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AB178779

Yeast Mitochondria Isolation Kit

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(7 Publications)

Yeast Mitochondria Isolation Kit (ab178779) is suitable to ssolate mitochondria are useful for studying mitochondrial respiration, assembly of respiratory complexes, apoptosis, mitochondrialDNA (mtDNA) and mtRNA and for protein profiling.

- Suitable for Pichia pastoris and Saccharomyces cerevisiae and other strains
- Compatible with fermentable media and non-fermentable media
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Functional Studies - Yeast Mitochondria Isolation Kit (AB178779)
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Supplier Data

Functional Studies - Yeast Mitochondria Isolation Kit (AB178779)

Mitochondrial integrity test.

Purified mitochondria were analyzed for intactness by using JC-1 dye, which tests the electrochemical proton gradient (ΔΨM) of the inner mitochondrial membrane. The intact purified mitochondria (control) show aggregation of JC-1 dye, whose signal can be measured at Ex/Em = 530/590 nm. Treatment with antimycin C (100 μM), an inhibitor of the electron transport chain, dissipates the mitochondrial membrane potential resulting in reduced fluorescence signal.

Key facts

Sample types

Suspension cells

Assay type

Direct

Reacts with

Pichia pastoris, Saccharomyces cerevisiae

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Product details

Isolated mitochondria are useful for studying mitochondrial respiration, assembly of respiratory complexes, apoptosis, mtDNA and mtRNA, and for protein profiling.

Abcam's Yeast Mitochondria Isolation Kit (ab178779) has been tested for Pichia pastoris and Saccharomyces cerevisiae and can be used to isolate mitochondria from other yeast.

Under fermentable media, the yield is ~ 150 - 200 μg of mitochondria, and under non-fermentable media (such as glycerol), # 200 - 250 μg of mitochondria from a culture growing at OD = 20.

ab178779 is only sufficient for 25 samples.

This product is manufactured by BioVision, an Abcam company and was previously called K259 Yeast Mitochondria Isolation Kit. K259-50 is the same size as the 50 test size of ab178779.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab178779-50Test", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Yeast Homogenization Buffer", "number":"AB178779-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"Dilution Buffer I", "number":"AB178779-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Protease Inhibitor Cocktail I", "number":"AB178779-CMP03", "productcode":"" }, { "size":"1 x 220 µL", "name":"Lysis Enzyme Mix", "number":"AB178779-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Buffer A", "number":"AB178779-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Buffer B", "number":"AB178779-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"DTT IV", "number":"AB178779-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:4151 PubMed40320395

2025

Design of diverse, functional mitochondrial targeting sequences across eukaryotic organisms using variational autoencoder.

Applications

Unspecified application

Species

Unspecified reactive species

Aashutosh Girish Boob,Shih-I Tan,Airah Zaidi,Nilmani Singh,Xueyi Xue,Shuaizhen Zhou,Teresa A Martin,Li-Qing Chen,Huimin Zhao

Redox biology 73:103174 PubMed38701646

2024

The absence of the ribosomal protein Rpl2702 elicits the MAPK-mTOR signaling to modulate mitochondrial morphology and functions.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Liu,Yifan Wu,Ke Liu,Mengdan Zhu,Shouhong Guang,Fengsong Wang,Xing Liu,Xuebiao Yao,Jiajia He,Chuanhai Fu

Journal of cell science 133: PubMed32265272

2020

Mitochondrial-nuclear heme trafficking in budding yeast is regulated by GTPases that control mitochondrial dynamics and ER contact sites.

Applications

Unspecified application

Species

Unspecified reactive species

Osiris Martinez-Guzman,Mathilda M Willoughby,Arushi Saini,Jonathan V Dietz,Iryna Bohovych,Amy E Medlock,Oleh Khalimonchuk,Amit R Reddi

eLife 9: PubMed31909711

2020

The genetic basis of aneuploidy tolerance in wild yeast.

Applications

Unspecified application

Species

Unspecified reactive species

James Hose,Leah E Escalante,Katie J Clowers,H Auguste Dutcher,DeElegant Robinson,Venera Bouriakov,Joshua J Coon,Evgenia Shishkova,Audrey P Gasch

Proceedings of the National Academy of Sciences of 116:16454-16462 PubMed31266891

2019

Meiotic viral attenuation through an ancestral apoptotic pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Gao,Sabrina Chau,Fuad Chowdhury,Tina Zhou,Saif Hossain,G Angus McQuibban,Marc D Meneghini

Genes & development 32:1309-1314 PubMed30228203

2018

From powerhouse to processing plant: conserved roles of mitochondrial outer membrane proteins in tRNA splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Wan,Anita K Hopper

Biochimica et biophysica acta. General subjects 1862:2545-2554 PubMed30056100

2018

Molecular mechanism of Arabidopsis thaliana profilins as antifungal proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Seong-Cheol Park,Il Ryong Kim,Jin-Young Kim,Yongjae Lee,Eun-Ji Kim,Ji Hyun Jung,Young Jun Jung,Mi-Kyeong Jang,Jung Ro Lee
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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