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Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (ab267478) is a simple immunobead assay for isolation/detection of pre-enriched CD63+ human exosomes from biofluids (plasma, urine) or cell culture media.

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Detection method

Fluorescent

Assay Platform

Flow cytometer

Sample type

Cell culture media

Reactive species

Human

Images

What's included?

25 Test
Components
Anti-CD9 PE (Clone VJ1/20)
1 x 125 µL
Assay Buffer 10X
1 x 10 mL
Incubation Buffer
1 x 1.2 mL
Superparamagnetic capture beads (CD63[TEA3/18])
1 x 125 µL

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Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (ab267478) is a simple immunobead assay for isolation/detection of pre-enriched CD63+ human exosomes from biofluids (plasma, urine) or cell culture media.

Key facts

Detection method

Fluorescent

Assay Platform

Flow cytometer

Sample type

Cell culture media

Reactive species

Human

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (ab267478) is a simple immunobead assay for isolation/detection of pre-enriched CD63+ human exosomes from biofluids (plasma, urine) or cell culture media.

Using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody, the kit provides reproducible results and can be run in parallel to exosome immunophenotyping.

The kit and the measurement of exosomes is useful in the identification of apoptotic cells by flow cytometry.

Visit our FAQs page for tips and troubleshooting.

Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment and the immune response.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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