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AB324125

Live Cell Tubulin Staining Kit (Fluorimetric)

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Live Cell Tubulin Staining Kit (Fluorimetric) (ab324125) provides a robust method for the fluorescent visualization of tubulins in live cells using Tubulite™ Deep Red.
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Functional Studies - Live Cell Tubulin Staining Kit (Fluorimetric) (AB324125)
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Supplier Data

Functional Studies - Live Cell Tubulin Staining Kit (Fluorimetric) (AB324125)

Imaging of Tubulin in live HeLa Cells : HeLa cells were co-labeled with Live Cell Tubulin Staining Kit (Fluorimetric) and incubated for 60 minutes at 37°C in a 5% CO₂ atmosphere. Fluorescence imaging was performed using a fluorescence microscope equipped with a Cy5 filter set.

Key facts

Detection method

Colorimetric/Fluorometric

Assay type

Cell-based

Product details

Live Cell Tubulin Staining Kit (Fluorimetric) (ab324125) provides a robust method for the fluorescent visualization of tubulins in live cells using Tubulite™ Deep Red. This cell-permeable probe enables live-cell imaging of tubulin dynamics without requiring fixation, facilitating real-time monitoring of tubulin polymerization. The deep red fluorescence of Tubulite™ Deep Red and efficient cellular permeability allows for multiplexing with other fluorescent labels, including GFP and nuclear stains such as DAPI. Once Tubulite™ Deep Red traverses the plasma membrane, cellular esterases hydrolyze the lipophilic blocking group, yielding a charged, membrane-impermeant product that remains well-retained within the cell, enabling sustained and stable imaging of intracellular tubulin structures.

What's included?

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

websiteProtocolBooklet
en

Product promise

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