Secondary antibodies
Choose the right antibody for you from our broad range of conjugated secondary antibodies.
Fluorescent secondary antibodies
Explore our fluorescent secondary antibodies to expand primary antibody detection capabilities, improving visualization in various assays.
Other secondary antibodies
Explore a versatile range of secondary antibodies to enhance your research accuracy and efficiency.
Secondary antibodies indirectly detect a primary antibody that specifically binds to the protein of interest. They're made by immunizing a host animal with antibodies from a different species. Immunizing a goat with rabbit IgG will generate goat anti-rabbit IgG secondary antibodies. The secondary antibody selected should match the species in which the primary antibody was raised in, as well as the isotype of the primary antibody of interest. For example, a goat anti-rabbit IgG secondary antibody can be used to detect the signal of rabbit IgG primary antibodies.
Secondary antibodies can be conjugated to fluorophores, such as Alexa Fluor® dyes. Our catalog has secondary antibodies conjugated to nine Alexa Fluor® dyes, which are ideal for multi-color analysis and use in cell imaging and flow cytometry experiments. Learn about Alexa Fluor® conjugated secondary antibodies at Abcam here. Explore our best-selling Alexa Fluor® secondary antibody.
Secondary antibodies can also be conjugated to enzymes, like HRP and alkaline phosphatase, or proteins like biotin, to amplify the signal of primary antibodies. We offer HRP-conjugated secondary antibodies against diverse species and their isotypes, as well as in fragment and pre-adsorbed forms. Click here to learn more about out HRP offering. We also offer colloidal gold conjugated secondary antibodies that are suitable for electron microscopy (EM) assays. See our range here.