Plasma membrane protein extraction kit provides optimized buffers and reagents for the effective extraction of plasma membrane proteins from mammalian tissues and cells. The kit offers the option to either extract total cellular membrane proteins or specifically purify plasma membrane proteins.
- Cited in over 200 publications
- High yield and purity – consistent yield with high purity (over 90%)
- Suitable for western blotting, 2-D gels, enzyme analyses, and more
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- Virology journal 21:72024The artificial amino acid change in the sialic acid-binding domain of the hemagglutinin neuraminidase of newcastle disease virus increases its specificity to HCT 116 colorectal cancer cells and tumor suppression effect.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo-Kyoung Jung,Yong Hee An,Sung Hoon Jang,Jin-Ju Jang,Seonhee Kim,Joo Hee Jeon,Jinju Kim,Jason Jungsik Song,Hyun JangPubMed 38178138
- Cell death & disease 14:7612023Targeting protein methylation in pancreatic cancer cells results in KRAS signaling imbalance and inhibition of autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría F Montenegro,Román Martí-Díaz,Ana Navarro,Jorge Tolivia,Luis Sánchez-Del-Campo,Juan Cabezas-Herrera,José Neptuno Rodríguez-LópezPubMed 37996408
- Nature communications 14:49892023Endothelial ERα promotes glucose tolerance by enhancing endothelial insulin transport to skeletal muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnastasia Sacharidou,Ken Chambliss,Jun Peng,Jose Barrera,Keiji Tanigaki,Katherine Luby-Phelps,İpek Özdemir,Sohaib Khan,Shashank R Sirsi,Sung Hoon Kim,Benita S Katzenellenbogen,John A Katzenellenbogen,Mohammed Kanchwala,Adwait A Sathe,Andrew Lemoff,Chao Xing,Kenneth Hoyt,Chieko Mineo,Philip W ShaulPubMed 37591837
- Phytotherapy research : PTR 37:3898-39122023Sclareol attenuates liver fibrosis through SENP1-mediated VEGFR2 SUMOylation and inhibition of downstream STAT3 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMao-Xu Ge,Wei-Xiao Niu,Yun-Yang Bao,Zhen-Ning Lu,Hong-Wei HePubMed 37132081
- Cancer research 83:2034-20512023ZDHHC2-Mediated AGK Palmitoylation Activates AKT-mTOR Signaling to Reduce Sunitinib Sensitivity in Renal Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Sun,Liang Zhu,Pian Liu,Huan Zhang,Feng Guo,Xin JinPubMed 37078777
- Nature communications 14:17852023Defective BVES-mediated feedback control of cAMP in muscular dystrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaiwen Li,Peipei Wang,Chen Zhang,Yuanbojiao Zuo,Yuan Zhou,Renzhi HanPubMed 36997581
- Journal of periodontal research 58:634-6452023Pre-conditioning of gingival epithelial cells with sub-apoptotic concentrations of curcumin prevents pro-inflammatory cytokine release.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelissa M Grant,Ann E Scott,John B Matthews,Helen R Griffiths,Iain L C ChapplePubMed 36919895
- Nature communications 14:8702023Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarsten Nalbach,Martina Schifferer,Debjani Bhattacharya,Hung Ho-Xuan,Wei Chou Tseng,Luis A Williams,Alexandra Stolz,Stefan F Lichtenthaler,Zvulun Elazar,Christian BehrendsPubMed 36797266
- Frontiers in immunology 14:10902022023Inhibition of sphingosine-1-phosphate receptor 3 suppresses ATP-induced NLRP3 inflammasome activation in macrophages TWIK2-mediated potassium efflux.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingqin Wang,Chen Wang,Qiaolan He,Guannan Chen,Jie Yu,Jing Cang,Ming ZhongPubMed 36798132
- Science translational medicine 14:eabj42212022A monoclonal antibody targeting nonjunctional claudin-1 inhibits fibrosis in patient-derived models by modulating cell plasticity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatascha Roehlen,Antonio Saviano,Houssein El Saghire,Emilie Crouchet,Zeina Nehme,Fabio Del Zompo,Frank Jühling,Marine A Oudot,Sarah C Durand,François H T Duong,Sara Cherradi,Victor Gonzalez Motos,Nuno Almeida,Clara Ponsolles,Laura Heydmann,Tessa Ostyn,Antonin Lallement,Patrick Pessaux,Emanuele Felli,Andrea Cavalli,Jacopo Sgrignani,Christine Thumann,Olga Koutsopoulos,Bryan C Fuchs,Yujin Hoshida,Maike Hofmann,Mogens Vyberg,Birgitte Martine Viuff,Elisabeth D Galsgaard,Greg Elson,Alberto Toso,Markus Meyer,Roberto Iacone,Tamas Schweighoffer,Geoffrey Teixeira,Solange Moll,Claudio De Vito,Tania Roskams,Irwin Davidson,Danijela Heide,Mathias Heikenwälder,Mirjam B Zeisel,Joachim Lupberger,Laurent Mailly,Catherine Schuster,Thomas F BaumertPubMed 36542691
Key facts
- Assay time
- 1h
- Sample type
- Tissue Homogenate, Cell culture extracts
What's included?
Recommended products
Plasma membrane protein extraction kit provides optimized buffers and reagents for the effective extraction of plasma membrane proteins from mammalian tissues and cells. The kit offers the option to either extract total cellular membrane proteins or specifically purify plasma membrane proteins.
- Cited in over 200 publications
- High yield and purity – consistent yield with high purity (over 90%)
- Suitable for western blotting, 2-D gels, enzyme analyses, and more
Key facts
- Assay time
- 1h
- Sample type
- Tissue Homogenate, Cell culture extracts
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Plasma Membrane Protein Extraction Kit ab65400 provides optimized buffers and reagents for effective extraction of plasma membrane proteins from mammalian tissues and cells.
Other commercially available methods can only extract the total cellular membrane proteins (combinations of plasma and organelle membrane proteins). The ab65400 kit offers two options: either extract the total cellular membrane proteins, or purify the plasma membrane proteins specifically.
The procedure offers consistent yield and high purity (over 90%). Membrane proteins prepared using the kit can be utilized in a variety of applications, such as Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.
This product is manufactured by BioVision, an Abcam company and was previously called K268 Membrane Protein Extraction Kit. K268-50 is the same size as the 50 test size of ab65400.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Image from Choveau F.S et al., PLoS One 10(12), Fig 2C. Doi: 10.1371/journal.pone.0145367. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Western blot - Plasma Membrane Protein Extraction Kit (ab65400)
Cell membrane protein was collected using plasma membrane protein extraction kit (ab65400). The upper panels show the plasma membrane myc-tagged protein; the middle panels show the cytosolic myc-tagged proteins and the lower panels show the total myc-tagged protein from CHO (Chinese Hamster ovary) whole-cell lysates.
Kweon, Hae-Jin et al., Scientific reports: vol. 6 30684., Fig 1, doi:10.1038/srep30684Functional Studies - Plasma Membrane Protein Extraction Kit (ab65400)
Western blotting on the plasma membrane (PM) fraction extracted useing ab65400, total cellular membrane (TCM) fraction, and total lysate of cells expressing GFP-tagged ASIC2a or ASIC2b was performed using anti-GFP antibody. As controls, the PM and the TCM fractions were blotted using anti-calnexin (CNX) antibody, and total lysate was blotted using anti-GAPDH antibody.
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