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AB322032

Human T Cell Isolation Kit

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Human T Cell Isolation Kit delivers a highly enriched population of healthy, viable T cells using a protocol that is fast, easy, and exceptionally gentle on delicate immune cells.
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Flow Cytometry - Human T Cell Isolation Kit (AB322032)
  • Flow Cyt

Supplier Data

Flow Cytometry - Human T Cell Isolation Kit (AB322032)

Untouched human T cells were isolated from MNCs using the Human T Cell Isolation Kit. Isolated cells were labeled with CD19-APC/Fire750 and CD3-FITC. The fluorescently labeled cells were analyzed by flow cytometry. Dead cells/debris were excluded from analysis.

Key facts

Assay time

30m

Assay type

Cell-based

Results type

Quantitative

Reacts with

Human

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Powered by Akadeum™

Human T Cell Isolation Kit delivers a highly enriched population of healthy, viable T cells using a protocol that is fast, easy, and exceptionally gentle on delicate immune cells.

  • This kit is designed to process 10 samples, up to 100 million cells per sample
  • Fast & easy workflow enriches human peripheral blood mononuclear cells (PBMCs) for T cells in 30 minutes
  • No magnet, no column, no harsh chemicals
  • Microbubble enrichment maintains cell health and physiology

Mechanism of Action: Human T Cell Isolation Kit uses a negative selection method to remove unwanted cell populations from the sample, effectively enriching for the T cells of interest while maintaining cellular physiology. The undesired (non-T) cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD14, CD16, CD19, CD36, CD56, CD123, and CD235a.

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What's included?

{ "values": { "1000000000Cellsvial": { "sellingSize": "1000000000 Cells/vial", "publicAssetCode":"ab322032-1000000000Cells", "assetComponentDetails": [ { "size":"1 x 1050 µL", "name":"Human T Cell Biotin Antibody Cocktail", "number":"AB322032-CMP02", "productcode":"" }, { "size":"20 x 1 Vial", "name":"5 mL Tubes", "number":"AB322032-CMP04", "productcode":"" }, { "size":"1 x 10.5 mL", "name":"BACS Streptavidin Microbubbles", "number":"AB322032-CMP01", "productcode":"" }, { "size":"1 x 200 mL", "name":"Separation Buffer", "number":"AB322032-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information T Cell
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com