Mitochondria isolation kit for tissue is designed for the efficient isolation of mitochondria from hard or soft tissues, providing high-quality mitochondria for downstream analysis. The kit contains enough reagents for up to 10 isolations.
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Mitochondria isolation kit for tissue is designed for the efficient isolation of mitochondria from hard or soft tissues, providing high-quality mitochondria for downstream analysis. The kit contains enough reagents for up to 10 isolations.
ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Mitochondrial isolation protocol summary:
- suspend minced tissue in isolation buffer
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in isolation buffer, spin at 12,000g for 15 min, collect pellet and repeat spin
- resuspend pellet in isolation buffer and analyze or freeze
Tested tissues: brain, liver and heart
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Full details and terms and conditions can be found here:
Terms & Conditions.
Rat liver mitochondria were isolated using Abcam's Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer) ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 μg (lane 1) and 10 μg (lane 2) of mitochondria were tested with Abcam's Total OXPHOS Rodent WB Antibody Cocktail ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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