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AB102538

PEG Virus Precipitation Kit

5

(2 Reviews)

|

(26 Publications)

PEG Virus Precipitation Kit ab102538 provides an easy, convenient and time-saving method to concentrate viruses without ultra-centrifugation.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - PEG Virus Precipitation Kit (AB102538)
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Supplier Data

Functional Studies - PEG Virus Precipitation Kit (AB102538)

Concentration of baculovirus : Low titer baculovirus (10 ml) expressing human Granzyme B was precipitated following the kit protocol and the precipitate was suspended in 1 ml of Virus Re-suspension Solution. Both low titer and precipitated baculovirus was subsequently used to infect SF9 Insect cells. The activity of recombinant Granzyme B secreted into the culture medium by low titer and precipitated baculovirus infected insect cells was monitored using ab157403 - Granzyme B Activity Assay Kit (Fluorometric).

Key facts

Sample types

Cell culture media, Other biological fluids

Assay type

Direct

Range

40 - 100 %

Sensitivity

> 40 %

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Product details

PEG Virus Precipitation Kit ab102538 provides an easy, convenient and time-saving method to concentrate viruses without ultra-centrifugation.

Viruses are usually produced at a low titer. They often need to be concentrated for storage or further applications. A quick, easy and inexpensive method is desired to concentrate viruses and remove impurities.

The PEG Virus Precipitation method is suitable for both small scale lab samples, and large scale virus preparations with high yield and high viral titer. It can be used to concentrate viruses including retroviruses, baculoviruses, lentiviruses, and phage, in cell culture medium and environmental samples. Viruses can be concentrated over 100 fold. The concentrated virus can be used for infection, viral DNA or RNA purification, etc.

An optimized Virus Re-suspension Solution is included in the kit to maximize viral recovery by 40-100% depending on the virus type and sources. The whole process uses non-toxic reagents.

This product was previously called K904 Biovision PEG Virus Precipitation Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab102538-50Test", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Virus Resuspension Solution (1X)", "number":"AB102538-CMP02", "productcode":"" }, { "size":"1 x 125 mL", "name":"5X PEG Solution", "number":"AB102538-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information PEG
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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 43:107 PubMed38594748

2024

A phenotypic screening approach to target p60AmotL2-expressing invasive cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Fonseca,Weiyingqi Cui,Nona Struyf,Le Tong,Ayushi Chaurasiya,Felipe Casagrande,Honglei Zhao,Dinura Fernando,Xinsong Chen,Nicholas P Tobin,Brinton Seashore-Ludlow,Andreas Lundqvist,Johan Hartman,Anita Göndör,Päivi Östling,Lars Holmgren

American journal of physiology. Lung cellular and molecular physiology 326:L175-L189 PubMed38147795

2023

Does vaping increase the likelihood of SARS-CoV-2 infection? Paradoxically yes and no.

Applications

Unspecified application

Species

Unspecified reactive species

Rattapol Phandthong,Man Wong,Ann Song,Teresa Martinez,Prue Talbot

Journal of medical virology 95:e28952 PubMed37455550

2023

Synthetic gRNA/Cas9 ribonucleoprotein targeting HBV DNA inhibits viral replication.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyu Zhang,Yi Zhang,Sushant Khanal,Dechao Cao,Juan Zhao,Xindi Dang,Lam Ngoc Thao Nguyen,Madison Schank,Xiao Y Wu,Yong Jiang,Shunbin Ning,Ling Wang,Mohamed El Gazzar,Jonathan P Moorman,Haitao Guo,Zhi Q Yao

Nature communications 13:6993 PubMed36384944

2022

Ascertaining cells' synaptic connections and RNA expression simultaneously with barcoded rabies virus libraries.

Applications

Unspecified application

Species

Unspecified reactive species

Arpiar Saunders,Kee Wui Huang,Cassandra Vondrak,Christina Hughes,Karina Smolyar,Harsha Sen,Adrienne C Philson,James Nemesh,Alec Wysoker,Seva Kashin,Bernardo L Sabatini,Steven A McCarroll

The EMBO journal 41:e110372 PubMed36124865

2022

TSG101 associates with PARP1 and is essential for PARylation and DNA damage-induced NF-κB activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmet Buğra Tufan,Katina Lazarow,Marina Kolesnichenko,Anje Sporbert,Jens Peter von Kries,Claus Scheidereit

The Journal of international medical research 50:3000605221104760 PubMed35726595

2022

Potential role of HBV DNA-induced CD8 T cell apoptosis in patients with systemic lupus erythematosus and rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Zifeng Wang,Fen Wang,Haiyang Ma,Shujuan Lv

Bio-protocol 12:e4392 PubMed35800100

2022

Production of Recombinant Hepatitis B virus (HBV) and Detection of HBV in Infected Human Liver Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Tanvir Hossain,Shahla Romal,Tokameh Mahmoudi

Bioengineering (Basel, Switzerland) 9: PubMed35324780

2022

Engineering Novel Lentiviral Vectors for Labelling Tumour Cells and Oncogenic Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Seçkin Akgül,Carolin Offenhäuser,Anja Kordowski,Bryan W Day

Molecular therapy : the journal of the American Society of Gene Therapy 30:1979-1993 PubMed35167974

2022

Human inhalable antibody fragments neutralizing SARS-CoV-2 variants for COVID-19 therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Minenkova,Daniela Santapaola,Ferdinando Maria Milazzo,Anna Maria Anastasi,Gianfranco Battistuzzi,Caterina Chiapparino,Antonio Rosi,Giuseppe Gritti,Gianmaria Borleri,Alessandro Rambaldi,Clélia Dental,Cécile Viollet,Bruno Pagano,Laura Salvini,Emanuele Marra,Laura Luberto,Antonio Rossi,Anna Riccio,Emilio Merlo Pich,Maria Gabriella Santoro,Rita De Santis

Biotechnology journal 16:e2100176 PubMed34506679

2021

Surrogate model to screen for inactivation-based clearance of enveloped viruses during biotherapeutics process development.

Applications

Unspecified application

Species

Unspecified reactive species

Hasin Feroz,Daniel Cetnar,Robert Hewlett,Satish Sharma,Melissa Holstein,Sanchayita Ghose,Zheng Jian Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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