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Histone Extraction Kit (ab113476) is suitable for a quick isolation of total histone extracts from cells and tissue samples from mammals and other species.

Assay time

1h

Sample type

Tissue, Suspension cells, Adherent cells

Images

What's included?

100 Test
Components
10X Pre-Lysis Buffer
1 x 10 mL
Balance Buffer
1 x 8 mL
DTT Solution
1 x 20 µL
Lysis Buffer
1 x 20 mL

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Histone Extraction Kit (ab113476) is suitable for a quick isolation of total histone extracts from cells and tissue samples from mammals and other species.

Key facts

Assay time

1h

Sample type

Tissue, Suspension cells, Adherent cells

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Histone Extraction Kit (ab113476) is suitable for a quick isolation of total histone extracts from cells and tissue samples from mammals and other species.

The amount of starting material can be as low as 105 cells or 1 mg of tissue. For best results, the cell number should be >106 cells or the tissue amount should be >10 mg.

A total of 100 standard extractions (using 107 cells or 100 mg of tissue per extraction) can be performed with this kit. Yield of the total histone proteins can be up to 0.4 mg per 107 cells or per 100 mg of tissue. The yield may vary depending on the cell or tissue type.

Histone purification is most often performed before the analysis of the global levels within a sample of post-translational histone modifications in western blot.

Chromatin immunoprecipitation (ChIP) is the most common technique used for the analysis of histone modifications at specific sites within the genome. Use a chromatin extraction kit instead of a histone purification kit for the preparation of samples for ChIP.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

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