HeLa cells are an adherent human epithelial cell line derived from a cervical carcinoma.
Images
Key facts
- Sample type
- Cell Lysate
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application BL | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application IP | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
Recommended products
HeLa cells are an adherent human epithelial cell line derived from a cervical carcinoma.
Key facts
- Sample type
- Cell Lysate
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- -80°C
Notes
HeLa cells are an adherent human epithelial cell line derived from a cervical carcinoma. HeLa cells were lysed using radio immunoprecipitation assay (RIPA) buffer supplemented with protease and phosphatase inhibitors, centrifuged and the supernatant collected. Protein concentration of the extract was determined by BCA assay. This products is suitable for sandwich ELISAs, Immunoprecipitation, SDS-PAGE and Western Blotting.
Avoid freeze-thaw cycles with this product.
Concentration: Each unit contains a minimum of 300 μg at a lot-specific concentration. See label for concentration.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
ELISA - HeLa Whole Cell RIPA Extract (ab170197)
Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574) using HeLa Whole Cell RIPA Extract.
Glucose 6 Phosphate Isomerase (G6PI) protein was assayed in HeLa Whole Cell RIPA Extract (ab170197) in triplicate using Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574). A standard curve generated using the G6PI standard protein is shown (left image). G6PI signal using 2-fold serial dilutions of ab170197 starting at 50 μg/mL (right image). The G6PI concentration in the HeLa Whole Cell RIPA Extract was interpolated to be 24.9 μg/mL.
ELISA - HeLa Whole Cell RIPA Extract (ab170197)
mtTFA (TFAM) Human ELISA Kit (ab123455) using HeLa Whole Cell RIPA Extract.
Mitochondrial Transcription Factor A (mtTFA) protein was assayed in HeLa Whole Cell RIPA Extract (ab170197) in triplicate using mtTFA (TFAM) Human ELISA Kit (ab123455). A standard curve generated using the mtTFA standard protein is shown (left panel). mtTFA signal using 2-fold serial dilutions of ab170197 starting at 400 μg/mL is shown (right panel). The mtTFA concentration in the HeLa Whole Cell RIPA Extract was interpolated to be 841 ng/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com