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Whole Blood Polymorphonuclear Cell Isolation Kit (ab241001) is unique in the market because it enables the user to isolate Polymorphonuclear cells (PMNs), assess viability of the obtained cell fractions and also determine purity with three simple, easy-to-use reagents.

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Images

Schematic Diagram - Whole Blood Polymorphonuclear Cell Isolation Kit (AB241001), expandable thumbnail
  • Fluorescence Microscopy - Whole Blood Polymorphonuclear Cell Isolation Kit (AB241001), expandable thumbnail

Key facts

Sample type
Whole Blood

What's included?

30 Test
Components
Blunt-End Needle
15 x 1 Unit
Giemsa Stain
1 x 7 mL
PMN Density Gradient Solution
2 x 20 mL
PMN Isolation Buffer
2 x 30 mL
Viability Stain Mix
1 x 200 µL

Recommended products

Whole Blood Polymorphonuclear Cell Isolation Kit (ab241001) is unique in the market because it enables the user to isolate Polymorphonuclear cells (PMNs), assess viability of the obtained cell fractions and also determine purity with three simple, easy-to-use reagents.

Key facts

Sample type
Whole Blood

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Whole Blood Polymorphonuclear Cell Isolation Kit (ab241001) is unique in the market because it enables the user to isolate Polymorphonuclear cells (PMNs), assess viability of the obtained cell fractions and also determine purity with three simple, easy-to-use reagents. High yields, or 60-80% of total PMNs have been obtained yielding ≥ 5 x106 cells per mL of human whole blood. Purity of the cell fraction is assessed with giemsa stain (included). The kit's use results in highly enriched (>95%) cell fraction of polymorphonuclear cells with less than 3% red blood cell counts. More than 99% of the cells are viable, as determined by the Viability Stain.

30 test refers to 30 mL isolation.

This product is manufactured by BioVision, an Abcam company and was previously called K483 Human Whole Blood Polymorphonuclear Cell Isolation Kit. K483-30 is the same size as the 30 test size of ab241001.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Schematic Diagram - Whole Blood Polymorphonuclear Cell Isolation Kit (ab241001), expandable thumbnail

    Schematic Diagram - Whole Blood Polymorphonuclear Cell Isolation Kit (ab241001)

    Isolation Steps.

    A. Illustration of conical tube held at 45 degrees while blood layered on top of PMN DGM. B.1. Layers of PMN DGM and whole blood prior to and after centrifugation showing the separation of layers in the conical tube. B.2 Illustrates separation of six layers (plasma, PBMCs, PMN DGM, PMNs, PMN DGM and RBCs) obtained after spinning whole blood with PMN DGM.

  • Fluorescence Microscopy - Whole Blood Polymorphonuclear Cell Isolation Kit (ab241001), expandable thumbnail

    Fluorescence Microscopy - Whole Blood Polymorphonuclear Cell Isolation Kit (ab241001)

    Bright field and Fluorescent image.

    Bright-Field image of hemocytometer showing total cells(left); image from Fluorescent microscope with Rhodamine/FITC filters of same ROI showing live (green) and dead (red) cells (middle); merge of two panels (right). D. Polymorphonuclear Cells stained with Giemsa stain indicating purity of cell suspension obtained.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com