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AB207000

Thrombin cleavage kit

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(2 Publications)

Thrombin cleavage kit (ab207000) efficiently removes tags (e.g. 6x His tag) from recombinant fusion proteins containing an accessible thrombin cleavage sequence.

- Suitable for recombinant protein purification and tag removal
- Contains sufficient active thrombin enzyme to cleave up to 5 mg of the target protein
- Kit includesThrombin cleavage control protein
2 Images
Purification - Thrombin cleavage kit (AB207000)
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Supplier Data

Purification - Thrombin cleavage kit (AB207000)

SDS-PAGE analysis of Cleavage Control Protein.

SDS-PAGE analysis of thrombin cleavage using different amount of thrombin and ab207000 : Cleavage of 10 μg of 6x His-tagged Cleavage Control Protein with different amounts (0.01-1 U/μL) of thrombin at room temperature for 18 hours.

Purification - Thrombin cleavage kit (AB207000)
  • P

Supplier Data

Purification - Thrombin cleavage kit (AB207000)

SDS-PAGE analysis of Cleavage Control Protein.

SDS-PAGE analysis of thrombin cleavage at different time points using ab207000 : Cleavage of 10 μg of 6x His-tagged Cleavage Control Protein.

Key facts

Sample types

Purified protein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Purification": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Thrombin cleavage kit (ab207000) efficiently removes tags from recombinant fusion proteins containing an accessible thrombin cleavage sequence.

Thrombin is a valuable biochemical tool due to its high proteolytic specificity. A thrombin cleavage site (e.g., Leu-Val-Pro-Arg-ll-Gly-Ser; where ll denotes the cleavage site) is widely incorporated within the linker region of fusion or affinity tagged recombinant proteins. After successful cleavage with thrombin, affinity tags or fused proteins can be separated from the target protein.

The ab207000 kit provides an easy approach to test and optimize cleavage conditions of a target fusion or affinity-tagged protein containing a thrombin-specific cleavage site.

Overview
The kit contains active thrombin enzyme sufficient to cleave up to 5 mg of the target protein.
A 6x His-tagged protein containing the thrombin cleavage site is included as a cleavage control protein.
Following cleavage of the target protein, thrombin can be removed by passing the reaction mix through a Heparin Sepharose® column.

This product is manufactured by BioVision, an Abcam company and was previously called K377 Thrombin Cleavage Kit. K377-500 is the same size as the 500 test size of ab207000.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "500Test": { "sellingSize": "500 Test", "publicAssetCode":"ab207000-500Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Thrombin Cleavage Control", "number":"AB207000-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"Thrombin Cleavage Buffer", "number":"AB207000-CMP02", "productcode":"" }, { "size":"1 x 10 µL", "name":"Active Thrombin", "number":"AB207000-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"Thrombin Dilution Buffer", "number":"AB207000-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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Publications (2)

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Product promise

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