HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control

UV light is a common source of DNA damage, and can lead to skin cancer and premature aging. Exposure to UV-B and UV-C light leads to the formation of pyrimidine dimers, and to a lesser extent, purine dimers and pyrimidine photophosphates. These dimerized DNA bases are typically removed by the nucleotide excision repair pathway. Failure to repair the damage can induce apoptosis by blocking DNA replication and transcription.

The UV-treated HeLa lysate is designed for use as a western blot positive control when studying UV-induced DNA damage and/or apoptosis. Cells were treated with UV-C light for 1 minute, then cultured for 4 hours before collecting for lysates. Untreated cells were grown under standard cell culture conditions for HeLa cells.

Samples are provided solubilized in an SDS-PAGE loading buffer, supplemented with reducing agent. This sample is ready for SDS-electrophoresis and acts as a positive control in Western blotting applications.

Concentration: HeLa UV-treated lysate, 200 μg at 2.0 mg/mL
HeLa untreated lysate, 200 μg at 2.0 mg/mL