His-Tag Protein Expression Check Kit

His-Tag Protein Expression Check Kit (ab270048) is a quick competitive immunochromatography test that allows users to verify and monitor successful expression of His-tagged recombinant proteins before undertaking purification, as well as identifying fractions of most interest before performing other more labor intensive techniques such as Western blot or ELISA. The assay provides a qualitative yes/no answer, although a semi-quantitative measurement of the protein abundance in the sample can be determined using internal references and controls.

A His-tagged protein on the "Test line" (T line) and a ULFA-tagged protein on the "Control line" (C line) are immobilized on the nitrocellulose strips.

40nm Gold conjugated anti-HisTag antibody and 40 nm Gold conjugated anti-ULFA tag conjugates are dried on the conjugate pad and will selectively bind to the T and C line, respectively. The C line is assay-independent and should always appear as a strong red line; if it is not visible, the test is not valid and should be repeated.

His-Tag Protein Expression Check Kit is formulated as a competitive assay so that when the sample does not contain any His-tagged protein, the T line will appear as a strong red line. On the contrary, the T line will not be visible (or appear as a pale red line) if the sample contains the His- tagged protein that competes with the binding of the Gold conjugated anti-HisTag antibody conjugate to the antigen immobilized on the T line.

Benefits:

Quick and easy qualitative detection of His-tagged proteins

Compatible with cell culture media and lysate

No costly equipment required

Small sample volumes required (80 μL)

Compatible with Ni-NTA Affinity Resin - Amintra® (Ni-NTA Affinity Resin - Amintra ab270549)

This product is manufactured by Expedeon, an Abcam company. It was previously called His-Tag Check&Go!. Expedeon product code 4003-0030 is the same as the 30-test size of this product.