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AB185917

Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry)

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(10 Publications)

Fix & Perm / Cell fixation and permeabilization kit is designed to improve the staining of intracellular markers for flow cytometry by fixing cells and permeabilizing cell membranes, enabling access to cytoplasmic, mitochondrial, nuclear, and other organelle markers.

- Optimizes antibody access to intracellular structures while maintaining cell morphology
- Reduces background while allowing simultaneous addition of permeabilization medium and fluorochrome-labeled antibodies
- Quick and easy: incubate with fixation medium, followed by permeabilization medium and antibody, with minimal washing required

9 Images
Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)

Normal blood staining with Fix and Perm.

Double labeling of a normal blood sample treated with ab185917, and immunostained for Lactoferrin (PE) and Lysozyme (FITC).

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)

Normal blood staining with Fix and Perm.

Double labeling of a normal blood sample treated with ab185917, and immunostained for CD3 (PE) and MPO-C2 (FITC).

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)

CD22 staining with Fix and Perm.

Immunostaining with CD22-PE conjugate of undifferentiated leukemia cells of B-ALL type.

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)

CD3 staining with Fix and Perm.

Immunostaining with CD3-PE conjugate of surface CD3-negative undifferentiated leukemia cells of T-ALL type.

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)

Leukemia cell staining with Fix and Perm.

Flow cytrometric analysis of bone marrow (BM) from a leukemia patient after fixation and pemeabilization using ab185917, followed by immunostaining for lactoferrin and MPO-C2.

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)
  • Flow Cyt

Unknown

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)

Normal blood staining with Fix and Perm.

Forward and side scatter pattern of a normal blood sample treated with ab185917.

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)

Normal blood staining with Fix and Perm.

Double labeling of a normal blood sample treated with ab185917, and immunostained for Lactoferrin (PE) and MPO-C2 (FITC).

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)

Intracellular MPO and Lactoferrin staining with Fix and Perm.

Flow cytometric analysis of normal bone marrow (BM) after fixation and pemeabilization using Fix and Perm kit ab185917, followed by immunostaining for the intra-cellular antigens Lactoferrin and MPO.

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)
  • Flow Cyt

Unknown

Flow Cytometry - Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (AB185917)

MPO staining with Fix and Perm.

Immunostaining with anti MPO-FITC conjugate of undifferentiated leukemia cells of AML type.

Key facts

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Fix & Perm / Cell Fixation and Permeabilization Kit ab185917 is used to improve the staining of intracellular markers for flow cytometry. It is used to fix cells in suspension and then to permeabilize the cell membranes. It can be used for both cytoplasmic, mitochondrial and nuclear markers, as well as markers in other cellular organelles.

The reagents allow antibodies access to intracellular structures and leave the morphological scatter characteristics of cells intact. The formulation reduces background staining and allows the simultaneous addition of the permeabilization medium and fluorochrome labeled antibodies.

The kit protocol is very simple:

- incubate cells with Fixation Medium for 15 min

- add PBS and spin down cells

- add Permeabilization Medium and antibody to cell pellet

- incubate for 15 min

- wash cells with PBS and analyze

The overwhelming majority of flow cytometry antibodies are suitable for use with this kit (a limited number are sensitive to the fixation step).

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What's included?

{ "values": { "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab185917-50Test", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"Fixation Medium (Reagent A)", "number":"AB185917-CMP02" }, { "size":"1 x 5 mL", "name":"Permeabilization Medium (Reagent B)", "number":"AB185917-CMP01" } ] }, "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab185917-200Test", "assetComponentDetails": [ { "size":"4 x 5 mL", "name":"Fixation Medium (Reagent A)", "number":"AB185917-CMP02", "productcode":"" }, { "size":"4 x 5 mL", "name":"Permeabilization Medium (Reagent B)", "number":"AB185917-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
Ambient
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Product protocols

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Target data

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 73:1859-1873 PubMed39235608

2024

Stored RBC transfusions leads to the systemic inflammatory response syndrome in anemic murine neonates.

Applications

Unspecified application

Species

Unspecified reactive species

Balamurugan Ramatchandirin,Marie Amalie Balamurugan,Suneetha Desiraju,Yerin Chung,Boguslaw S Wojczyk,Krishnan MohanKumar

Pain 164:1965-1975 PubMed37027145

2023

Local analgesia of electroacupuncture is mediated by the recruitment of neutrophils and released β-endorphins.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Tao Shi,Wan-Ying Cao,Xiao-Ning Zhang,Hong-Ye Wan,Yang-Shuai Su,Zheng-Yang Qu,Rui Wang,Wei He,Xiang-Hong Jing,Xiao-Yu Wang

Journal of cellular and molecular medicine 27:879-890 PubMed36852461

2023

Tumour suppressor PTEN activity is differentially inducible by myo-inositol phosphates.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Grzymajlo,Bouchra El Hafny-Rahbi,Claudine Kieda

Clinical oral implants research 34:297-311 PubMed36691814

2023

Strontium-modified titanium substrate promotes osteogenic differentiation of MSCs and implant osseointegration via upregulating CDH2.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan Zhou,Ziyu Ge,Lu Song,Jiahui Yan,Xinrui Lang,Yanzhen Zhang,Fuming He

Nature communications 13:1318 PubMed35288574

2022

Benchmarking of SpCas9 variants enables deeper base editor screens of BRCA1 and BCL2.

Applications

Unspecified application

Species

Unspecified reactive species

Annabel K Sangree,Audrey L Griffith,Zsofia M Szegletes,Priyanka Roy,Peter C DeWeirdt,Mudra Hegde,Abby V McGee,Ruth E Hanna,John G Doench

Journal of cachexia, sarcopenia and muscle 13:1277-1288 PubMed35178893

2022

Self-transducible LRS-UNE-L peptide enhances muscle regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Ock Baek,Hye-Jeong Cho,Do Sik Min,Cheol Soo Choi,Mee-Sup Yoon

Aging and disease 12:786-800 PubMed34094642

2021

Genome-Wide Screen and Validation of Microglia Pro-Inflammatory Mediators in Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhua Wu,Zhuoze Wu,Aodi He,Tongmei Zhang,Ping Zhang,Jing Jin,Sisi Li,Gaigai Li,Xinyan Li,Shiqi Liang,Lei Pei,Rong Liu,Qing Tian,Ximiao He,Youming Lu,Zhouping Tang,Hao Li

Theranostics 11:5585-5604 PubMed33859765

2021

Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging.

Applications

Unspecified application

Species

Unspecified reactive species

Dijie Li,Jin Liu,Chaofei Yang,Ye Tian,Chong Yin,Lifang Hu,Zhihao Chen,Fan Zhao,Ru Zhang,Aiping Lu,Ge Zhang,Airong Qian

Vaccines 7: PubMed31527460

2019

Immunological Analysis of a CCHFV mRNA Vaccine Candidate in Mouse Models.

Applications

Unspecified application

Species

Unspecified reactive species

Touraj Aligholipour Farzani,Katalin Földes,Koray Ergünay,Hakan Gurdal,Aliye Bastug,Aykut Ozkul

Experimental and therapeutic medicine 14:753-758 PubMed28672995

2017

Human bocavirus induces apoptosis and autophagy in human bronchial epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

You-Ping Deng,Ying-Juan Liu,Zhan-Qiu Yang,Yan-Jun Wang,Bing-Yan He,Pin Liu
View all publications
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