AB65400

Plasma Membrane Protein Extraction Kit

Name: Plasma membrane protein extraction kit, 50 tests (ab65400) | Abcam
Brand: Abcam Limited
SKU: AB65400
Price: 655 USD
Availability: InStock

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(223 Publications)

Plasma membrane protein extraction kit (ab65400) provides optimized buffers and reagents for the effective extraction of native plasma membrane proteins from mammalian tissues and cells. Suitable for downstream applications such as western bot and SDS-PAGE.

- Option to either extract total cellular membrane proteins or specifically purify plasma membrane proteins
- Compatible with our Lab essentials western blot workflow offering
- Proven performance: cited in over 220 publications

2 Images

PubMed

Western blot - Plasma Membrane Protein Extraction Kit (AB65400)

Cell membrane protein was collected using plasma membrane protein extraction kit (ab65400). The upper panels show the plasma membrane myc-tagged protein; the middle panels show the cytosolic myc-tagged proteins and the lower panels show the total myc-tagged protein from CHO (Chinese Hamster ovary) whole-cell lysates.

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Image from Choveau F.S et al., PLoS One 10(12), Fig 2C. Doi: 10.1371/journal.pone.0145367. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Functional Studies - Plasma Membrane Protein Extraction Kit (AB65400)

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Functional Studies - Plasma Membrane Protein Extraction Kit (AB65400)

Western blotting on the plasma membrane (PM) fraction extracted useing ab65400, total cellular membrane (TCM) fraction, and total lysate of cells expressing GFP-tagged ASIC2a or ASIC2b was performed using anti-GFP antibody. As controls, the PM and the TCM fractions were blotted using anti-calnexin (CNX) antibody, and total lysate was blotted using anti-GAPDH antibody.

Kweon, Hae-Jin et al., Scientific reports: vol. 6 30684., Fig 1, doi:10.1038/srep30684

Key facts

Assay time

Sample types

Tissue Homogenate, Cell culture extracts

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Product details

Plasma Membrane Protein Extraction Kit ab65400 provides optimized buffers and reagents for effective extraction of plasma membrane proteins from mammalian tissues and cells.

Other commercially available methods can only extract the total cellular membrane proteins (combinations of plasma and organelle membrane proteins). The ab65400 kit offers two options: either extract the total cellular membrane proteins, or purify the plasma membrane proteins specifically.

The procedure offers consistent yield and high purity (over 90%). Membrane proteins prepared using the kit can be utilized in a variety of applications, such as Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.

This product is manufactured by BioVision, an Abcam company and was previously called K268 Membrane Protein Extraction Kit. K268-50 is the same size as the 50 test size of ab65400.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab65400-50Test", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Upper Phase Solution", "number":"AB65400-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"Lower Phase Solution", "number":"AB65400-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Protease Inhibitor Cocktail I", "number":"AB65400-CMP03", "productcode":"" }, { "size":"1 x 100 mL", "name":"Homogenization Buffer I", "number":"AB65400-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

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Publications (223)

Recent publications for all applications. Explore the full list and refine your search

Genome research 35:1456-1471 PubMed40360186

2025

Charting the regulatory landscape of TP53 on transposable elements in cancer.

Applications

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Species

Unspecified reactive species

Xuan Qu,Yonghao Liang,Colin McCornack,Xiaoyun Xing,Heather Schmidt,Chad Tomlinson,Catrina Fronick,Edward A Belter,Juan F Macias-Velasco,Ting Wang

Science advances 11:eadr5703 PubMed40333988

2025

Niemann Pick C1 mistargeting disrupts lysosomal cholesterol homeostasis contributing to neurodegeneration in a Batten disease model.

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Abhilash P Appu,Maria B Bagh,Nisha Plavelil,Avisek Mondal,Tamal Sadhukhan,Satya P Singh,Neil J Perkins,Aiyi Liu,Anil B Mukherjee

Journal of cellular and molecular medicine 29:e70352 PubMed39855896

2025

Stromal Cell Derived Factor-1 Promotes Hepatic Insulin Resistance via Inhibiting Hepatocyte Lipophagy.

