple method to prepare samples for use in assays to measure small molecules, such as lipids, metabolites, metal ions etc.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Publications
- Life science alliance 7:2023PARP-1 is a transcriptional rheostat of metabolic and bivalent genes during development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGbolahan Bamgbose,Alexei TulinPubMed 38012002
- Redox biology 68:1029582023A role for the cystathionine-β-synthase /HS axis in astrocyte dysfunction in the aging brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnindya Dey,Pijush Kanti Pramanik,Shailendra Kumar Dhar Dwivedi,Fiifi Neizer-Ashun,Tamas Kiss,Abhrajit Ganguly,Heather Rice,Priyabrata Mukherjee,Chao Xu,Mohiuddin Ahmad,Anna Csiszar,Resham BhattacharyaPubMed 37948927
Key facts
- Assay time
25m
- Sample type
Tissue Lysate, Urine, Tissue Extracts, Whole Blood, Tissue Culture Media
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Purification | Reactivity Reacts | Dilution info - | Notes - |
What's included?
Recommended products
Deproteinizing Sample Preparation Kit - TCA ab204708 offers a simple method to prepare samples for use in assays to measure small molecules, such as lipids, metabolites, metal ions etc.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Assay time
25m
- Sample type
Tissue Lysate, Urine, Tissue Extracts, Whole Blood, Tissue Culture Media
Storage
- Shipped at conditions
Ambient - Cannot Ship with Ice
- Appropriate short-term storage conditions
Ambient
- Appropriate long-term storage conditions
Ambient
- Storage information
Please refer to protocols
Notes
Deproteinizing Sample Preparation Kit - TCA (ab204708) offers a simple method to prepare samples for use in assays to measure small molecules, such as lipids, metabolites, metal ions etc.
This kit is useful where endogenous enzymes and other proteins would interfere with the assay, or would act directly to affect the level of the analyte to be measured, or would bind the analyte.
In this kit, trichloroacetic acid (TCA) is used to precipitate proteins by drastically lowering the sample pH. After removal of precipitated proteins, the pH of the sample is neutralized with neutralization buffer that is provided in the kit. Small molecules present in the sample can then be easily analyzed without interference from endogenous enzymes and other proteins .
The kit is easy to follow, convenient, and can be utilized for the preparation of a large number of samples in parallel. Samples prepared using this kit can be directly used in various bioassays.
This product is manufactured by BioVision, an Abcam company and was previously called K823 Deproteinizing Sample Preparation Kit II. K823-200 is the same size as the 200 test size of ab204708.
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Deproteinizing Sample Preparation Kit - TCA (ab204708)
ab 204708.
Effect of TCA amount on deproteinization:
Aliquots of pooledhuman serum (off the clot, 100 μL) were treated with different amounts of TCA following the kit protocol. Estimated protein concentration before deproteinization: 7.9 g/dL. More than 99% of protein was removed using less than 10 μL of TCA/100 μL human serum. Red solid line = Total protein; blue dotted line = sample treated with TCA; green solid line = sample treated with spin column.
Functional Studies - Deproteinizing Sample Preparation Kit - TCA (ab204708)
ab 204708.
Comparison of analysis of lactate:
Human serum samples (100 μL) were analyzed for lactate concentration using L-Lactate Assay Kit (Colorimetric) (L-Lactate Assay Kit (Colorimetric) ab65331). Untreated samples (purple line) show higher signal when compared to TCA treated samples (red line). However, background signal is very similar in both untreated (green line) and treated (blue line) samples.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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