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AB287876

ExoFACS Kit

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ExoFACS Kit (ab287876) is a user friendly kit designed for exosome isolation from biofluids or cell culture media and FACS analysis of exosome markers. Primary antibody included in the kit is against a common exosomal marker (CD9 or CD63) and can be used as a positive control for protein profiling via FACS analysis. Suitable for human biological fluids and cell culture media.

- Suitable for comprehensive exosome profiling
- No initial exosome purification required
- Lyophilized Exosome Standards for positive control included
- Suitable for multiple marker analyses
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Flow Cytometry - ExoFACS Kit (AB287876)
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Supplier Data

Flow Cytometry - ExoFACS Kit (AB287876)

ExoFACS Kit (ab287876) is a useful tool for exosome protein profiling by using FACS technique. ab287876 was used for a protein marker profile in exosomes derived from different sources. Exosome binding on FACS-beads was performed by incubation at 4°C overnight. Exosome-bead complex is ready to be labeled with fluorophore-conjugated antibodies for specific exosome markers.

Figure shows a profile of expression of three different exosome markers in exosomes purified from Melanoma (MM1), Neuroblastoma (SH) and Glioblastoma (U87) cell supernatants.

Key facts

Detection method

Flow cytometry-fluorescent

Assay Platform

Flow cytometer

Sample types

Cell culture media, Other biological fluids

Assay type

Cell-based

Results type

Quantitative

Reacts with

Human

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Reagents, Controls & Accessories

AB288118

Lyophilized Exosome Standard (Human Plasma)

Flow Cytometry - Lyophilized Exosome Standard (Human Plasma) (AB288118)

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Product details

ExoFACS Kit ab287876 (previously known as ExoFACS kit for Cell Culture Media Exosomes K1236) allows exosome isolation from biofluids or cell culture media and FACS analysis of exosome markers. The kit consists of components for exosome isolation, 4 μm beads for the overall capture of pre-isolated exosomes and lyophilized exosomes from cell culture supernatants or human biological fluids as the positive control. The characterization of exosomal proteins (membrane expressed or internal) is subsequently performed using appropriate detection antibodies against exosome associated antigens.
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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