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AB288085

Fraction-PREP Cell Fractionation Kit

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(2 Publications)

Fraction-PREP Cell Fractionation Kit ab288085 is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Sample types

Suspension cells, Tissue Homogenate, Adherent cells

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Product details

Fraction-PREP Cell Fractionation Kit (ab288085), previously known as FractionPREP™ Cell Fractionation Kit (Cat# K270-50), is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample. The method is fast and simple, needing only 2 hours and no ultracentrifugation involved. All four protein fractions obtained are suitable for many downstream applications such as 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.

This product is manufactured by BioVision, an Abcam company and was previously called K270 FractionPREP™ Cell Fractionation Kit. K270-50 is the same size as the 50 test size of ab288085.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab288085-50Test", "assetComponentDetails": [ { "size":"1 x 1.2 mL", "name":"Lysis Buffer X", "number":"AB288085-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"Extraction Buffer V", "number":"AB288085-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"Extraction Buffer III", "number":"AB288085-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Extraction Buffer VI", "number":"AB288085-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Protease Inhibitor Cocktail I", "number":"AB288085-CMP02", "productcode":"" }, { "size":"1 x 150 µL", "name":"DTT Stock (1M)", "number":"AB288085-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2404961 PubMed39932384

2025

Ribonuclease 1 Induces T-Cell Dysfunction and Impairs CD8 T-Cell Cytotoxicity to Benefit Tumor Growth through Hijacking STAT1.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Hao Yang,Bao-Yue Huang,Hsing-Yu Rao,Peng Ye,Bi Chen,Hao-Ching Wang,Chih-Hung Chung,Heng-Hsiung Wu,Hung-Rong Yen,Shao-Chun Wang,Jong-Ho Cha,Xiuwen Yan,Muh-Hwa Yang,Mien-Chie Hung

Cancers 16: PubMed39061185

2024

Anticancer Effect of PtPHEN, Pt5ME, Pt56ME and Their Platinum(IV)-Dihydroxy Derivatives against Triple-Negative Breast Cancer and Cisplatin-Resistant Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Maria George Elias,Shadma Fatima,Timothy J Mann,Shawan Karan,Meena Mikhael,Paul de Souza,Christopher P Gordon,Kieran F Scott,Janice R Aldrich-Wright
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com