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ab110169 (MS851) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues.

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Images

Western blot - Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer) (AB110169), expandable thumbnail

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Key facts

Sample type
Tissue
Assay type
Quantitative

Associated Products

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What's included?

1 Kit
Components
Dounce Homogenizer (2 ml size) with pestles
1 x 1 Unit
Isolation Buffer
1 x 100 mL
Wash Buffer
1 x 30 mL

Recommended products

ab110169 (MS851) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues.

Key facts

Sample type
Tissue
Assay type
Quantitative

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

ab110169 (MS851) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

Tested tissues: brain, liver and heart

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer) (ab110169), expandable thumbnail

    Western blot - Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer) (ab110169)

    Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 μg (lane 1) and 10 μg (lane 2) of mitochondria were tested with Abcam's Total OXPHOS Rodent WB Antibody Cocktail ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com