Live Cell Tubulin Staining Kit (Fluorimetric) (ab324125) provides a robust method for the fluorescent visualization of tubulins in live cells using Tubulite™ Deep Red.
Images
Key facts
- Detection method
- Colorimetric/Fluorometric
- Assay type
- Cell-based
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
What's included?
Live Cell Tubulin Staining Kit (Fluorimetric) (ab324125) provides a robust method for the fluorescent visualization of tubulins in live cells using Tubulite™ Deep Red.
Key facts
- Detection method
- Colorimetric/Fluorometric
- Assay type
- Cell-based
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Live Cell Tubulin Staining Kit (Fluorimetric) (ab324125) provides a robust method for the fluorescent visualization of tubulins in live cells using Tubulite™ Deep Red. This cell-permeable probe enables live-cell imaging of tubulin dynamics without requiring fixation, facilitating real-time monitoring of tubulin polymerization. The deep red fluorescence of Tubulite™ Deep Red and efficient cellular permeability allows for multiplexing with other fluorescent labels, including GFP and nuclear stains such as DAPI. Once Tubulite™ Deep Red traverses the plasma membrane, cellular esterases hydrolyze the lipophilic blocking group, yielding a charged, membrane-impermeant product that remains well-retained within the cell, enabling sustained and stable imaging of intracellular tubulin structures.
Product promise
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Terms & Conditions.
1 product image
Functional Studies - Live Cell Tubulin Staining Kit (Fluorimetric) (ab324125)
Imaging of Tubulin in live HeLa Cells: HeLa cells were co-labeled with Live Cell Tubulin Staining Kit (Fluorimetric) and incubated for 60 minutes at 37°C in a 5% CO₂ atmosphere. Fluorescence imaging was performed using a fluorescence microscope equipped with a Cy5 filter set.
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