HeLa whole cell extracts were collected in lysis buffer after 3 hour treatment with Calyculin A (50 nM) or vehicle (DMSO) in serum-free media.
Images
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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HeLa whole cell extracts were collected in lysis buffer after 3 hour treatment with Calyculin A (50 nM) or vehicle (DMSO) in serum-free media.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- -80°C
Notes
HeLa whole cell extracts were collected in lysis buffer after 3 hour treatment with Calyculin A (50 nM) or vehicle (DMSO) in serum-free media. The protein content was determined by BCA assay. Calyculin A is a potent phosphatase inhibitor. Unlike Okadaic Acid, which only acts on protein phosphatase PP2A, Calyculin A inhibits both PP1 and PP2A. Cells treated with Calyculin A accumulate serine/threonine phosphorylation making them a strong positive control for phospho-threonine or phospho-serine specific antibodies.
Concentration:
HeLa Lysate - Vehicle Treated Control - 200 μg at 1 mg/mL
HeLa Lysate - Calyculin Treated - 200 μg at 1 mg/mL
The lysate is supplied in SDS-PAGE loading buffer with DTT and is ready to load on a gel.
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2 product images
Western blot - HeLa Ser/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated (ab135395)
Western Blot.
All lanes: Anti-Phosphoserine/Threonine antibody (Anti-Phospho - (Ser/Thr) Phe antibody ab17464) at 1/1000
Lane 1: Marker
Lane 2: HeLa Whole Cell Lysate - Calyculin Treated at 20 μg
Lane 3: HeLa Whole Cell Lysate – Untreated Control at 20 μg
Secondary:
Goat polyclonal to Rabbit IgG – Pre-Adsorbed (HRP) at 1/6000 developed using the ECL technique.
Performed under reducing conditions.
SDS-PAGE - HeLa Ser/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated (ab135395)
SDS-PAGE. Lane 1: Marker
Lane 2: HeLa Whole Cell Lysate - Calyculin Treated at 20 µg
Lane 3: HeLa Whole Cell Lysate – Untreated control at 20 µg
Gel
10-20% acrylamide separation gel
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