Histone extraction kit is designed for the quick isolation of total histone extracts from cells and tissue samples within 1 hour assay time, suitable for mammalian and other species.
- Cited in over 110 publications
- Isolates histones from as few as 10^5 cells or 1 mg of tissue, with optimal results from >10^6 cells or >10 mg of tissue
- High yield – up to 0.4 mg of histone protein per 10^7 cells or 100 mg of tissue
- Useful in western blot analysis of histone modifications and post-translational modifications
- Not for ChIP; use a chromatin extraction kit for ChIP preparation
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- International journal of obesity (2005) 48:557-5662023SMYD3: a new regulator of adipocyte precursor proliferation at the early steps of differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatjana Sajic,Chayenne Karine Ferreira Gomes,Marie Gasser,Tiziana Caputo,Nasim Bararpour,Esther Landaluce-Iturriria,Marc Augsburger,Nadia Walter,Alexandre Hainard,Isabel C Lopez-Mejia,Tony Fracasso,Aurélien Thomas,Federica GilardiPubMed 38148333
- Nature cell biology 25:1821-18322023Prostate lineage-specific metabolism governs luminal differentiation and response to antiandrogen treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJenna M Giafaglione,Preston D Crowell,Amelie M L Delcourt,Takao Hashimoto,Sung Min Ha,Aishwarya Atmakuri,Nicholas M Nunley,Rachel M A Dang,Mao Tian,Johnny A Diaz,Elisavet Tika,Marie C Payne,Deborah L Burkhart,Dapei Li,Nora M Navone,Eva Corey,Peter S Nelson,Neil Y C Lin,Cedric Blanpain,Leigh Ellis,Paul C Boutros,Andrew S GoldsteinPubMed 38049604
- Life science alliance 7:2023Transgenerational epigenetic effects imposed by neonicotinoid thiacloprid exposure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOuzna Dali,Shereen D'Cruz,Louis Legoff,Mariam Diba Lahmidi,Celine Heitz,Pierre-Etienne Merret,Pierre-Yves Kernanec,Farzad Pakdel,Fatima SmagulovaPubMed 37973188
- Cells 12:2023The MYST Family Histone Acetyltransferase SasC Governs Diverse Biological Processes in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesJae-Yoon Kwon,Young-Ho Choi,Min-Woo Lee,Jae-Hyuk Yu,Kwang-Soo ShinPubMed 37998377
- Cell death & disease 14:6862023MacroH2A1.1 as a crossroad between epigenetics, inflammation and metabolism of mesenchymal stromal cells in myelodysplastic syndromes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC Giallongo,I Dulcamare,S Giallongo,A Duminuco,D Pieragostino,M C Cufaro,A M Amorini,G Lazzarino,A Romano,N Parrinello,M Di Rosa,G Broggi,R Caltabiano,M Caraglia,M Scrima,L S Pasquale,M S Tathode,G Li Volti,R Motterlini,F Di Raimondo,D Tibullo,G A PalumboPubMed 37852977
- Frontiers in oncology 13:12293122023VRK3 depletion induces cell cycle arrest and metabolic reprogramming of pontine diffuse midline glioma - H3K27 altered cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVirginie Menez,Thomas Kergrohen,Tal Shasha,Claudia Silva-Evangelista,Ludivine Le Dret,Lucie Auffret,Chloé Subecz,Manon Lancien,Yassine Ajlil,Irma Segoviano Vilchis,Kévin Beccaria,Thomas Blauwblomme,Estelle Oberlin,Jacques Grill,David Castel,Marie-Anne DebilyPubMed 37886173
- Cells 12:2023Normalization of the ATP1A1 Signalosome Rescinds Epigenetic Modifications and Induces Cell Autophagy in Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPradeep Kumar Rajan,Utibe-Abasi S Udoh,Yuto Nakafuku,Sandrine V Pierre,Juan SanabriaPubMed 37830582
- Nature metabolism 5:1747-17642023Glutarate regulates T cell metabolism and anti-tumour immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEleanor Minogue,Pedro P Cunha,Brennan J Wadsworth,Guinevere L Grice,Shiv K Sah-Teli,Rob Hughes,David Bargiela,Alessandro Quaranta,Javier Zurita,Robin Antrobus,Pedro Velica,Laura Barbieri,Craig E Wheelock,Peppi Koivunen,James A Nathan,Iosifina P Foskolou,Randall S JohnsonPubMed 37605057
