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UV light is a common source of DNA damage, and can lead to skin cancer and premature aging.

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Images

Key facts

Reactive species

Human

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Reactivity data

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

2 Unit
Components
Control for UV-Treated HeLa Lysate
1 x 200 µg
UV-Treated HeLa Lysate
1 x 200 µg

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UV light is a common source of DNA damage, and can lead to skin cancer and premature aging.

Key facts

Reactive species

Human

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

-80°C

Storage information

-80°C

Notes

UV light is a common source of DNA damage, and can lead to skin cancer and premature aging. Exposure to UV-B and UV-C light leads to the formation of pyrimidine dimers, and to a lesser extent, purine dimers and pyrimidine photophosphates. These dimerized DNA bases are typically removed by the nucleotide excision repair pathway. Failure to repair the damage can induce apoptosis by blocking DNA replication and transcription.

The UV-treated HeLa lysate is designed for use as a western blot positive control when studying UV-induced DNA damage and/or apoptosis. Cells were treated with UV-C light for 1 minute, then cultured for 4 hours before collecting for lysates. Untreated cells were grown under standard cell culture conditions for HeLa cells.

Samples are provided solubilized in an SDS-PAGE loading buffer, supplemented with reducing agent. This sample is ready for SDS-electrophoresis and acts as a positive control in Western blotting applications.

Concentration: HeLa UV-treated lysate, 200 μg at 2.0 mg/mL
HeLa untreated lysate, 200 μg at 2.0 mg/mL

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4 product images

  • Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396), expandable thumbnail

    Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396)

    Figure 1. Western blot with ab136812 Apoptosis Western Blot Cocktail. Lane 1: MW marker

    Lane 2: Untreated HeLa lysate (ab157396)

    Lane 3: UV-treated HeLa lysate (ab157396).


    All lysates at 20 µg per lane.


    Primary antibodies (all lanes): ab136812 Apoptosis Western Blot Cocktail (pro/p17-caspase 3, cleaved-PARP, muscle actin) 1:250 dilution.


    Secondary antibodies (all lanes): Goat polyclonal to Mouse IgG - HRP at 1:5000 dilution and Goat polyclonal to Rabbit IgG - HRP at 1:5000 dilution.


    Developed using the ECL method.

    Predicted band sizes: 89, 42, 32, 17 kDa

    Observed band sizes: 89, 42, 32 kDa

  • Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396), expandable thumbnail

    Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396)

    All lanes: Western blot - Anti-p53 (phospho S15) antibody (AB1431)

    All lanes: Western blot - HeLa whole cell lysate (AB150035)

  • Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396), expandable thumbnail

    Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396)

    Figure 2. Western blot with ab133981 Anti-Caspase 9 antibody. Lane 1: MW marker

    Lane 2: Untreated HeLa lysate (ab157396)

    Lane 3: UV-treated HeLa lysate (ab157396)


    All lysates at 20 µg per lane.


    Primary antibody (all lanes): ab133981 Anti-Caspase 9 antibody at 2 µg/mL.


    Secondary antibodies (all lanes): Goat polyclonal to Mouse IgG - HRP at 1:5000 dilution.


    Developed using the ECL method.

    Predicted band size: 35 kDa

    Observed band size: 25 kDa

  • Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396), expandable thumbnail

    Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396)

    Figure 3. Western blot with ab131385 Apoptosis and DNA Damage Western Blot Cocktail. Lanes 1, 4: MW marker

    Lane 2: Untreated HeLa lysate (ab157396)

    Lane 3: UV-treated HeLa lysate (ab157396).


    All lysates at 20 µg per lane.


    Primary antibodies:


    Lanes 1-3: ab131385 Apoptosis and DNA Damage Western Blot Cocktail (cleaved PARP, GAPDH, H2A.X pSer139) 1:250 dilution

    Lanes 4-6: H2A.X pSer139 antibody.


    Secondary antibodies (all lanes): Goat polyclonal to Mouse IgG - HRP at 1:5000 dilution.


    Developed using the ECL method.

    Predicted band sizes: 15, 36, 89 kDa

    Other bands observed (from H2A.X pSer139 antibody, identities unknown):
    25, 30 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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