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AB185906

Post-Bisulfite DNA Library Preparation Kit (For Illumina®)

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(1 Publication)

ab185906 is a complete set of optimized reagents to prepare a DNA library - after successful bisulfite conversion - for various Illumina® platform-based bisulfite sequencing (bisulfite-seq) assays, such as whole genome bisulfite sequencing (WGBS), oxidative bisulfite sequencing (oxBs-seq), reduced representative bisulfite sequencing (RRBS), and other bisulfite-based next generation sequencing applications.
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Spectrum - Post-Bisulfite DNA Library Preparation Kit (For Illumina®) (AB185906)
  • Spectrum

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Spectrum - Post-Bisulfite DNA Library Preparation Kit (For Illumina®) (AB185906)

Size distribution of library fragments as demonstrated by a post-bisulfite DNA library constructed using ab185906 Post-Bisulfite DNA Library Preparation Kit (For Illumina®) from 10 ng of input DNA.

Key facts

Assay time

5h

Range

0.5 - 1000 ng

Sensitivity

= 1 ng

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Product details

ab185906 is a complete set of optimized reagents to prepare a DNA library - after successful bisulfite conversion - for various Illumina® platform-based bisulfite sequencing (bisulfite-seq) assays, such as whole genome bisulfite sequencing (WGBS), oxidative bisulfite sequencing (oxBs-seq), reduced representative bisulfite sequencing (RRBS), and other bisulfite-based next generation sequencing applications. The optimized protocol and components of the kit allow both non-barcoded (singleplexed) and barcoded (multiplexed) DNA libraries to be quickly constructed using sub-nanogram input concentrations of DNA since the DNA is first bisulfite-converted and then used for library preparation.

Illumina® is a registered trademark of Illumina, Inc.

Several methods, including whole genome bisulfite sequencing (WGBS) and reduced representation bisulfite sequencing (RRBS), are currently used for genome-wide DNA methlyation analysis. These methods convert unmethylated cytosines to uracil while 5-methylcytosines remain unchanged by the bisulfite treatment. This allows epigenetic differences to become genetic differences, which can be subsequently detected via sequencing at the single-based resolution level and on a genome-wide scale. However, practical use with such current methods are not ideal as they (1) need large amounts of DNA (>1 μg) as input material, which is difficult to prepare from limited biological samples such as tumor biopsy, early embyros, embyronic tissues, and circulating DNA; (2) require DNA to first be sheared and then ligated to adapters, followed by bisulfite conversion (post-ligation bisulfite conversion), which causes substantial amounts of DNA fragments contained in the adapter-DNA fragment constructs to be broken and thereby forms mono-tagged templates that become removed during library enrichment; and (3) are time-consuming (2 days). The Post-Bisulfite DNA Library Preparation Kit (For Illumina®) is designed to overcome these weaknesses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2198:333-348 PubMed32822043

2020

Oxidative Bisulfite Sequencing: An Experimental and Computational Protocol.

Applications

Unspecified application

Species

Unspecified reactive species

Marie De Borre,Miguel R Branco
View all publications
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websiteProtocolBooklet
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