Exosome Isolation and Analysis Kit - Flow Cytometry, Urine (ab267480) is a simple immunobead assay for isolation/detection of pre-enriched CD63+ human exosomes from biofluids (plasma, urine) or cell culture media.
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Key facts
- Detection method
Fluorescent
- Assay Platform
Flow cytometer
- Sample type
Urine
- Reactive species
Human
What's included?
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Exosome Isolation and Analysis Kit - Flow Cytometry, Urine (ab267480) is a simple immunobead assay for isolation/detection of pre-enriched CD63+ human exosomes from biofluids (plasma, urine) or cell culture media.
Key facts
- Detection method
Fluorescent
- Assay Platform
Flow cytometer
- Sample type
Urine
- Reactive species
Human
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Exosome Isolation and Analysis Kit - Flow Cytometry, Urine (ab267480) is a simple immunobead assay for isolation/detection of pre-enriched CD63+ human exosomes from biofluids (plasma, urine) or cell culture media.
Using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody, the kit provides reproducible results and can be run in parallel to exosome immunophenotyping.
The kit and the measurement of exosomes is useful in the identification of apoptotic cells by flow cytometry.
Visit our FAQs page for tips and troubleshooting.
Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment and the immune response.
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3 product images
Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Urine (CD63 / CD9) (ab267480)
Dot-plot gating strategy for acquisition and analysis.
FSC vs SSC (A) and FL3 v s FL4 (B).
Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Urine (CD63 / CD9) (ab267480)
Flow analysis of exosomes.
Cell culture exosomes, pre-enriched using Total Exosome Isolation from PC3 Cell Culture Media (A) and human urine (B), were resuspended in PBS and bound to CD63-capture beads during an overnight incubation. The following day the bead-bound exosomes were direct stained with primary detection antibody (CD9-PE) and analyzed by flow cytometry.
Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Urine (CD63 / CD9) (ab267480)
Dynamic range of the assay analyzed by flow cytometry.
Relationship between background noise and specific signal at different exosome concentrations.
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