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ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues.

Assay time

1h

Sample type

Tissue

Images

What's included?

1 Kit
Components
Isolation Buffer
1 x 100 mL
Wash Buffer
1 x 30 mL

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ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues.

Key facts

Assay time

1h

Sample type

Tissue

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

Mitochondrial isolation protocol summary:
- suspend minced tissue in isolation buffer
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in isolation buffer, spin at 12,000g for 15 min, collect pellet and repeat spin
- resuspend pellet in isolation buffer and analyze or freeze

Tested tissues: brain, liver and heart

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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