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Mitochondria isolation kit for cultured cells is designed for efficient isolation of mitochondria from cultured mammalian cells. The kit provides enough reagents for up to 20 isolations and is suitable for analyzing mitochondria by Western blotting, immunocapture, or activity assays.


- Cited in over 75 publications
- Isolates mitochondria from cultured mammalian cells with ease
- Useful in Western blotting, immunocapture, or mitochondrial activity assays
- Sufficient reagents for up to 20 benchtop mitochondria isolations

Images

Western blot - Mitochondria Isolation Kit for Cultured Cells (AB110170), expandable thumbnail

Publications

Key facts

Assay time
1h
Reactive species
Mammals

Associated Products

Select an associated product type

3 products for Alternative Product

What's included?

1 Kit
Components
Reagent A
1 x 50 mL
Reagent B
1 x 50 mL
Reagent C
1 x 10 mL

Recommended products

Mitochondria isolation kit for cultured cells is designed for efficient isolation of mitochondria from cultured mammalian cells. The kit provides enough reagents for up to 20 isolations and is suitable for analyzing mitochondria by Western blotting, immunocapture, or activity assays.


- Cited in over 75 publications
- Isolates mitochondria from cultured mammalian cells with ease
- Useful in Western blotting, immunocapture, or mitochondrial activity assays
- Sufficient reagents for up to 20 benchtop mitochondria isolations

Key facts

Assay time
1h
Reactive species
Mammals

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

ab110170 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

Available with a dounce homogenizer as kit Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) ab110171.

Mitochondrial isolation protocol summary:
- suspend cells in reagent A and incubate for 10 min
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant, repeat
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in reagent C and analyze or freeze

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Mitochondria Isolation Kit for Cultured Cells (ab110170), expandable thumbnail

    Western blot - Mitochondria Isolation Kit for Cultured Cells (ab110170)

    Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110170 (MS852) Mitochondria Isolation Kit for Cultured Cells were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com