Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9/CD81) (ab267479) is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD9 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody.
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- European journal of dentistry 17:173-1822022Analysis of Thrombin-Activated Platelet-Derived Exosome (T-aPDE) Potential for Dental Pulp Regeneration: In-Vitro Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDini Asrianti Bagio,Indah Julianto,Anggraini Margono,Endang SuprastiwiPubMed 35728610
Key facts
- Detection method
- Fluorescent
- Assay Platform
- Flow cytometer
- Sample type
- Plasma, Serum
- Reactive species
- Human
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Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9/CD81) (ab267479) is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD9 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody.
Key facts
- Detection method
- Fluorescent
- Assay Platform
- Flow cytometer
- Sample type
- Plasma, Serum
- Reactive species
- Human
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9/CD81) (ab267479) is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD9 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.
The kit is intended for the immunoisolation (immunomagnetic or FACS) and Flow Cytometry analysis of pre-enriched CD9+ human exosomes from plasma.
Visit our FAQs page for tips and troubleshooting.
Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment and the immune response.
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3 product images
Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (ab267479)
Dot-plot gating strategy for acquisition and analysis.
FSC vs SSC (A) and FL3 vs FL4 (B).
Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (ab267479)
Flow analysis of exosomes.
Cell culture exosomes, pre-enriched using Total Exosome Isolation from PC3 Cell Culture Media (A) and human plasma (B), were resuspended in PBS and bound to CD9-capture beads during an overnight incubation. The following day the bead-bound exosomes were direct stained with primary antibody detection (CD9-PE/CD81-PE) and analyzed by flow cytometry
Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (ab267479)
Dynamic range of the assay analyzed by flow cytometry.
Relationship between background noise and specific signal at different exosome concentrations.
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