Extraction Enhancer Buffer 50X

Abcam's Extraction Enhancer Buffer 50X is formulated for the preparation of cell or tissues extracts for use in immunoassays. We recommend using ab193971 when having difficulty in pipetting lysate or viscous lysate.

The Extraction Enhancer Buffer 50X should be diluted to 1X in the appropriate buffer before use. If required the Extraction Enhancer Buffer 50X can be used in conjunction of Abcam's Extraction Buffer 5X (ab193970).

The Extraction Enhancer Buffer 50X may precipitate when stored at + 4°C. To dissolve, warm briefly at + 37°C and mix gently.

**Instructions for use**If the Extraction Buffer 5X is to be used in conjunction with Extraction Enhancer Buffer 50X (ab193971), then follow the instructions below:*Note: The provided Extraction Buffer 5X contains phosphatase inhibitors and protease inhibitor aprotinin. Additional protease inhibitors can be added if required.* **1X Extraction Buffer + Enhancer**Prepare 1X Extraction Buffer + Enhancer by diluting Extraction Buffer 5X and Extraction Enhancer Buffer 50X to 1X with deionized water. To make 10 mL 1X Extraction Buffer + Enhancer combine 7.8 mL deionized water, 2 mL Extraction Buffer 5X and 200 µL Extraction Enhancer Buffer 50X. Mix thoroughly and gently. **Sample preparation****Preparation of extracts from cell pellets****Preparation of extracts from adherent cells by direct lysis (alternative protocol)** **Preparation of extracts from tissue homogenates**