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Unspecified reactive species

Chunfeng Lu,Yuting Zhang,Cuilian Sun,Yuhang Na,Haotian Sun,Jianhua Ma,Xueqin Wang,Xiaomin Cang

International journal of molecular sciences 25: PubMed39519101

2024

Oligonol, an Oligomerized Polyphenol from , Enhances Branched-Chain Amino Acid Transportation and Catabolism to Alleviate Sarcopenia.

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Yun-Ching Chang,Yu-Chi Chen,Yin-Ching Chan,Cheng Liu,Sue-Joan Chang

Communications biology 7:1481 PubMed39521920

2024

Neurexin facilitates glycosylation of Dystroglycan to sustain muscle architecture and function in Drosophila.

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Yu Zhao,Junhua Geng,Zhu Meng,Yichen Sun,Mengzhu Ou,Lizhong Xu,Moyi Li,Guangming Gan,Menglong Rui,Junhai Han,Wei Xie

Molecular cancer 23:242 PubMed39478560

2024

Tumour-intrinsic PDL1 signals regulate the Chk2 DNA damage response in cancer cells and mediate resistance to Chk1 inhibitors.

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Clare E Murray,Anand V R Kornepati,Carlos Ontiveros,Yiji Liao,Bárbara de la Peña Avalos,Cody M Rogers,Zexuan Liu,Yilun Deng,Haiyan Bai,Suresh Kari,Alvaro S Padron,Jacob T Boyd,Ryan Reyes,Curtis A Clark,Robert S Svatek,Rong Li,Yanfen Hu,Meiling Wang,José R Conejo-Garcia,Lauren A Byers,Kavya Ramkumar,Anil K Sood,Jung-Min Lee,Christin E Burd,Ratna K Vadlamudi,Harshita B Gupta,Weixing Zhao,Eloïse Dray,Patrick Sung,Tyler J Curiel

PLoS pathogens 20:e1011777 PubMed38913740

2024

Characterization of Unique Pathological Features of COVID-Associated Coagulopathy: Studies with AC70 hACE2 Transgenic Mice Highly Permissive to SARS-CoV-2 Infection.

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Species

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Aleksandra K Drelich,Kempaiah Rayavara,Jason Hsu,Panatda Saenkham-Huntsinger,Barbara M Judy,Vivian Tat,Thomas G Ksiazek,Bi-Hung Peng,Chien-Te K Tseng

Proteome science 22:6 PubMed38750478

2024

iTRAQ-based proteomic study on monocyte cell model discovered an association of LAMP2 downregulation with HIV-1 latency.

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Lin Yin,Qimin Wang,Siyuan Liu,Jun Chen,Yujiao Zhang,Lingqing Lu,Hongzhou Lu,Zhigang Song,Lijun Zhang

Circulation research 134:e52-e71 PubMed38497220

2024

Extracellular Kir2.1 Mutant Upsets Kir2.1-PIP Bonds and Is Arrhythmogenic in Andersen-Tawil Syndrome.

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Species

Unspecified reactive species

Francisco M Cruz,Álvaro Macías,Ana I Moreno-Manuel,Lilian K Gutiérrez,María Linarejos Vera-Pedrosa,Isabel Martínez-Carrascoso,Patricia Sánchez Pérez,Juan Manuel Ruiz Robles,Francisco J Bermúdez-Jiménez,Aitor Díaz-Agustín,Fernando Martínez de Benito,Salvador Arias-Santiago,Aitana Braza-Boils,Mercedes Martín-Martínez,Marta Gutierrez-Rodríguez,Juan A Bernal,Esther Zorio,Juan Jiménez-Jaimez,José Jalife

Journal of biomedical science 31:20 PubMed38321486

2024

Rab37 mediates trafficking and membrane presentation of PD-1 to sustain T cell exhaustion in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wan-Ting Kuo,I-Ying Kuo,Hung-Chia Hsieh,Ssu-Ting Wu,Wu-Chou Su,Yi-Ching Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Plasma Membrane Protein Extraction Kit

Western blot - Plasma Membrane Protein Extraction Kit (AB65400)

Functional Studies - Plasma Membrane Protein Extraction Kit (AB65400)

Key facts

Assay time

Sample types

Complementary Products

Secondary Antibodies

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (223)

Product promise