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His-Tag Protein Expression Check Kit (ab270048) is a quick competitive immunochromatography test that allows users to verify and monitor successful expression of His-tagged recombinant proteins before undertaking purification, as well as identifying fractions of most interest before performing other more labor intensive techniques such as Western blot or ELISA.

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What's included?

30 Test
Components
His-Tag Protein Expression Check Strips
1 x 30 Test
Running Buffer (10X)
1 x 1500 µL

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His-Tag Protein Expression Check Kit (ab270048) is a quick competitive immunochromatography test that allows users to verify and monitor successful expression of His-tagged recombinant proteins before undertaking purification, as well as identifying fractions of most interest before performing other more labor intensive techniques such as Western blot or ELISA.

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

His-Tag Protein Expression Check Kit (ab270048) is a quick competitive immunochromatography test that allows users to verify and monitor successful expression of His-tagged recombinant proteins before undertaking purification, as well as identifying fractions of most interest before performing other more labor intensive techniques such as Western blot or ELISA. The assay provides a qualitative yes/no answer, although a semi-quantitative measurement of the protein abundance in the sample can be determined using internal references and controls.

A His-tagged protein on the "Test line" (T line) and a ULFA-tagged protein on the "Control line" (C line) are immobilized on the nitrocellulose strips.

40nm Gold conjugated anti-HisTag antibody and 40 nm Gold conjugated anti-ULFA tag conjugates are dried on the conjugate pad and will selectively bind to the T and C line, respectively. The C line is assay-independent and should always appear as a strong red line; if it is not visible, the test is not valid and should be repeated.

His-Tag Protein Expression Check Kit is formulated as a competitive assay so that when the sample does not contain any His-tagged protein, the T line will appear as a strong red line. On the contrary, the T line will not be visible (or appear as a pale red line) if the sample contains the His- tagged protein that competes with the binding of the Gold conjugated anti-HisTag antibody conjugate to the antigen immobilized on the T line.

Benefits:

Quick and easy qualitative detection of His-tagged proteins

Compatible with cell culture media and lysate

No costly equipment required

Small sample volumes required (80 μL)

Compatible with Ni-NTA Affinity Resin - Amintra® (ab270549)

This product is manufactured by Expedeon, an Abcam company. It was previously called His-Tag Check&Go!. Expedeon product code 4003-0030 is the same as the 30-test size of this product.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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