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Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477) provides a simple and selective method for extracting nuclear proteins (nucleic-acid free) in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, or any other procedures requiring optimized nucleic acid-free nuclear proteins.

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Key facts

Assay time
1h
Sample type
Tissue, Suspension cells, Adherent cells
Reactive species
Mammals

Associated Products

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What's included?

100 Test
Components
1000X DTT Solution
1 x 100 µL
1000X Protease Inhibitor Cocktail (PIC)
1 x 100 µL
10X Pre-Extraction Buffer
1 x 10 mL
Extraction Buffer
1 x 10 mL
Extraction Cleaner
1 x 100 µL
Extraction Pre-Cleaner
1 x 1 mL

Recommended products

Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477) provides a simple and selective method for extracting nuclear proteins (nucleic-acid free) in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, or any other procedures requiring optimized nucleic acid-free nuclear proteins.

Key facts

Assay time
1h
Sample type
Tissue, Suspension cells, Adherent cells
Reactive species
Mammals

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477) provides a simple and selective method for extracting nuclear proteins (nucleic-acid free) in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, or any other procedures requiring optimized nucleic acid-free nuclear proteins.

ab113477 can be used to extract nuclear proteins from mammalian cells and tissue samples free of nucleic acids attached.

Not sure if this is the right product for you? Check out our Methods and tools to study histone modifications for help.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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