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AB110171

Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer)

5

(1 Review)

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(34 Publications)

Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (ab110171) is designed for efficient isolation of mitochondria from cultured mammalian cells using differential centrifugation. Suitable for Western blot, immunocapture or activity assays.

- Provides reagents for up to 20 isolations
- Proven performance: cited in over 25 publications
1 Images
Western blot - Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (AB110171)
  • WB

Supplier Data

Western blot - Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (AB110171)

Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110171/MS853 Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.

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Key facts

Results type

Quantitative

Reacts with

Mammals

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Product details

ab110171 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab110171-1Kit", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Dounce Homogenizer (2 ml size) with pestles", "number":"AB110171-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"Reagent B", "number":"AB110171-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"Reagent C", "number":"AB110171-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Reagent A", "number":"AB110171-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

The AAPS journal 27:68 PubMed40140196

2025

Nomlabofusp, a Fusion Protein of Human Frataxin and a Cell Penetrant Peptide, Delivers Mature and Functional Frataxin into Mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew G Baile,John Jones,Natasha Sahr,Gopi Shankar

Cell death & disease 16:40 PubMed39863602

2025

Ferroptosis triggers mitochondrial fragmentation via Drp1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Lohans Pedrera,Laura Prieto Clemente,Alina Dahlhaus,Sara Lotfipour Nasudivar,Sofya Tishina,Daniel Olmo González,Jenny Stroh,Fatma Isil Yapici,Randhwaj Pratap Singh,Nils Grotehans,Thomas Langer,Ana J García-Sáez,Silvia von Karstedt

Current issues in molecular biology 47: PubMed39852159

2025

Notoginsenoside R1 Attenuates H/R Injury in H9c2 Cells by Maintaining Mitochondrial Homeostasis.

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Unspecified application

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Unspecified reactive species

Yuanbo Xu,Piao Wang,Ting Hu,Ke Ning,Yimin Bao

Nature communications 15:5818 PubMed38987265

2024

Compartmentalized mitochondrial ferroptosis converges with optineurin-mediated mitophagy to impact airway epithelial cell phenotypes and asthma outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuhiro Yamada,Claudette St Croix,Donna B Stolz,Yulia Y Tyurina,Vladimir A Tyurin,Laura R Bradley,Alexander A Kapralov,Yanhan Deng,Xiuxia Zhou,Qi Wei,Bo Liao,Nobuhiko Fukuda,Mara Sullivan,John Trudeau,Anuradha Ray,Valerian E Kagan,Jinming Zhao,Sally E Wenzel

Science advances 10:eadk9681 PubMed38820148

2024

HSDL2 links nutritional cues to bile acid and cholesterol homeostasis.

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Unspecified application

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Unspecified reactive species

Nolwenn Samson,Cristina R Bosoi,Christian Roy,Laurie Turcotte,Laura Tribouillard,Mathilde Mouchiroud,Line Berthiaume,Jocelyn Trottier,Heitor C G Silva,Thomas Guerbette,Ana Belén Plata-Gómez,Aurèle Besse-Patin,Alicia Montoni,Nicolò Ilacqua,Jennifer Lamothe,Yemima R Citron,Yves Gélinas,Stéphane Gobeil,Roberto Zoncu,Alexandre Caron,Mathieu Morissette,Luca Pellegrini,Patrick J Rochette,Jennifer L Estall,Alejo Efeyan,Michael Shum,Étienne Audet-Walsh,Olivier Barbier,André Marette,Mathieu Laplante

Nature communications 15:1203 PubMed38331987

2024

Targeting DNA2 overcomes metabolic reprogramming in multiple myeloma.

Applications

Unspecified application

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Unspecified reactive species

Natthakan Thongon,Feiyang Ma,Natalia Baran,Pamela Lockyer,Jintan Liu,Christopher Jackson,Ashley Rose,Ken Furudate,Bethany Wildeman,Matteo Marchesini,Valentina Marchica,Paola Storti,Giannalisa Todaro,Irene Ganan-Gomez,Vera Adema,Juan Jose Rodriguez-Sevilla,Yun Qing,Min Jin Ha,Rodrigo Fonseca,Caleb Stein,Caleb Class,Lin Tan,Sergio Attanasio,Guillermo Garcia-Manero,Nicola Giuliani,David Berrios Nolasco,Andrea Santoni,Claudio Cerchione,Carlos Bueso-Ramos,Marina Konopleva,Philip Lorenzi,Koichi Takahashi,Elisabet Manasanch,Gabriella Sammarelli,Rashmi Kanagal-Shamanna,Andrea Viale,Marta Chesi,Simona Colla

Aging cell 22:e13954 PubMed37614052

2023

Alterations of lipid-mediated mitophagy result in aging-dependent sensorimotor defects.

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Unspecified application

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Unspecified reactive species

Natalia Oleinik,Onder Albayram,Mohamed Faisal Kassir,F Cansu Atilgan,Chase Walton,Eda Karakaya,John Kurtz,Alexander Alekseyenko,Habeeb Alsudani,Megan Sheridan,Zdzislaw M Szulc,Besim Ogretmen

Cellular and molecular life sciences : CMLS 80:258 PubMed37594630

2023

Protease-independent control of parthanatos by HtrA2/Omi.

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Unspecified application

Species

Unspecified reactive species

Jonas Weiß,Michelle Heib,Thiemo Korn,Justus Hoyer,Johaiber Fuchslocher Chico,Susann Voigt,Tomas Koudelka,Andreas Tholey,Dieter Adam

International journal of molecular sciences 24: PubMed37373543

2023

Cholesterol, Amyloid Beta, Fructose, and LPS Influence ROS and ATP Concentrations and the Phagocytic Capacity of HMC3 Human Microglia Cell Line.

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Unspecified application

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Unspecified reactive species

Oscar M Muñoz Herrera,Brian V Hong,Ulises Ruiz Mendiola,Izumi Maezawa,Lee-Way Jin,Carlito B Lebrilla,Danielle J Harvey,Angela M Zivkovic

Nature immunology 24:991-1006 PubMed37095377

2023

Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yavuz F Yazicioglu,Eros Marin,Ciaran Sandhu,Silvia Galiani,Iwan G A Raza,Mohammad Ali,Barbara Kronsteiner,Ewoud B Compeer,Moustafa Attar,Susanna J Dunachie,Michael L Dustin,Alexander J Clarke
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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