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Mitochondria isolation kit for tissue is designed for the efficient isolation of mitochondria from hard or soft tissues, providing high-quality mitochondria for downstream analysis. The kit contains enough reagents for up to 10 isolations.


- Cited in over 65 publications
- Isolates mitochondria from both hard and soft tissues, such as brain, liver, and heart
- Suitable for western blotting, immunocapture, or activity assays
- Simple Protocol – no need for ultracentrifugation, making the process more efficient and user-friendly

Images

Western blot - Mitochondria Isolation Kit for Tissue (AB110168), expandable thumbnail

Publications

Key facts

Assay time
1h
Sample type
Tissue

Associated Products

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3 products for Alternative Product

What's included?

1 Kit
Components
Isolation Buffer
1 x 100 mL
Wash Buffer
1 x 30 mL

Recommended products

Mitochondria isolation kit for tissue is designed for the efficient isolation of mitochondria from hard or soft tissues, providing high-quality mitochondria for downstream analysis. The kit contains enough reagents for up to 10 isolations.


- Cited in over 65 publications
- Isolates mitochondria from both hard and soft tissues, such as brain, liver, and heart
- Suitable for western blotting, immunocapture, or activity assays
- Simple Protocol – no need for ultracentrifugation, making the process more efficient and user-friendly

Key facts

Assay time
1h
Sample type
Tissue

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

Mitochondrial isolation protocol summary:
- suspend minced tissue in isolation buffer
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in isolation buffer, spin at 12,000g for 15 min, collect pellet and repeat spin
- resuspend pellet in isolation buffer and analyze or freeze

Tested tissues: brain, liver and heart

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Mitochondria Isolation Kit for Tissue (ab110168), expandable thumbnail

    Western blot - Mitochondria Isolation Kit for Tissue (ab110168)

    Rat liver mitochondria were isolated using Abcam's Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer) ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 μg (lane 1) and 10 μg (lane 2) of mitochondria were tested with Abcam's Total OXPHOS Rodent WB Antibody Cocktail ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com