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AB241001

Whole Blood Polymorphonuclear Cell Isolation Kit

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Whole Blood Polymorphonuclear Cell Isolation Kit (ab241001) is used to isolate Polymorphonuclear cells (PMNs), assess viability of the obtained cell fractions and also determine sample purity with three simple, easy-to-use reagents. Isolated PMNs can be used in studies of production and release of cytokines/chemokines and are commonly used in migration assays

- Suitable to use for downstream application such as Flow cytometry or neutrophil chemotaxis assays
- All reagents are ready-to-use as supplied
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Fluorescence Microscopy - Whole Blood Polymorphonuclear Cell Isolation Kit (AB241001)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Whole Blood Polymorphonuclear Cell Isolation Kit (AB241001)

Bright field and Fluorescent image.

Bright-Field image of hemocytometer showing total cells(left); image from Fluorescent microscope with Rhodamine/FITC filters of same ROI showing live (green) and dead (red) cells (middle); merge of two panels (right). D. Polymorphonuclear Cells stained with Giemsa stain indicating purity of cell suspension obtained.

Schematic Diagram - Whole Blood Polymorphonuclear Cell Isolation Kit (AB241001)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Whole Blood Polymorphonuclear Cell Isolation Kit (AB241001)

Isolation Steps.

A. Illustration of conical tube held at 45 degrees while blood layered on top of PMN DGM. B.1. Layers of PMN DGM and whole blood prior to and after centrifugation showing the separation of layers in the conical tube. B.2 Illustrates separation of six layers (plasma, PBMCs, PMN DGM, PMNs, PMN DGM and RBCs) obtained after spinning whole blood with PMN DGM.

Key facts

Sample types

Whole Blood

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Product details

Whole Blood Polymorphonuclear Cell Isolation Kit (ab241001) is unique in the market because it enables the user to isolate Polymorphonuclear cells (PMNs), assess viability of the obtained cell fractions and also determine purity with three simple, easy-to-use reagents. High yields, or 60-80% of total PMNs have been obtained yielding ≥ 5 x106 cells per mL of human whole blood. Purity of the cell fraction is assessed with giemsa stain (included). The kit's use results in highly enriched (>95%) cell fraction of polymorphonuclear cells with less than 3% red blood cell counts. More than 99% of the cells are viable, as determined by the Viability Stain.

30 test refers to 30 mL isolation.

This product is manufactured by BioVision, an Abcam company and was previously called K483 Human Whole Blood Polymorphonuclear Cell Isolation Kit. K483-30 is the same size as the 30 test size of ab241001.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "30Test": { "sellingSize": "30 Test", "publicAssetCode":"ab241001-30Test", "assetComponentDetails": [ { "size":"15 x 1 Unit", "name":"Blunt-End Needle", "number":"AB241001-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"Viability Stain Mix", "number":"AB241001-CMP05", "productcode":"" }, { "size":"1 x 7 mL", "name":"Giemsa Stain", "number":"AB241001-CMP01", "productcode":"" }, { "size":"2 x 20 mL", "name":"PMN Density Gradient Solution", "number":"AB241001-CMP02", "productcode":"" }, { "size":"2 x 30 mL", "name":"PMN Isolation Buffer", "number":"AB241001-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com