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Plasma membrane protein extraction kit provides optimized buffers and reagents for the effective extraction of plasma membrane proteins from mammalian tissues and cells. The kit offers the option to either extract total cellular membrane proteins or specifically purify plasma membrane proteins.


- Cited in over 200 publications
- High yield and purity – consistent yield with high purity (over 90%)
- Suitable for western blotting, 2-D gels, enzyme analyses, and more
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Images

Western blot - Plasma Membrane Protein Extraction Kit (AB65400), expandable thumbnail
  • Functional Studies - Plasma Membrane Protein Extraction Kit (AB65400), expandable thumbnail

Publications

Key facts

Assay time
1h
Sample type
Tissue Homogenate, Cell culture extracts

What's included?

50 Test
Components
Homogenization Buffer I
1 x 100 mL
Lower Phase Solution
1 x 20 mL
Protease Inhibitor Cocktail I
1 x 1 Vial
Upper Phase Solution
1 x 20 mL

Recommended products

Plasma membrane protein extraction kit provides optimized buffers and reagents for the effective extraction of plasma membrane proteins from mammalian tissues and cells. The kit offers the option to either extract total cellular membrane proteins or specifically purify plasma membrane proteins.


- Cited in over 200 publications
- High yield and purity – consistent yield with high purity (over 90%)
- Suitable for western blotting, 2-D gels, enzyme analyses, and more

Key facts

Assay time
1h
Sample type
Tissue Homogenate, Cell culture extracts

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Plasma Membrane Protein Extraction Kit ab65400 provides optimized buffers and reagents for effective extraction of plasma membrane proteins from mammalian tissues and cells.

Other commercially available methods can only extract the total cellular membrane proteins (combinations of plasma and organelle membrane proteins). The ab65400 kit offers two options: either extract the total cellular membrane proteins, or purify the plasma membrane proteins specifically.

The procedure offers consistent yield and high purity (over 90%). Membrane proteins prepared using the kit can be utilized in a variety of applications, such as Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.

This product is manufactured by BioVision, an Abcam company and was previously called K268 Membrane Protein Extraction Kit. K268-50 is the same size as the 50 test size of ab65400.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Plasma Membrane Protein Extraction Kit (ab65400), expandable thumbnail
    Image from Choveau F.S et al., PLoS One 10(12), Fig 2C. Doi: 10.1371/journal.pone.0145367. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Western blot - Plasma Membrane Protein Extraction Kit (ab65400)

    Cell membrane protein was collected using plasma membrane protein extraction kit (ab65400). The upper panels show the plasma membrane myc-tagged protein; the middle panels show the cytosolic myc-tagged proteins and the lower panels show the total myc-tagged protein from CHO (Chinese Hamster ovary) whole-cell lysates.

  • Functional Studies - Plasma Membrane Protein Extraction Kit (ab65400), expandable thumbnail
    Kweon, Hae-Jin et al., Scientific reports: vol. 6 30684., Fig 1, doi:10.1038/srep30684

    Functional Studies - Plasma Membrane Protein Extraction Kit (ab65400)

    Western blotting on the plasma membrane (PM) fraction extracted useing ab65400, total cellular membrane (TCM) fraction, and total lysate of cells expressing GFP-tagged ASIC2a or ASIC2b was performed using anti-GFP antibody. As controls, the PM and the TCM fractions were blotted using anti-calnexin (CNX) antibody, and total lysate was blotted using anti-GAPDH antibody.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com