AB267478

Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9)

Name: Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (ab267478) | Abcam
Brand: Abcam Limited
SKU: AB267478
Price: 625 USD
Availability: InStock

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(2 Publications)

Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (ab267478) is a simple immunobead assay for isolation/detection of pre-enriched CD63⁺ human exosomes from biofluids (plasma, urine) or cell culture media.

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Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (AB267478)

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Supplier Data

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (AB267478)

Cell culture exosomes, pre-enriched using Total Exosome Isolation from PC3 Cell Culture Media (A), were resuspended in PBS and bound to CD63-capture beads during an overnight incubation. The following day the bead-bound exosomes were direct stained with primary detection antibody (CD9-PE) and analyzed by flow cytometry.

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Supplier Data

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (AB267478)

Relationship between background noise and specific signal at different exosome concentrations.

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Supplier Data

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (AB267478)

Dot-plot gating strategy for acquisition and analysis. FSC vs SSC (A) and PerCP vs APC (B)

Key facts

Detection method

Fluorescent

Assay Platform

Flow cytometer

Sample types

Cell culture media

Reacts with

Human

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Product details

Using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody, the kit provides reproducible results and can be run in parallel to exosome immunophenotyping.

The kit and the measurement of exosomes is useful in the identification of apoptotic cells by flow cytometry.

Visit our FAQs page for tips and troubleshooting.

Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment and the immune response.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Saudi dental journal 36:1425-1431 PubMed39619718

2024

The potential of exosomes from adipose-derived stromal-vascular fraction in Increasing Migration Activity of Human Dental Pulp Stromal Cells (in vitro ).

Applications

Unspecified application

Species

Unspecified reactive species

Sylva Dinie Alinda,Anggraini Margono,Indah Yulianto,Ike Dwi Maharti,Reizka Asadelia Rafmawan

BMC immunology 23:37 PubMed35941539

2022

Blockade of exosome generation by GW4869 inhibits the education of M2 macrophages in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yilin Peng,Min Zhao,Yinying Hu,Hongyan Guo,Yanyan Zhang,Yanqin Huang,Lin Zhao,Yong Chai,Zhigang Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9)

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (AB267478)

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (AB267478)

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (AB267478)

Key facts

Detection method

Assay Platform

Sample types

Reacts with

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

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Product promise