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Isolated mitochondria are useful for studying mitochondrial respiration, assembly of respiratory complexes, apoptosis, mtDNA and mtRNA, and for protein profiling.

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Functional Studies - Yeast Mitochondria Isolation Kit (AB178779), expandable thumbnail

Publications

Key facts

Sample type
Suspension cells
Assay type
Direct
Reactive species
Pichia pastoris, Saccharomyces cerevisiae

What's included?

50 Test
Components
Buffer A
1 x 50 mL
Buffer B
1 x 50 mL
DTT IV
1 x 1 mL
Dilution Buffer I
1 x 10 mL
Lysis Enzyme Mix
1 x 220 µL
Protease Inhibitor Cocktail I
1 x 1 Vial
Yeast Homogenization Buffer
1 x 50 mL

Recommended products

Isolated mitochondria are useful for studying mitochondrial respiration, assembly of respiratory complexes, apoptosis, mtDNA and mtRNA, and for protein profiling.

Key facts

Sample type
Suspension cells
Assay type
Direct
Reactive species
Pichia pastoris, Saccharomyces cerevisiae

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Isolated mitochondria are useful for studying mitochondrial respiration, assembly of respiratory complexes, apoptosis, mtDNA and mtRNA, and for protein profiling.

Abcam's Yeast Mitochondria Isolation Kit (ab178779) has been tested for Pichia pastoris and Saccharomyces cerevisiae and can be used to isolate mitochondria from other yeast.

Under fermentable media, the yield is ~ 150 - 200 μg of mitochondria, and under non-fermentable media (such as glycerol), # 200 - 250 μg of mitochondria from a culture growing at OD = 20.

ab178779 is only sufficient for 25 samples.

This product is manufactured by BioVision, an Abcam company and was previously called K259 Yeast Mitochondria Isolation Kit. K259-50 is the same size as the 50 test size of ab178779.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Functional Studies - Yeast Mitochondria Isolation Kit (ab178779), expandable thumbnail

    Functional Studies - Yeast Mitochondria Isolation Kit (ab178779)

    Mitochondrial integrity test.

    Purified mitochondria were analyzed for intactness by using JC-1 dye, which tests the electrochemical proton gradient (ΔΨM) of the inner mitochondrial membrane. The intact purified mitochondria (control) show aggregation of JC-1 dye, whose signal can be measured at Ex/Em = 530/590 nm. Treatment with antimycin C (100 μM), an inhibitor of the electron transport chain, dissipates the mitochondrial membrane potential resulting in reduced fluorescence signal.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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