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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Abcam's Thrombin cleavage kit (ab207000) efficiently removes tags from recombinant fusion proteins containing an accessible thrombin cleavage sequence.
Application | Reactivity | Dilution info | Notes |
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Application Purification | Reactivity Reacts | Dilution info - | Notes - |
Abcam's Thrombin cleavage kit (ab207000) efficiently removes tags from recombinant fusion proteins containing an accessible thrombin cleavage sequence.
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Abcam's Thrombin cleavage kit (ab207000) efficiently removes tags from recombinant fusion proteins containing an accessible thrombin cleavage sequence.
Thrombin is a valuable biochemical tool due to its high proteolytic specificity. A thrombin cleavage site (e.g., Leu-Val-Pro-Arg-ll-Gly-Ser; where ll denotes the cleavage site) is widely incorporated within the linker region of fusion or affinity tagged recombinant proteins. After successful cleavage with thrombin, affinity tags or fused proteins can be separated from the target protein. Abcam's Thrombin cleavage kit provides an easy approach to test and optimize cleavage conditions of a target fusion or affinity-tagged protein containing a thrombin-specific cleavage site. The kit contains active thrombin enzyme sufficient to cleave up to 5 mg of the target protein. A 6x His-tagged protein containing the thrombin cleavage site is included as a cleavage control protein. Following cleavage of the target protein, thrombin can be removed by passing the reaction mix through a Heparin Sepharose® column.
This product is manufactured by BioVision, an Abcam company and was previously called K377 Thrombin Cleavage Kit. K377-500 is the same size as the 500 test size of ab207000.
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Terms & Conditions.
SDS-PAGE analysis of Cleavage Control Protein.
SDS-PAGE analysis of thrombin cleavage using different amount of thrombin and ab207000: Cleavage of 10 μg of 6x His-tagged Cleavage Control Protein with different amounts (0.01-1 U/μL) of thrombin at room temperature for 18 hours.
SDS-PAGE analysis of Cleavage Control Protein.
SDS-PAGE analysis of thrombin cleavage at different time points using ab207000: Cleavage of 10 μg of 6x His-tagged Cleavage Control Protein.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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