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AB135395

HeLa Ser/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated

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HeLa whole cell extracts were collected in lysis buffer after 3 hour treatment with Calyculin A (50 nM) or vehicle (DMSO) in serum-free media.
2 Images
Western blot - HeLa Ser/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated (AB135395)
  • WB

Supplier Data

Western blot - HeLa Ser/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated (AB135395)

Western Blot.

All lanes : Anti-Phosphoserine/Threonine antibody (ab17464) at 1/1000

Lane 1 : Marker
Lane 2 : HeLa Whole Cell Lysate - Calyculin Treated at 20 μg
Lane 3 : HeLa Whole Cell Lysate – Untreated Control at 20 μg


Secondary :
Goat polyclonal to Rabbit IgG – Pre-Adsorbed (HRP) at 1/6000 developed using the ECL technique.

Performed under reducing conditions.

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SDS-PAGE - HeLa Ser/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated (AB135395)
  • SDS-PAGE

Supplier Data

SDS-PAGE - HeLa Ser/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated (AB135395)

SDS-PAGE. Lane 1 : Marker
Lane 2 : HeLa Whole Cell Lysate - Calyculin Treated at 20 µg
Lane 3 : HeLa Whole Cell Lysate – Untreated control at 20 µg


Gel
10-20% acrylamide separation gel

Key facts

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

HeLa whole cell extracts were collected in lysis buffer after 3 hour treatment with Calyculin A (50 nM) or vehicle (DMSO) in serum-free media. The protein content was determined by BCA assay. Calyculin A is a potent phosphatase inhibitor. Unlike Okadaic Acid, which only acts on protein phosphatase PP2A, Calyculin A inhibits both PP1 and PP2A. Cells treated with Calyculin A accumulate serine/threonine phosphorylation making them a strong positive control for phospho-threonine or phospho-serine specific antibodies.

Concentration:

HeLa Lysate - Vehicle Treated Control - 200 μg at 1 mg/mL
HeLa Lysate - Calyculin Treated - 200 μg at 1 mg/mL

The lysate is supplied in SDS-PAGE loading buffer with DTT and is ready to load on a gel.

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What's included?

{ "values": { "2Unit": { "sellingSize": "2 Unit", "publicAssetCode":"ab135395-2Unit", "assetComponentDetails": [ { "size":"1 x 200 µg", "name":"Control for Calyculin-Treated HeLa Lysate", "number":"AB135395-CMP02", "productcode":"" }, { "size":"1 x 200 µg", "name":"Calyculin-Treated HeLa Lysate", "number":"AB135395-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
-80°C
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Product protocols

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Target data

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