Mitochondria isolation kit for cultured cells is designed for efficient isolation of mitochondria from cultured mammalian cells. The kit provides enough reagents for up to 20 isolations and is suitable for analyzing mitochondria by Western blotting, immunocapture, or activity assays.
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Mitochondria isolation kit for cultured cells is designed for efficient isolation of mitochondria from cultured mammalian cells. The kit provides enough reagents for up to 20 isolations and is suitable for analyzing mitochondria by Western blotting, immunocapture, or activity assays.
ab110170 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Available with a dounce homogenizer as kit Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) ab110171.
Mitochondrial isolation protocol summary:
- suspend cells in reagent A and incubate for 10 min
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant, repeat
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in reagent C and analyze or freeze
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Full details and terms and conditions can be found here:
Terms & Conditions.
Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110170 (MS852) Mitochondria Isolation Kit for Cultured Cells were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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