HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control

• WB

Supplier Data

Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (AB157396)

All lanes:

Western blot - Anti-p53 (phospho S15) antibody (<a href='/en-us/products/primary-antibodies/p53-phospho-s15-antibody-ab1431'>ab1431</a>)

All lanes:

Western blot - HeLa whole cell lysate (<a href='/en-us/products/cell-lysates/hela-whole-cell-lysate-ab150035'>ab150035</a>)

false

• WB

Supplier Data

Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (AB157396)

Western blot with ab136812 Apoptosis Western Blot Cocktail.

Lane 1 : MW marker
Lane 2 : Untreated HeLa lysate (ab157396)
Lane 3 : UV-treated HeLa lysate (ab157396).

All lysates at 20 µg per lane.

Primary antibodies (all lanes) : ab136812 Apoptosis Western Blot Cocktail (pro/p17-caspase 3, cleaved-PARP, muscle actin) 1 : 250 dilution.

Secondary antibodies (all lanes) : Goat polyclonal to Mouse IgG - HRP at 1 : 5000 dilution and Goat polyclonal to Rabbit IgG - HRP at 1 : 5000 dilution.

Developed using the ECL method.

Predicted band sizes : 89, 42, 32, 17 kDa
Observed band sizes : 89, 42, 32 kDa

true

• WB

Supplier Data

Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (AB157396)

Western blot with ab133981 Anti-Caspase 9 antibody.

Lane 1 : MW marker
Lane 2 : Untreated HeLa lysate (ab157396)
Lane 3 : UV-treated HeLa lysate (ab157396)

All lysates at 20 µg per lane.

Primary antibody (all lanes) : ab133981 Anti-Caspase 9 antibody at 2 µg/mL.

Secondary antibodies (all lanes) : Goat polyclonal to Mouse IgG - HRP at 1 : 5000 dilution.

Developed using the ECL method.

Predicted band size : 35 kDa
Observed band size : 25 kDa

true

• WB

Supplier Data

Western blot - HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (AB157396)

Western blot with ab131385 Apoptosis and DNA Damage Western Blot Cocktail.

Lanes 1, 4 : MW marker
Lane 2 : Untreated HeLa lysate (ab157396)
Lane 3 : UV-treated HeLa lysate (ab157396).

All lysates at 20 µg per lane.

Primary antibodies :
Lanes 1-3 : ab131385 Apoptosis and DNA Damage Western Blot Cocktail (cleaved PARP, GAPDH, H2A.X pSer139) 1 : 250 dilution
Lanes 4-6 : H2A.X pSer139 antibody.

Secondary antibodies (all lanes) : Goat polyclonal to Mouse IgG - HRP at 1 : 5000 dilution.

Developed using the ECL method.

Predicted band sizes : 15, 36, 89 kDa
Other bands observed (from H2A.X pSer139 antibody, identities unknown) : 25, 30 kDa

false