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AB65320

Mitochondria/Cytosol Fractionation Kit

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(136 Publications)

Mitochondria/cytosol fractionation kit (ab65320) provides a unique formulation of reagents for the effective isolation of highly enriched mitochondrial fractions from the cytosolic fraction of mammalian cells, including both apoptotic and non-apoptotic cells.

- Proven performance: cited in over 135 publications
- Available in different sizes to fit your experimental needs
- Compatible with our Lab essentials western blot workflow offering

Key facts

Assay time

4h

Sample types

Suspension cells, Adherent cells

Assay type

Direct

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Product details

Abcam's Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.

Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called K256 Mitochondria/Cytosol Fractionation Kit. K256-100 is the same size as the 100 test size of ab65320.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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Publications (136)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed40608429

2025

Cardiomyocyte cytosolic nuclear self-DNA contributes to the pathogenesis of desmoplakin cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyue Wang,Benjamin Cathcart,Quoc D Nguyen,Loi Q Lao,Amelia Bryans,Sara E Coleman,Leila Rouhi,Priyatansh Gurha,Ali J Marian

ACS omega 10:24018-24029 PubMed40547630

2025

Klotho Suppresses Indoxyl Sulfate-Mediated Apoptosis in Human Kidney Proximal Tubular (HK-2) Cells through Modulating the AKT/Nrf2 Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Chien-Yao Sun,Yao-Tsung Lin,Yu-Ting Huang,Hui-Ching Cheng,Wan-Ching Chou,Yu-Tzu Chang,Tsai-Chieh Ling,Pei-Ling Hsieh,Kun-Ling Tsai

PloS one 20:e0322962 PubMed40392781

2025

ABT-263, a BCL-2 inhibitor, selectively eliminates latently HIV-1-infected cells without viral reactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Jeong Eun Kang,Hyun Wook Seo,Dong-Eun Kim,Young Hyun Shin,Songmee Bae,Cheol-Hee Yoon

Journal of experimental & clinical cancer research : CR 44:149 PubMed40382627

2025

Novel Pt@PCN-Cu-induced cuproptosis amplifies αPD-L1 immunotherapy in pancreatic ductal adenocarcinoma through mitochondrial HK2-mediated PD-L1 upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Pengyu Wang,Weihua Guo,Shuyue Liu,Shouyi Li,Jiaqi Li,Bowen Ding,Fengyi Yin,Yang Yang,Xingjiang Li,Pei Cao,Chaozhe Ma,Wanying Zhang,Yidan Song,Yating Geng,Lantao Liu,Jing Hu,Jihui Hao,Yukuan Feng

iScience 28:112391 PubMed40322081

2025

Hyperandrogenism triggers mtDNA release to participate in ovarian inflammation via mPTP/cGAS/STING in PCOS.

Applications

Unspecified application

Species

Unspecified reactive species

Jieyu Cai,Qi Zhu,Yu Xiang,Linglin Weng,Na Liang,Xiaoqi Hong,Mingrui Xue,Hongshan Ge

Molecular neurobiology 62:10333-10348 PubMed40198445

2025

Epigenetics and Mitochondrial Biogenesis: The Role of Sirtuins in HIV Neuropathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

James Haorah,Hemavathi Iyappan,Malaroviyam Samikkannu,Karthick Chennakesavan,Jay P McLaughlin,Thangavel Samikkannu

Nature communications 16:2597 PubMed40090955

2025

Ubiquinol-mediated suppression of mitochondria-associated ferroptosis is a targetable function of lactate dehydrogenase B in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haibin Deng,Liang Zhao,Huixiang Ge,Yanyun Gao,Yan Fu,Yantang Lin,Mojgan Masoodi,Tereza Losmanova,Michaela Medová,Julien Ott,Min Su,Wenxiang Wang,Ren-Wang Peng,Patrick Dorn,Thomas Michael Marti

International journal of molecular sciences 26: PubMed40076489

2025

Integrin-Linked Kinase (ILK) Promotes Mitochondrial Dysfunction by Decreasing CPT1A Expression in a Folic Acid-Based Model of Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Mariano de la Serna-Soto,Laura Calleros,María Martos-Elvira,Ariadna Moreno-Piedra,Sergio García-Villoria,Mercedes Griera,Elena Alcalde-Estévez,Ana Asenjo-Bueno,Diego Rodríguez-Puyol,Sergio de Frutos,María Piedad Ruiz-Torres

Cells 14: PubMed40072058

2025

Tomatine Improves Glucose Metabolism and Mitochondrial Respiration in Insulin-Resistant Hepatocyte Cell Lines AML12 and HepG2 via an AMP-Activated Protein Kinase-Dependent Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Geon Lee,Donghwan Kim

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70377 PubMed39985305

2025

Dolutegravir induces endoplasmic reticulum stress at the blood-brain barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Huang,Qing Rui Qu,Md Tozammel Hoque,Reina Bendayan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com