Microsome isolation kit

Microsome Isolation Kit (ab206995) provides a convenient and fast way to isolate microsomal fractions from animal tissues for downstream applications such as assessing CYP-mediated drug metabolism and xenobiotic biotransformation, and protein profiling of microsomal membrane proteins by SDS-PAGE and western blotting.

The microsome isolation kit enables preparation of active microsomes, without the need for ultracentrifugation or sucrose gradient fractionation. The kit contains sufficient reagents for 50 isolation procedures, yielding microsomes from roughly 25 grams of tissue or cultured cells.

Microsome isolation protocol summary:
- place tissue / washed cells in chilled Dounce homogenizer with homogenization buffer
- homogenize on ice and suspend in buffer
- vortex for 30 s and incubate on ice for 1 min
- spin at 10,000 g for 15 min
- discard floating lipid layer
- spin at 20,000 g for 20 min
- retain pellet
- wash pellet gently with buffer
- resuspend

This product is manufactured by BioVision, an Abcam company and was previously called K249 Microsome Isolation Kit. K249-50 is the same size as the 50 test size of ab206995.

Microsomes are spherical vesicle-like structures formed from membrane fragments following homogenization and fractionation of eukaryotic cells. The microsomal subcellular fraction is prepared by differential centrifugation and consists primarily of membranes derived from the endoplasmic reticulum (ER) and Golgi apparatus. Microsomes isolated from liver tissue are used extensively in pharmaceutical development, toxicology and environmental science to study the metabolism of drugs, organic pollutants and other xenobiotic compounds by the cytochrome P450 monooxidase (CYP) enzyme superfamily.