UV light is a common source of DNA damage, and can lead to skin cancer and premature aging.
Images
Key facts
- Reactive species
- Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
What's included?
Recommended products
UV light is a common source of DNA damage, and can lead to skin cancer and premature aging.
Key facts
- Reactive species
- Human
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- -80°C
Notes
UV light is a common source of DNA damage, and can lead to skin cancer and premature aging. Exposure to UV-B and UV-C light leads to the formation of pyrimidine dimers, and to a lesser extent, purine dimers and pyrimidine photophosphates. These dimerized DNA bases are typically removed by the nucleotide excision repair pathway. Failure to repair the damage can induce apoptosis by blocking DNA replication and transcription.
The UV-treated HeLa lysate is designed for use as a western blot positive control when studying UV-induced DNA damage and/or apoptosis. Cells were treated with UV-C light for 1 minute, then cultured for 4 hours before collecting for lysates. Untreated cells were grown under standard cell culture conditions for HeLa cells.
Samples are provided solubilized in an SDS-PAGE loading buffer, supplemented with reducing agent. This sample is ready for SDS-electrophoresis and acts as a positive control in Western blotting applications.
Concentration: HeLa UV-treated lysate, 200 μg at 2.0 mg/mL
HeLa untreated lysate, 200 μg at 2.0 mg/mL
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396)
Figure 1. Western blot with Apoptosis Western Blot Cocktail (pro/p17-caspase-3, cleaved PARP1, muscle actin) ab136812 Apoptosis Western Blot Cocktail. Lane 1: MW marker
Lane 2: Untreated HeLa lysate (ab157396)
Lane 3: UV-treated HeLa lysate (ab157396).
All lysates at 20 µg per lane.
Primary antibodies (all lanes): Apoptosis Western Blot Cocktail (pro/p17-caspase-3, cleaved PARP1, muscle actin) ab136812 Apoptosis Western Blot Cocktail (pro/p17-caspase 3, cleaved-PARP, muscle actin) 1:250 dilution.
Secondary antibodies (all lanes): Goat polyclonal to Mouse IgG - HRP at 1:5000 dilution and Goat polyclonal to Rabbit IgG - HRP at 1:5000 dilution.
Developed using the ECL method.
Predicted band sizes: 89, 42, 32, 17 kDa
Observed band sizes: 89, 42, 32 kDa
Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396)
All lanes: Western blot - Anti-p53 (phospho S15) antibody (Anti-p53 (phospho S15) antibody ab1431)
All lanes: Western blot - HeLa whole cell lysate (HeLa whole cell lysate ab150035)
Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396)
Figure 2. Western blot with ab133981 Anti-Caspase 9 antibody. Lane 1: MW marker
Lane 2: Untreated HeLa lysate (ab157396)
Lane 3: UV-treated HeLa lysate (ab157396)
All lysates at 20 µg per lane.
Primary antibody (all lanes): ab133981 Anti-Caspase 9 antibody at 2 µg/mL.
Secondary antibodies (all lanes): Goat polyclonal to Mouse IgG - HRP at 1:5000 dilution.
Developed using the ECL method.
Predicted band size: 35 kDa
Observed band size: 25 kDa
Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396)
Figure 3. Western blot with Apoptosis and DNA Damage (H2A.X(S139) + cleaved PARP1 + Anti-GAPDH) Western Blot Cocktail ab131385 Apoptosis and DNA Damage Western Blot Cocktail. Lanes 1, 4: MW marker
Lane 2: Untreated HeLa lysate (ab157396)
Lane 3: UV-treated HeLa lysate (ab157396).
All lysates at 20 µg per lane.
Primary antibodies:
Lanes 1-3: Apoptosis and DNA Damage (H2A.X(S139) + cleaved PARP1 + Anti-GAPDH) Western Blot Cocktail ab131385 Apoptosis and DNA Damage Western Blot Cocktail (cleaved PARP, GAPDH, H2A.X pSer139) 1:250 dilution
Lanes 4-6: H2A.X pSer139 antibody.
Secondary antibodies (all lanes): Goat polyclonal to Mouse IgG - HRP at 1:5000 dilution.
Developed using the ECL method.
Predicted band sizes: 15, 36, 89 kDa
Other bands observed (from H2A.X pSer139 antibody, identities unknown):
25, 30 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com