Histone Extraction Kit

Product details

Histone Extraction Kit (ab113476) is suitable for a quick isolation of total histone extracts from cells and tissue samples from mammals and other species.

The amount of starting material can be as low as 10⁵ cells or 1 mg of tissue. For best results, the cell number should be >10⁶ cells or the tissue amount should be >10 mg.

A total of 100 standard extractions (using 10⁷ cells or 100 mg of tissue per extraction) can be performed with this kit. Yield of the total histone proteins can be up to 0.4 mg per 10⁷ cells or per 100 mg of tissue. The yield may vary depending on the cell or tissue type.

Histone purification is most often performed before the analysis of the global levels within a sample of post-translational histone modifications in western blot.

Chromatin immunoprecipitation (ChIP) is the most common technique used for the analysis of histone modifications at specific sites within the genome. Use a chromatin extraction kit instead of a histone purification kit for the preparation of samples for ChIP.

REACH authorisation
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