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AB267479

Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81)

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(2 Publications)

Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9/CD81) (ab267479) is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD9 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody.
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Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (AB267479)
  • Flow Cyt

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Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (AB267479)

Flow analysis of exosomes.

Cell culture exosomes, pre-enriched using Total Exosome Isolation from PC3 Cell Culture Media (A) and human plasma (B), were resuspended in PBS and bound to CD9-capture beads during an overnight incubation. The following day the bead-bound exosomes were direct stained with primary antibody detection (CD9-PE/CD81-PE) and analyzed by flow cytometry

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (AB267479)
  • Flow Cyt

Supplier Data

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (AB267479)

Dot-plot gating strategy for acquisition and analysis.

FSC vs SSC (A) and FL3 vs FL4 (B).

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (AB267479)
  • Flow Cyt

Supplier Data

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (AB267479)

Dynamic range of the assay analyzed by flow cytometry.

Relationship between background noise and specific signal at different exosome concentrations.

Key facts

Detection method

Fluorescent

Assay Platform

Flow cytometer

Sample types

Plasma, Serum

Reacts with

Human

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Reagents, Controls & Accessories

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Product details

Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9/CD81) (ab267479) is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD9 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.

The kit is intended for the immunoisolation (immunomagnetic or FACS) and Flow Cytometry analysis of pre-enriched CD9+ human exosomes from plasma.

Visit our FAQs page for tips and troubleshooting.

Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment and the immune response.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Exosome", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 11" }, { "reproducibilityType": "Intra", "sample": "Exosome", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10" } ]
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What's included?

{ "values": { "25Test": { "sellingSize": "25 Test", "publicAssetCode":"ab267479-25Test", "assetComponentDetails": [ { "size":"1 x 1.2 mL", "name":"Incubation Buffer", "number":"AB267479-CMP05", "productcode":"" }, { "size":"1 x 125 µL", "name":"CD9+ Capture Beads", "number":"AB267479-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"Assay Buffer 10X", "number":"AB267479-CMP02", "productcode":"" }, { "size":"1 x 125 µL", "name":"Anti-CD81 PE (Clone M38)", "number":"AB267479-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Narra J 4:e1358 PubMed39816111

2025

HUVECs-derived exosomes increase neovascularization and decrease limb necrosis in hindlimb ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Muhamad T Ismail,Dyah W Anggrahini,Sofia M Haryana,Budi Y Setianto

European journal of dentistry 17:173-182 PubMed35728610

2022

Analysis of Thrombin-Activated Platelet-Derived Exosome (T-aPDE) Potential for Dental Pulp Regeneration: In-Vitro Study.

Applications

Unspecified application

Species

Unspecified reactive species

Dini Asrianti Bagio,Indah Julianto,Anggraini Margono,Endang Suprastiwi
View all publications
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Product promise

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