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AB322028

Mouse CD4+ T Cell Isolation Kit

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The Mouse CD4+ T Cell Isolation Kit was developed with BACS™ Microbubbles for the enrichment of untouched CD4+ T cells. Unwanted cells are depleted through negative selection.
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Flow Cytometry - Mouse CD4+ T Cell Isolation Kit (AB322028)
  • Flow Cyt

Supplier Data

Flow Cytometry - Mouse CD4+ T Cell Isolation Kit (AB322028)

Untouched murine CD4+ T cells were isolated from splenocytes using the Mouse CD4+ T Cell Isolation Kit. Isolated cells were labeled with CD3-APC and CD4-APC/Fire750. The fluorescently labeled cells were analyzed by flow cytometry. Debris and dead cells were excluded from analysis.

Key facts

Assay time

30m

Assay type

Cell-based (quantitative)

Reacts with

Mouse

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Powered by Akadeum™

The Mouse CD4+ T Cell Isolation Kit was developed with BACS™ Microbubbles for the enrichment of untouched CD4+ T cells. Unwanted cells are depleted through negative selection.

  • This kit can process up to 1 billion cells
  • Fast & easy workflow enriches splenocytes for CD4+ T cells in 30 minutes
  • No magnet, no column, no harsh chemicals
  • Microbubble enrichment maintains cell health and physiology

Mechanism of Action:The Mouse CD4+ T Cell Isolation Kit uses a negative selection method to remove unwanted cell populations and effectively enrich CD4+ T cells. Unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD8a, CD11b, CD11c, CD19, CD24, CD49b, CD105, Gr1, Ter119, TCRγδ.

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What's included?

{ "values": { "1000000000Cellsvial": { "sellingSize": "1000000000 Cells/vial", "publicAssetCode":"ab322028-1000000000Cells", "assetComponentDetails": [ { "size":"1 x 1050 µL", "name":"Mouse CD4+ T Cell Biotin Antibody Cocktail", "number":"AB322028-CMP02", "productcode":"" }, { "size":"20 x 1 Vial", "name":"5 mL Tubes", "number":"AB322028-CMP04", "productcode":"" }, { "size":"1 x 15.5 mL", "name":"BACS Streptavidin Microbubbles", "number":"AB322028-CMP01", "productcode":"" }, { "size":"1 x 200 mL", "name":"Separation Buffer", "number":"AB322028-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information CD4+ T Cell
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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