Microsome Isolation Kit (ab206995) provides a convenient and fast way to isolate microsomal fractions from animal tissues for downstream applications such as assessing CYP-mediated drug metabolism and xenobiotic biotransformation, and protein profiling of microsomal membrane proteins by SDS-PAGE and western blotting.
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- Scientific reports 13:118642023Evaluation and comparison of pharmacokinetic profiles and safety of two extended-release buprenorphine formulations in common marmosets (Callithrix jacchus).Applications:Unspecified applicationReactive species:Unspecified reactive speciesNiora J Fabian,Anthony J Mannion,Morgan Jamiel,David J Anderson,Joseph E Rower,Christopher A Reilly,William Menegas,Sureshkumar Muthupalani,Christina Ta,James G Fox,Robin Kramer,Jennifer L HauptPubMed 37481609
- Fundamental & clinical pharmacology 37:983-9932023Rapid high performance liquid chromatography method for erlotinib quantification in vitro: Application to study the effect of resveratrol on metabolism and cellular uptake of erlotinib.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAref Zayed,Jomana Al Hroot,Abdulraouf Mayyas,Belal Al-HuseinPubMed 37143385
- Journal of agricultural and food chemistry 70:14613-146212022Evidence for Cardiac Glycosides in Foliage of Colorado Potato Beetle-Resistant .Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanna J McCoy,Cuijuan Zeng,Emily McCoy,Pamela MacKinley,Jess Vickruck,Larry A Calhoun,Helen H TaiPubMed 36351172
- eLife 10:2021Characterization of the endogenous DAF-12 ligand and its use as an anthelmintic agent in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhu Wang,Mi Cheong Cheong,Jet Tsien,Heping Deng,Tian Qin,Jonathan Dc Stoltzfus,Tegegn G Jaleta,Xinshe Li,James B Lok,Steven A Kliewer,David J MangelsdorfPubMed 34874004
- iScience 24:1033112021GABA promotes the malignancy of human cancer cells by targeting the tyrosine phosphatase PTPN12.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Wei,Yini Zhu,Peng Yang,Yong Han,Suyun Wang,Xiaomei Wang,Shuai Xia,Xiaoguang Song,Zhongling Zhang,Sheng Wang,Philippe Rondard,Jean-Philippe Pin,Xinnong Jiang,Jianfeng LiuPubMed 34778730
- American journal of physiology. Lung cellular and 318:L1097-L11082020NADPH oxidase-mediated endothelial injury in HIV- and opioid-induced pulmonary arterial hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStuti Agarwal et. al.PubMed 32233792
- Journal of virology 93:2019Hepatitis B Virus Precore Protein p22 Inhibits Alpha Interferon Signaling by Blocking STAT Nuclear Translocation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBidisha Mitra,Jinyu Wang,Elena S Kim,Richeng Mao,Minhui Dong,Yuanjie Liu,Jiming Zhang,Haitao GuoPubMed 31019054
- Pesticide biochemistry and physiology 157:88-982019Vapor delivery of plant essential oils alters pyrethroid efficacy and detoxification enzyme activity in mosquitoes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesScott T O'Neal,Ellis J Johnson,Leslie C Rault,Troy D AndersonPubMed 31153481
- Journal of pharmaceutical and biomedical analysis 155:194-2012018LC-MS/MS method for the simultaneous quantification of intestinal CYP and UGT activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiana Busch et. al.PubMed 29649788
- Epilepsia 59:123-1342017Hepatic and hippocampal cytochrome P450 enzyme overexpression during spontaneous recurrent seizures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeonie Runtz,Benoit Girard,Marion Toussenot,Julie Espallergues,Alexis Fayd'Herbe De Maudave,Alexandre Milman,Frederic deBock,Chaitali Ghosh,Nathalie C Guérineau,Jean-Marc Pascussi,Federica Bertaso,Nicola MarchiPubMed 29125184
Key facts
- Assay time
- 45m
- Sample type
- Tissue, Suspension cells
What's included?
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Microsome Isolation Kit (ab206995) provides a convenient and fast way to isolate microsomal fractions from animal tissues for downstream applications such as assessing CYP-mediated drug metabolism and xenobiotic biotransformation, and protein profiling of microsomal membrane proteins by SDS-PAGE and western blotting.
Key facts
- Assay time
- 45m
- Sample type
- Tissue, Suspension cells
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Microsome Isolation Kit (ab206995) provides a convenient and fast way to isolate microsomal fractions from animal tissues for downstream applications such as assessing CYP-mediated drug metabolism and xenobiotic biotransformation, and protein profiling of microsomal membrane proteins by SDS-PAGE and western blotting.
The microsome isolation kit enables preparation of active microsomes, without the need for ultracentrifugation or sucrose gradient fractionation. The kit contains sufficient reagents for 50 isolation procedures, yielding microsomes from roughly 25 grams of tissue or cultured cells.
Microsome isolation protocol summary:
- place tissue / washed cells in chilled Dounce homogenizer with homogenization buffer
- homogenize on ice and suspend in buffer
- vortex for 30 s and incubate on ice for 1 min
- spin at 10,000 g for 15 min
- discard floating lipid layer
- spin at 20,000 g for 20 min
- retain pellet
- wash pellet gently with buffer
- resuspend
This product is manufactured by BioVision, an Abcam company and was previously called K249 Microsome Isolation Kit. K249-50 is the same size as the 50 test size of ab206995.
Microsomes are spherical vesicle-like structures formed from membrane fragments following homogenization and fractionation of eukaryotic cells. The microsomal subcellular fraction is prepared by differential centrifugation and consists primarily of membranes derived from the endoplasmic reticulum (ER) and Golgi apparatus. Microsomes isolated from liver tissue are used extensively in pharmaceutical development, toxicology and environmental science to study the metabolism of drugs, organic pollutants and other xenobiotic compounds by the cytochrome P450 monooxidase (CYP) enzyme superfamily.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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2 product images
Purification - Microsome isolation kit (ab206995)
Relative densitometry data demonstrate the enrichment of cytochrome P450 and reduction of mitochondrial protein marker in the microsomal fraction in comparison to whole rat liver homogenate (each column shows mean density ± SEM relative to whole liver lysate for at least 2 repeats).
Purification - Microsome isolation kit (ab206995)
Western blot analysis of microsomal and S9 fractions isolated from rat liver. Microsomes and S9 fraction were isolated according to the kit protocol described above. A total of 30 μg of protein in SDS-PAGE buffer was loaded in each lane and run on a 4-20% gradient gel. The blots were probed for cytochrome P450 (CYP2E1), the mitochondrial marker VDAC1 and the ER-specific protein marker Calnexin. Blots show enrichment of CYP2E1 and calnexin and depletion of mitochondrial membrane proteins in the microsomal fraction.
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