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ab110170 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells.

Assay time

1h

Reactive species

Mammals

Images

What's included?

1 Kit
Components
Reagent A
1 x 50 mL
Reagent B
1 x 50 mL
Reagent C
1 x 10 mL

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ab110170 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells.

Key facts

Assay time

1h

Reactive species

Mammals

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

ab110170 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

Available with a dounce homogenizer as kit ab110171.

Mitochondrial isolation protocol summary:
- suspend cells in reagent A and incubate for 10 min
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant, repeat
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in reagent C and analyze or freeze

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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