Secondary antibodies indirectly detect a primary antibody that specifically binds to the protein of interest. Secondary antibodies are crucial tools for amplifying protein target signals and enhancing visual detection in various immunoassays. Choose from our extensive range to match your specific research needs.

Secondary antibodies are essential if you're aiming to detect, analyze, or quantify target antigens using less direct methods than primary antibody binding. They're made by immunizing a host animal with antibodies from a different species. Immunizing a goat with rabbit IgG will generate goat anti-rabbit IgG secondary antibodies. The secondary antibody selected should match the species in which the primary antibody was raised in, as well as the isotype of the primary antibody of interest.

After binding to the primary antibody, the secondary antibody is visualized through various labeling techniques, such as fluorescent dyes like Alexa Fluor® or enzymes like horseradish peroxidase (HRP). We offer a broad selection of secondary antibodies designed to optimize your experimental outcomes. Whether you’re conducting fluorescence-based cell imaging, flow cytometry, or more traditional colorimetric assays like western blotting, our secondary antibodies provide high specificity, minimal cross-reactivity, and robust performance. Our range includes HRP-conjugated, Alexa Fluor®-conjugated, and other fluorescently labeled antibodies,  tailored to meet the demands of today's complex, multi-color experiments. By choosing our secondary antibodies, you can get products that enhance signal detection and data clarity, moving your research forward.

 
HRP conjugated secondary antibodies

Horseradish peroxidase (HRP) conjugated secondary antibodies facilitate colorimetric detection in immunohistochemistry and western blotting with high signal amplification.

 
Alexa Fluor® secondary antibodies

Labeled with highly fluorescent Alexa Fluor® dyes, these antibodies are perfect for vibrant, multi-color fluorescent imaging applications. Our catalog features secondary antibodies conjugated to nine Alexa Fluor® dyes, including Alexa Fluor® 405, 488, 555, 568, 594, 647, 680, 750, 790.

Fluorescent secondary antibodies

These antibodies are conjugated with a variety of fluorescent dyes other than Alexa Fluor®, and are suitable for various fluorescence-based detection methods.