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AB65358

Genomic DNA Isolation Kit

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(14 Publications)

Genomic DNA Isolation Kit (ab65358) is designed as a simple and convenient procedure for rapid isolation of genomic DNA from mammalian cells and tissue samples with high yield and purity within 90 minutes.

- All regents supplied in ready-to-use format
- Extracted genomic DNA is free from protein and RNA
- Suitable to use for PCR, restriction digestion, DNA hybridization, cloning and sequencing

Key facts

Assay time

2h

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Direct

Product details

Abcam's Genomic DNA Isolation Kit provides a simple and convenient procedure for rapid isolation of genomic DNA from mammalian cells and tissue samples with high yield and purity. The novel method requires less than 90 minutes to prepare highly pure genomic DNA. The extracted genomic DNA is free from protein and RNA, and suitable for a variety of applications such as PCR, DNA hybridization, enzyme manipulation, cloning, Southern blot, and array-based experiments.

This product is manufactured by BioVision, an Abcam company and was previously called K281 Genomic DNA Isolation Kit. K281-50 is the same size as the 50 test size of ab65358.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

What's included?

{ "values": { "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab65358-50Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Enzyme B Mix", "number":"AB65358-CMP04", "productcode":"" }, { "size":"2 x 1.8 mL", "name":"Lysis Buffer III", "number":"AB65358-CMP01", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"TE Buffer I", "number":"AB65358-CMP03", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:722 PubMed40346382

2025

Krüppel like factor 7 regulates mitochondrial dynamics balance in myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Cao Wang,Fuxing Zhu,Lan Zhou,Situo Zhang,Ruiqi Wang,Hui Tian,Bosong Zhang,Jiahui Wu,Xiang Xu,Ruixian Jiang,Xiaolu Hou,Jian Liu,Weiming Tian

International journal of molecular sciences 25: PubMed38542166

2024

Beneficial Effects of Fibroblast Growth Factor-1 on Retinal Pigment Epithelial Cells Exposed to High Glucose-Induced Damage: Alleviation of Oxidative Stress, Endoplasmic Reticulum Stress, and Enhancement of Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Hsin-Wei Huang,Chung-May Yang,Chang-Hao Yang

The EMBO journal 43:362-390 PubMed38212381

2024

Control of NAD homeostasis by autophagic flux modulates mitochondrial and cardiac function.

Applications

Unspecified application

Species

Unspecified reactive species

Quanjiang Zhang,Zhonggang Li,Qiuxia Li,Samuel Aj Trammell,Mark S Schmidt,Karla Maria Pires,Jinjin Cai,Yuan Zhang,Helena Kenny,Sihem Boudina,Charles Brenner,E Dale Abel

Toxics 11: PubMed36668787

2023

Can Maternal Exposure to Air Pollution Affect Post-Natal Liver Development?

Applications

Unspecified application

Species

Unspecified reactive species

Yong Song,Ling Chen,Ellen Bennett,Amanda J Wheeler,Katherine Southam,Seiha Yen,Fay Johnston,Graeme R Zosky

Annals of translational medicine 9:1005 PubMed34277805

2021

LncRNA-TCONS_00034812 is upregulated in atherosclerosis and upregulates miR-21 through methylation in vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Lin,Xian Zhang,Chiyuan Zhang,Qiao Jin,Luping Jiang

BMC genomics 21:856 PubMed33267773

2020

Detection of CRISPR-mediated genome modifications through altered methylation patterns of CpG islands.

Applications

Unspecified application

Species

Unspecified reactive species

M Heath Farris,Pamela A Texter,Agustin A Mora,Michael V Wiles,Ellen F Mac Garrigle,Sybil A Klaus,Kristine Rosfjord

Scientific reports 10:20460 PubMed33235237

2020

Addressing the challenges of E-cigarette safety profiling by assessment of pulmonary toxicological response in bronchial and alveolar mucosa models.

Applications

Unspecified application

Species

Unspecified reactive species

Koustav Ganguly,Axel Nordström,Tania A Thimraj,Mizanur Rahman,Malin Ramström,Shanzina I Sompa,Elizabeth Z Lin,Fiona O'Brien,Jeremy Koelmel,Lena Ernstgård,Gunnar Johanson,Krystal J Godri Pollitt,Lena Palmberg,Swapna Upadhyay

Oxidative medicine and cellular longevity 2020:7178123 PubMed33101591

2020

Protective Effect of Polydatin on Jejunal Mucosal Integrity, Redox Status, Inflammatory Response, and Mitochondrial Function in Intrauterine Growth-Retarded Weanling Piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Yanan Chen,Yue Li,Tian Wang

Biomaterials science 8:3147-3163 PubMed32363359

2020

ZBTB20-mediated titanium particle-induced peri-implant osteolysis by promoting macrophage inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Junxiong Qiu,Peng Peng,Min Xin,Zhenkang Wen,Zhong Chen,Sipeng Lin,Manyuan Kuang,Yuan Fu,Guibin Fang,Shixun Li,Changchuan Li,Jiaji Mao,Ling Qin,Yue Ding

Lipids 53:581-588 PubMed30203512

2018

Dietary Unsaturated Fatty Acids Modulate Maternal Dyslipidemia-Induced DNA Methylation and Histone Acetylation in Placenta and Fetal Liver in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Breetha Ramaiyan,Ramaprasad Ravichandra Talahalli
View all publications
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