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Human CD4+ T Cell Isolation Kit delivers a highly enriched population of healthy, viable CD4+ T cells using a protocol that is fast, easy, and exceptionally gentle on delicate immune cells. 

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Images

Flow Cytometry - Human CD4+ T Cell Isolation Kit (AB322033), expandable thumbnail

Key facts

Assay time

30m

Assay type

Cell-based (quantitative)

Reactive species

Human

Reactivity data

Application

Flow Cyt

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

1000000000 Cells/vial
Components
5 mL Tubes
20 x 1 Vial
BACS Streptavidin Microbubbles
1 x 15.5 mL
Human CD4+ T Cell Biotin Antibody Cocktail
1 x 1050 µL
Separation Buffer
1 x 200 mL

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Human CD4+ T Cell Isolation Kit delivers a highly enriched population of healthy, viable CD4+ T cells using a protocol that is fast, easy, and exceptionally gentle on delicate immune cells. 

Key facts

Assay time

30m

Assay type

Cell-based (quantitative)

Reactive species

Human

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

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Human CD4+ T Cell Isolation Kit delivers a highly enriched population of healthy, viable CD4+ T cells using a protocol that is fast, easy, and exceptionally gentle on delicate immune cells. 

  • This kit is designed to process 10 samples, up to 100 million cells per sample
  • Fast & easy workflow enriches human peripheral blood mononuclear cells (PBMCs) for CD4+ T cells in 30 minutes
  • No magnet, no column, no harsh chemicals
  • Microbubble enrichment maintains cell health and physiology

Mechanism of Action:Human CD4+ T Cell Isolation Kit uses a negative selection method to remove unwanted cell populations from the sample, effectively enriching for the T cells of interest while maintaining cellular physiology. The undesired (non-CD4+ T) cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD8, CD11b, CD14, CD16, CD19, CD36, CD56, CD123, CD235a, and TCR-γδ.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - Human CD4+ T Cell Isolation Kit (ab322033), expandable thumbnail

    Flow Cytometry - Human CD4+ T Cell Isolation Kit (ab322033)

    Untouched human CD4+ T cells were isolated from MNCs using the Human CD4+ T Cell Isolation Kit. Isolated cells were labeled with CD3-FITC and CD4-APC. The fluorescently labeled cells were analyzed by flow cytometry. Dead cells/debris were excluded from analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com