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The Mouse Naïve CD4+ T Cell Isolation Kit was developed with BACS™ Microbubbles for the enrichment of untouched Naïve CD4+ T cells. Unwanted cells are depleted through negative selection.

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Images

Flow Cytometry - Mouse Naive CD4+ T Cell Isolation Kit (AB322029), expandable thumbnail

Key facts

Assay time

30m

Assay type

Cell-based (quantitative)

Reactive species

Mouse

Reactivity data

Application

Flow Cyt

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

1000000000 Cells/vial
Components
5 mL Tubes
20 x 1 Vial
BACS Streptavidin Microbubbles
1 x 10.5 mL
Mouse Naive CD4+ T Cell Biotin Antibody Cocktail
1 x 1050 µL
Separation Buffer
1 x 200 mL

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The Mouse Naïve CD4+ T Cell Isolation Kit was developed with BACS™ Microbubbles for the enrichment of untouched Naïve CD4+ T cells. Unwanted cells are depleted through negative selection.

Key facts

Assay time

30m

Assay type

Cell-based (quantitative)

Reactive species

Mouse

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

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The Mouse Naïve CD4+ T Cell Isolation Kit was developed with BACS™ Microbubbles for the enrichment of untouched Naïve CD4+ T cells. Unwanted cells are depleted through negative selection.

  • This kit can process up to 1 billion cells
  • Fast & easy workflow enriches splenocytes for Naïve CD4+ T cells in 30 minutes
  • No magnet, no column, no harsh chemicals
  • Microbubble enrichment maintains cell health and physiology

Mechanism of Action:The Mouse Naïve CD4+ T Cell Isolation Kit uses a negative selection method to remove unwanted cell populations and effectively enrich Naïve CD4+ T cells. Unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD8a, CD11b, CD11c, CD19, CD24, CD25, CD44, CD49b, CD105, Gr1, Ter119, TCRγδ.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - Mouse Naive CD4+ T Cell Isolation Kit (ab322029), expandable thumbnail

    Flow Cytometry - Mouse Naive CD4+ T Cell Isolation Kit (ab322029)

    Untouched murine Naïve CD4+ T cells were isolated from splenocytes using the Mouse Naïve CD4+ T cell Isolation Kit. Isolated cells were labeled with CD62L-PerCPCy5.5 and CD4-APC/Fire750. The fluorescently labeled cells were analyzed by flow cytometry. Debris and dead cells were excluded from analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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