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Mitochondria/cytosol fractionation kit provides a unique formulation of reagents for the effective isolation of highly enriched mitochondrial fractions from the cytosolic fraction of mammalian cells, including both apoptotic and non-apoptotic cells.


- Cited in over 110 publications
- Isolates mitochondrial and cytosolic fractions for studying apoptotic pathways and signal transduction
- Simple and easy procedure for fractionation
- Suitable for detecting translocation of factors between fractions via Western blotting (WB), ELISA, or other assays
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Key facts

Assay time
4h
Sample type
Suspension cells, Adherent cells
Assay type
Direct

What's included?

100 Test
Components
5X Cytosol Extraction Buffer I
1 x 20 mL
DTT II
1 x 100 µL
Mitochondria Extraction Buffer I
1 x 10 mL
Protease Inhibitor Cocktail I
1 x 1 Vial

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Mitochondria/cytosol fractionation kit provides a unique formulation of reagents for the effective isolation of highly enriched mitochondrial fractions from the cytosolic fraction of mammalian cells, including both apoptotic and non-apoptotic cells.


- Cited in over 110 publications
- Isolates mitochondrial and cytosolic fractions for studying apoptotic pathways and signal transduction
- Simple and easy procedure for fractionation
- Suitable for detecting translocation of factors between fractions via Western blotting (WB), ELISA, or other assays

Key facts

Assay time
4h
Sample type
Suspension cells, Adherent cells
Assay type
Direct

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam's Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.
Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called K256 Mitochondria/Cytosol Fractionation Kit. K256-100 is the same size as the 100 test size of ab65320.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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