Nuclear Extraction Kit

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Functional Studies - Nuclear Extraction Kit (AB113474)

Nuclear extracts were prepared from MCF-7 cells and the activity of HDACs were measured using different amounts of the extract. The result shown in the figure demonstrates the ab113474's high specificity.

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Functional Studies - Nuclear Extraction Kit (AB113474)

Effect of two drugs refered to as Cilo (50 and 100 mg/kg; Cilo50and Cilo100), and Pio (3 and 10 mg/kg; Pio3 and Pio10), and their combination (Cilo50and Pio3) on the PPAR-γ transcription activity in rats subjected to ischemia (45 min)/reperfusion (24 hrs).
Drugs were administered orally for 14 days then subjected to ischemia/reperfusion. Values are expressed as mean ± S.E.M (n = 6). Data are compared with sham operated control (#), I/R control (∗), Cilo50 (), Pio3 (‡), and combination (§) pretreated groups (one-way ANOVA followed by Tukey Multiple Comparison Test) at P<0.05.

Ragab, Diaa et al., PloS one: vol. 9,5 e95313., Fig 6, doi:10.1371/journal.pone.0095313

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Functional Studies - Nuclear Extraction Kit (AB113474)

B16F10 cells were treated with 30 µM of DMPB for the indicated time periods. Cytoplasmic and nuclear fractions were isolated and analyzed by Western blotting.

Park, Jisu et al., PloS one: vol. 10,11 e0141988., Fig 4, doi:10.1371/journal.pone.0141988