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AB270519

FASP Protein Digestion Kit

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(19 Publications)

FASP Protein Digestion Kit (ab270519) is used to solubilize whole or fractionated protein samples in SDS-PAGE, digest the protein with trypsin, and analyse the resulting peptides by mass spectrometry.

- Compatible with whole proteome extracts and comprehensive range of biological sample types.
- Maximum loading capacity- 0.4 mg protein per kit.

Key facts

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Product details

FASP Protein Digestion Kit (ab270519) is for researchers who wish to solubilize whole or fractionated protein samples in SDS, digest the protein with trypsin, and analyse the resulting peptides by mass spectrometry.

The FASP Protein Digestion Kit provides the necessary columns and buffers to carry out Universal Sample preparation as developed by Wisniewski, Zoubman, Nagaraj and Mann (2009).

ab270519 is compatible with whole proteome extracts and comprehensive range of biological sample types. The kit is compatible with the common reducing agents.

This product is manufactured by Expedeon, an Abcam company and it was previously called FASP Protein Digestion Kit. Expedeon product code 44250 is the same as the 8-pack size of this product.

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What's included?

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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
Ambient
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Product protocols

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Target data

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Journal of fungi (Basel, Switzerland) 9: PubMed37998859

2023

Traversing the Cell Wall: The Chitinolytic Activity of Extracellular Vesicles Facilitates Their Release.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro F Valdez,Taiane Nascimento de Souza,Jhon Jhamilton Artunduaga Bonilla,Daniel Zamith-Miranda,Alicia Corbellini Piffer,Glauber R S Araujo,Allan J Guimarães,Susana Frases,Alana Kelyene Pereira,Taicia Pacheco Fill,Igor L Estevao,Angel Torres,Igor C Almeida,Joshua D Nosanchuk,Leonardo Nimrichter

PloS one 17:e0278294 PubMed36472974

2022

SARS-CoV-2 spike protein variant binding affinity to an angiotensin-converting enzyme 2 fusion glycoproteins.

Applications

Unspecified application

Species

Unspecified reactive species

Alicia M Matthews,Thomas G Biel,Uriel Ortega-Rodriguez,Vincent M Falkowski,Xin Bush,Talia Faison,Hang Xie,Cyrus Agarabi,V Ashutosh Rao,Tongzhong Ju

mBio 13:e0371821 PubMed35164559

2022

Inhibition of Protein Glycosylation Blocks SARS-CoV-2 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Aitor Casas-Sanchez,Alessandra Romero-Ramirez,Eleanor Hargreaves,Cameron C Ellis,Brian I Grajeda,Igor L Estevao,Edward I Patterson,Grant L Hughes,Igor C Almeida,Tobias Zech,Álvaro Acosta-Serrano

Molecular neurobiology 59:574-589 PubMed34735672

2021

Phosphodiesterase 10A Is a Critical Target for Neuroprotection in a Mouse Model of Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Mustafa C Beker,Ahmet B Caglayan,Serdar Altunay,Elif Ozbay,Nilay Ates,Taha Kelestemur,Berrak Caglayan,Ulkan Kilic,Thorsten R Doeppner,Dirk M Hermann,Ertugrul Kilic

Turkish journal of medical sciences 51:2705-2715 PubMed33356029

2021

The role of circadian rhythm in the regulation of cellular protein profiles in the brain.

Applications

Unspecified application

Species

Unspecified reactive species

Mustafa Çağlar Beker,Ertuğrul Kılıç

iScience 24:103221 PubMed34746695

2021

An integrated multi-omic analysis of iPSC-derived motor neurons from C9ORF72 ALS patients.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Li,Ryan G Lim,Julia A Kaye,Victoria Dardov,Alyssa N Coyne,Jie Wu,Pamela Milani,Andrew Cheng,Terri G Thompson,Loren Ornelas,Aaron Frank,Miriam Adam,Maria G Banuelos,Malcolm Casale,Veerle Cox,Renan Escalante-Chong,J Gavin Daigle,Emilda Gomez,Lindsey Hayes,Ronald Holewenski,Susan Lei,Alex Lenail,Leandro Lima,Berhan Mandefro,Andrea Matlock,Lindsay Panther,Natasha Leanna Patel-Murray,Jacqueline Pham,Divya Ramamoorthy,Karen Sachs,Brandon Shelley,Jennifer Stocksdale,Hannah Trost,Mark Wilhelm,Vidya Venkatraman,Brook T Wassie,Stacia Wyman,Stephanie Yang,Jennifer E Van Eyk,Thomas E Lloyd,Steven Finkbeiner,Ernest Fraenkel,Jeffrey D Rothstein,Dhruv Sareen,Clive N Svendsen,Leslie M Thompson

International journal of molecular sciences 22: PubMed34202585

2021

Immortalization and Characterization of Rat Lingual Keratinocytes in a High-Calcium and Feeder-Free Culture System Using ROCK Inhibitor Y-27632.

Applications

Unspecified application

Species

Unspecified reactive species

Zixing Chen,Wenmeng He,Thomas Chun Ning Leung,Hau Yin Chung

Cell 183:1699-1713.e13 PubMed33188775

2020

Tau PTM Profiles Identify Patient Heterogeneity and Stages of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hendrik Wesseling,Waltraud Mair,Mukesh Kumar,Christoph N Schlaffner,Shaojun Tang,Pieter Beerepoot,Benoit Fatou,Amanda J Guise,Long Cheng,Shuko Takeda,Jan Muntel,Melissa S Rotunno,Simon Dujardin,Peter Davies,Kenneth S Kosik,Bruce L Miller,Sabina Berretta,John C Hedreen,Lea T Grinberg,William W Seeley,Bradley T Hyman,Hanno Steen,Judith A Steen

Scientific reports 9:19082 PubMed31836786

2019

Interaction of melatonin and Bmal1 in the regulation of PI3K/AKT pathway components and cellular survival.

Applications

Unspecified application

Species

Unspecified reactive species

Mustafa C Beker,Berrak Caglayan,Ahmet B Caglayan,Taha Kelestemur,Esra Yalcin,Aysun Caglayan,Ulkan Kilic,Ahmet T Baykal,Russel J Reiter,Ertugrul Kilic

BMC genomics 20:976 PubMed31830917

2019

Genomes and secretomes of Ascomycota fungi reveal diverse functions in plant biomass decomposition and pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jean F Challacombe,Cedar N Hesse,Lisa M Bramer,Lee Ann McCue,Mary Lipton,Samuel Purvine,Carrie Nicora,La Verne Gallegos-Graves,Andrea Porras-Alfaro,Cheryl R Kuske
View all publications
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