Fix & Perm / Cell fixation and permeabilization kit is designed to improve the staining of intracellular markers for flow cytometry by fixing cells and permeabilizing cell membranes, enabling access to cytoplasmic, mitochondrial, nuclear, and other organelle markers.
- Optimizes antibody access to intracellular structures while maintaining cell morphology
- Reduces background while allowing simultaneous addition of permeabilization medium and fluorochrome-labeled antibodies
- Quick and easy: incubate with fixation medium, followed by permeabilization medium and antibody, with minimal washing required
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- Journal of cellular and molecular medicine 27:879-8902023Tumour suppressor PTEN activity is differentially inducible by myo-inositol phosphates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKrzysztof Grzymajlo,Bouchra El Hafny-Rahbi,Claudine KiedaPubMed 36852461
- Clinical oral implants research 34:297-3112023Strontium-modified titanium substrate promotes osteogenic differentiation of MSCs and implant osseointegration via upregulating CDH2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuan Zhou,Ziyu Ge,Lu Song,Jiahui Yan,Xinrui Lang,Yanzhen Zhang,Fuming HePubMed 36691814
- Nature communications 13:13182022Benchmarking of SpCas9 variants enables deeper base editor screens of BRCA1 and BCL2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnabel K Sangree,Audrey L Griffith,Zsofia M Szegletes,Priyanka Roy,Peter C DeWeirdt,Mudra Hegde,Abby V McGee,Ruth E Hanna,John G DoenchPubMed 35288574
- Journal of cachexia, sarcopenia and muscle 13:1277-12882022Self-transducible LRS-UNE-L peptide enhances muscle regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMi-Ock Baek,Hye-Jeong Cho,Do Sik Min,Cheol Soo Choi,Mee-Sup YoonPubMed 35178893
- Aging and disease 12:786-8002021Genome-Wide Screen and Validation of Microglia Pro-Inflammatory Mediators in Stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianhua Wu,Zhuoze Wu,Aodi He,Tongmei Zhang,Ping Zhang,Jing Jin,Sisi Li,Gaigai Li,Xinyan Li,Shiqi Liang,Lei Pei,Rong Liu,Qing Tian,Ximiao He,Youming Lu,Zhouping Tang,Hao LiPubMed 34094642
- Theranostics 11:5585-56042021Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDijie Li,Jin Liu,Chaofei Yang,Ye Tian,Chong Yin,Lifang Hu,Zhihao Chen,Fan Zhao,Ru Zhang,Aiping Lu,Ge Zhang,Airong QianPubMed 33859765
- Vaccines 7:2019Immunological Analysis of a CCHFV mRNA Vaccine Candidate in Mouse Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTouraj Aligholipour Farzani,Katalin Földes,Koray Ergünay,Hakan Gurdal,Aliye Bastug,Aykut OzkulPubMed 31527460
- Experimental and therapeutic medicine 14:753-7582017Human bocavirus induces apoptosis and autophagy in human bronchial epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou-Ping Deng et. al.PubMed 28672995
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
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Fix & Perm / Cell fixation and permeabilization kit is designed to improve the staining of intracellular markers for flow cytometry by fixing cells and permeabilizing cell membranes, enabling access to cytoplasmic, mitochondrial, nuclear, and other organelle markers.
- Optimizes antibody access to intracellular structures while maintaining cell morphology
- Reduces background while allowing simultaneous addition of permeabilization medium and fluorochrome-labeled antibodies
- Quick and easy: incubate with fixation medium, followed by permeabilization medium and antibody, with minimal washing required
Storage
- Shipped at conditions
- Ambient - Cannot Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
- Storage information
- Ambient
Notes
Fix & Perm / Cell Fixation and Permeabilization Kit ab185917 is used to improve the staining of intracellular markers for flow cytometry. It is used to fix cells in suspension and then to permeabilize the cell membranes. It can be used for both cytoplasmic, mitochondrial and nuclear markers, as well as markers in other cellular organelles.
The reagents allow antibodies access to intracellular structures and leave the morphological scatter characteristics of cells intact. The formulation reduces background staining and allows the simultaneous addition of the permeabilization medium and fluorochrome labeled antibodies.
The kit protocol is very simple:
- incubate cells with Fixation Medium for 15 min
- add PBS and spin down cells
- add Permeabilization Medium and antibody to cell pellet
- incubate for 15 min
- wash cells with PBS and analyze
The overwhelming majority of flow cytometry antibodies are suitable for use with this kit (a limited number are sensitive to the fixation step).
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9 product images
Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
Normal blood staining with Fix and Perm.
Double labeling of a normal blood sample treated with ab185917, and immunostained for Lactoferrin (PE) and Lysozyme (FITC).
Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
Normal blood staining with Fix and Perm.
Double labeling of a normal blood sample treated with ab185917, and immunostained for CD3 (PE) and MPO-C2 (FITC).
Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
Normal blood staining with Fix and Perm.
Double labeling of a normal blood sample treated with ab185917, and immunostained for Lactoferrin (PE) and MPO-C2 (FITC).
Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
Normal blood staining with Fix and Perm.
Forward and side scatter pattern of a normal blood sample treated with ab185917.
Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
MPO staining with Fix and Perm.
Immunostaining with anti MPO-FITC conjugate of undifferentiated leukemia cells of AML type.
Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
CD3 staining with Fix and Perm.
Immunostaining with CD3-PE conjugate of surface CD3-negative undifferentiated leukemia cells of T-ALL type.
Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
CD22 staining with Fix and Perm.
Immunostaining with CD22-PE conjugate of undifferentiated leukemia cells of B-ALL type.
Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
Intracellular MPO and Lactoferrin staining with Fix and Perm.
Flow cytometric analysis of normal bone marrow (BM) after fixation and pemeabilization using Fix and Perm kit ab185917, followed by immunostaining for the intra-cellular antigens Lactoferrin and MPO.
Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
Leukemia cell staining with Fix and Perm.
Flow cytrometric analysis of bone marrow (BM) from a leukemia patient after fixation and pemeabilization using ab185917, followed by immunostaining for lactoferrin and MPO-C2.
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