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The Human B Cell Isolation Kit was developed with BACS™ Microbubbles for the enrichment of untouched human B cells. Unwanted cells are depleted through negative selection.

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Images

Flow Cytometry - Human B Cell Isolation Kit (AB322031), expandable thumbnail

Key facts

Assay time

30m

Assay type

Cell-based (quantitative)

Reactive species

Human

Reactivity data

Application

Flow Cyt

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

1000000000 Cells/vial
Components
5 mL Tubes
20 x 1 Vial
BACS Streptavidin Microbubbles
1 x 10.5 mL
Human B Cell Biotin Antibody Cocktail
1 x 1050 µL
Separation Buffer
1 x 200 mL

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The Human B Cell Isolation Kit was developed with BACS™ Microbubbles for the enrichment of untouched human B cells. Unwanted cells are depleted through negative selection.

Key facts

Assay time

30m

Assay type

Cell-based (quantitative)

Reactive species

Human

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

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The Human B Cell Isolation Kit was developed with BACS™ Microbubbles for the enrichment of untouched human B cells. Unwanted cells are depleted through negative selection.

  • This kit can process up to 1 billion MNCs.
  • Fast & easy workflow enriches human mononuclear cells (MNCs) for B cells in 30 minutes
  • No magnet, no column, no harsh chemicals
  • Microbubble enrichment maintains cell health and physiology

Mechanism of Action: The Human B Cell Isolation Kit uses a negative selection method to remove unwanted cell populations and effectively enrich B cells. Non-B cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD2, CD3, CD14, CD15, CD16, CD36, CD43, CD56, CD57, CD123, CD161, CD235ab, and CD314.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - Human B Cell Isolation Kit (ab322031), expandable thumbnail

    Flow Cytometry - Human B Cell Isolation Kit (ab322031)

    Untouched human B cells were isolated from PBMCs using the Human B Cell Isolation Kit. Isolated cells were labeled with CD3-FITC and CD19-APC/Fire750. The fluorescently labeled cells were analyzed by flow cytometry. Dead cells/debris were excluded from analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com