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Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9/CD81) (ab267479) is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD9 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody.

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Images

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (AB267479), expandable thumbnail
  • Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (AB267479), expandable thumbnail
  • Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (AB267479), expandable thumbnail

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Key facts

Detection method
Fluorescent
Assay Platform
Flow cytometer
Sample type
Plasma, Serum
Reactive species
Human

Associated Products

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What's included?

25 Test
Components
Anti-CD81 PE (Clone M38)
1 x 125 µL
Assay Buffer 10X
1 x 10 mL
CD9+ Capture Beads
1 x 125 µL
Incubation Buffer
1 x 1.2 mL

Recommended products

Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9/CD81) (ab267479) is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD9 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody.

Key facts

Detection method
Fluorescent
Assay Platform
Flow cytometer
Sample type
Plasma, Serum
Reactive species
Human

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9/CD81) (ab267479) is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD9 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.

The kit is intended for the immunoisolation (immunomagnetic or FACS) and Flow Cytometry analysis of pre-enriched CD9+ human exosomes from plasma.

Visit our FAQs page for tips and troubleshooting.

Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment and the immune response.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (ab267479), expandable thumbnail

    Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (ab267479)

    Dot-plot gating strategy for acquisition and analysis.

    FSC vs SSC (A) and FL3 vs FL4 (B).

  • Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (ab267479), expandable thumbnail

    Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (ab267479)

    Flow analysis of exosomes.

    Cell culture exosomes, pre-enriched using Total Exosome Isolation from PC3 Cell Culture Media (A) and human plasma (B), were resuspended in PBS and bound to CD9-capture beads during an overnight incubation. The following day the bead-bound exosomes were direct stained with primary antibody detection (CD9-PE/CD81-PE) and analyzed by flow cytometry

  • Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (ab267479), expandable thumbnail

    Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (ab267479)

    Dynamic range of the assay analyzed by flow cytometry.

    Relationship between background noise and specific signal at different exosome concentrations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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