- Journal of translational medicine 21:5472023Warburg effect enhanced by AKR1B10 promotes acquired resistance to pemetrexed in lung cancer-derived brain metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenzhe Duan,Wenwen Liu,Shengkai Xia,Yang Zhou,Mengyi Tang,Mingxin Xu,Manqing Lin,Xinyu Li,Qi WangPubMed 37587486
- Molecular carcinogenesis 62:1717-17302023Targeting BRPF3 moderately reverses olaparib resistance in high grade serous ovarian carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin G Bitler,Courtney A Bailey,Tomomi M Yamamoto,Alexandra McMellen,Hyunmin Kim,Zachary L WatsonPubMed 37493106
Key facts
- Assay time
- 1h
- Sample type
- Tissue, Suspension cells, Adherent cells
What's included?
Recommended products
Histone extraction kit is designed for the quick isolation of total histone extracts from cells and tissue samples within 1 hour assay time, suitable for mammalian and other species.
- Cited in over 110 publications
- Isolates histones from as few as 10^5 cells or 1 mg of tissue, with optimal results from >10^6 cells or >10 mg of tissue
- High yield – up to 0.4 mg of histone protein per 10^7 cells or 100 mg of tissue
- Useful in western blot analysis of histone modifications and post-translational modifications
- Not for ChIP; use a chromatin extraction kit for ChIP preparation
Key facts
- Assay time
- 1h
- Sample type
- Tissue, Suspension cells, Adherent cells
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Please refer to protocols
Notes
Histone Extraction Kit (ab113476) is suitable for a quick isolation of total histone extracts from cells and tissue samples from mammals and other species.
The amount of starting material can be as low as 105 cells or 1 mg of tissue. For best results, the cell number should be >106 cells or the tissue amount should be >10 mg.
A total of 100 standard extractions (using 107 cells or 100 mg of tissue per extraction) can be performed with this kit. Yield of the total histone proteins can be up to 0.4 mg per 107 cells or per 100 mg of tissue. The yield may vary depending on the cell or tissue type.
Histone purification is most often performed before the analysis of the global levels within a sample of post-translational histone modifications in western blot.
Chromatin immunoprecipitation (ChIP) is the most common technique used for the analysis of histone modifications at specific sites within the genome. Use a chromatin extraction kit instead of a histone purification kit for the preparation of samples for ChIP.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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4 product images
Functional Studies - Histone Extraction Kit (ab113476)
Histone extracts were prepared from MCF-7 cells using the ab113476 and acetyl histone H3-K9 was quantified.
Nowacka-Woszuk, Joanna et al., PloS one: vol. 13,2 e0193464., Fig 4, doi:10.1371/journal.pone.0193464Functional Studies - Histone Extraction Kit (ab113476)
The differences in H3 acetylation level in fetal livers (female fetuses aged 19 dpc) from F1, F2, and F3 generations. measured with ab113476. Histones were prepared using Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) ab115124
Asterisk indicate a significant difference between the respective C and R groups: ∗∗ P < 0.01; NS not significant. Data presented as the means ± SE (n = 6 per group).
Functional Studies - Histone Extraction Kit (ab113476)
SDS-PAGE Analysis of samples prepared using Histone extraction Kit.
Functional Studies - Histone Extraction Kit (ab113476)
Functional Studies - Histone Extraction Kit.
Histones were purified from mouse liver cells using ab113476. Each lane was loaded with 15μg of purified histone.
Image courtesy of Kevin Dalgaard, MPI for Immunobiology & Epigenetics, Freiburg, DE
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