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Abcam's Thrombin cleavage kit (ab207000) efficiently removes tags from recombinant fusion proteins containing an accessible thrombin cleavage sequence.

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Reactivity data

Application

Purification

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

500 Test
Components
Active Thrombin
1 x 1 Vial
Thrombin Cleavage Buffer
1 x 25 mL
Thrombin Cleavage Control
1 x 1 Vial
Thrombin Dilution Buffer
1 x 1 mL

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Abcam's Thrombin cleavage kit (ab207000) efficiently removes tags from recombinant fusion proteins containing an accessible thrombin cleavage sequence.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Abcam's Thrombin cleavage kit (ab207000) efficiently removes tags from recombinant fusion proteins containing an accessible thrombin cleavage sequence.

Thrombin is a valuable biochemical tool due to its high proteolytic specificity. A thrombin cleavage site (e.g., Leu-Val-Pro-Arg-ll-Gly-Ser; where ll denotes the cleavage site) is widely incorporated within the linker region of fusion or affinity tagged recombinant proteins. After successful cleavage with thrombin, affinity tags or fused proteins can be separated from the target protein. Abcam's Thrombin cleavage kit provides an easy approach to test and optimize cleavage conditions of a target fusion or affinity-tagged protein containing a thrombin-specific cleavage site. The kit contains active thrombin enzyme sufficient to cleave up to 5 mg of the target protein. A 6x His-tagged protein containing the thrombin cleavage site is included as a cleavage control protein. Following cleavage of the target protein, thrombin can be removed by passing the reaction mix through a Heparin Sepharose® column.

This product is manufactured by BioVision, an Abcam company and was previously called K377 Thrombin Cleavage Kit. K377-500 is the same size as the 500 test size of ab207000.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Purification - Thrombin cleavage kit (ab207000), expandable thumbnail

    Purification - Thrombin cleavage kit (ab207000)

    SDS-PAGE analysis of Cleavage Control Protein.

    SDS-PAGE analysis of thrombin cleavage using different amount of thrombin and ab207000: Cleavage of 10 μg of 6x His-tagged Cleavage Control Protein with different amounts (0.01-1 U/μL) of thrombin at room temperature for 18 hours.

  • Purification - Thrombin cleavage kit (ab207000), expandable thumbnail

    Purification - Thrombin cleavage kit (ab207000)

    SDS-PAGE analysis of Cleavage Control Protein.

    SDS-PAGE analysis of thrombin cleavage at different time points using ab207000: Cleavage of 10 μg of 6x His-tagged Cleavage Control Protein.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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