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AB113475

Whole Cell Extraction Kit

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(6 Publications)

Abcam's Whole Cell Extraction Kit (ab113475) provides a simple and selective method for extracting whole cell proteins in just 45 minutes for a variety of applications such as western blotting, cellular enzyme assays, or any other procedures requiring cellular proteins.
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Functional Studies - Whole Cell Extraction Kit (AB113475)
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Supplier Data

Functional Studies - Whole Cell Extraction Kit (AB113475)

Whole cell extracts were prepared from MCF-7 cells using ab113475. Activity of SOD was measured using different amounts of the extract.

Key facts

Assay time

45m

Sample types

Suspension cells, Adherent cells

Reacts with

Mouse, Human

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Product details

Abcam's Whole Cell Extraction Kit (ab113475) provides a simple and selective method for extracting whole cell proteins in just 45 minutes for a variety of applications such as western blotting, cellular enzyme assays, or any other procedures requiring cellular proteins. ab113475 can be used to extract cellular proteins from mammalian cells and tissue samples.

Not sure if this is the right product for you? Check out our Methods and tools to study histone modifications for help.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab113475-100Test", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"1000X Protease Inhibitor Cocktail", "number":"AB113475-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"5X Extraction Buffer", "number":"AB113475-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"1000X DTT Solution", "number":"AB113475-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 30:457-474 PubMed36477078

2022

Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Xin Ji,Fei-Yan Zeng,Jian Zhou,Wen-Bin Wu,Xu-Jie Wang,Zhen Zhang,Guo-Yan Zhang,Jie Tong,Di-Yang Sun,Jia-Bao Zhang,Wen-Xiang Cao,Fu-Ming Shen,Jin-Jian Lu,Dong-Jie Li,Pei Wang

Pathogens (Basel, Switzerland) 10: PubMed34451426

2021

Alpha Enolase 1 Ubiquitination and Degradation Mediated by TRP120 Disrupts Glycolytic Flux and Promotes Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Zhu,Jere W McBride

Experimental and therapeutic medicine 16:985-992 PubMed30116349

2018

Metformin exerts anti-inflammatory effects on mouse colon smooth muscle cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Al-Dwairi,Mohammad Alqudah,Othman Al-Shboul,Mahmoud Alfaqih,Dana Alomari

FASEB journal : official publication of the Federa 32:5506-5519 PubMed29741927

2018

Transcellular transport of cobalamin in aortic endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Luciana Hannibal,Keerthana Bolisetty,Armend Axhemi,Patricia M DiBello,Edward V Quadros,Sergey Fedosov,Donald W Jacobsen

Biochemistry and biophysics reports 5:430-438 PubMed27047992

2016

Rapid identification of ubiquitination and SUMOylation target sites by microfluidic peptide array.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Zhu,Tierra R Farris,Sarah L Milligan,Haosi Chen,Ruijuan Zhu,Aailing Hong,Xiaochuan Zhou,Xiaolian Gao,Jere W McBride

Toxicology reports 2:322-332 PubMed28962365

2015

Upregulation of cyclin-dependent kinase inhibitors CDKN1B and CDKN1C in hepatocellular carcinoma-derived cells via goniothalamin-mediated protein stabilization and epigenetic modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ting Peng,Wen-Ren Wu,Lih-Ren Chen,Kung-Kai Kuo,Cheng-Hui Tsai,Yu-Ting Huang,Yu-Hsuan Lan,Fang-Rong Chang,Yang-Chang Wu,Yow-Ling Shiue
View all publications
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