Alpaca monoclonal [10A4] Anti-Mouse IgG1 antibody (ATTO 488)

Product details

Together with their monovalent binding mode, the defined fluorophore conjugation of ab325288 results in a quantitative and reliable readout for several fluorescence-based applications.  It contains a defined number of fluorophores at known positions. In addition due to the small size the fluorophore displacement from the bound target protein is very low making ab325288 ideal for standard super resolution technologies.

Features:
- High affinity monovalent binding + two fluorophores per sdAb for brighter signals.
- Reagent is made from small-sized proteins. This ensures optimal access to the respective target protein, also in crowded environment or 3D samples.
In comparison to conventional secondary reagents the ab325288 nanobody has the following advantages:
- Small probes for enhanced staining speed and improved tissue penetration and higher resolution.
- Monoclonal tools with defined species  and isotype specificity.
- Recombinant production for reliable quality, scalability and reproducibility.
- Monovalent binding mode – Pre-incubation without clustering primary antibodies and therefore one-step indirect immunofluorescence possible.
- Enhanced binding allows one-step multi-colour staining and imaging even with primary antibodies from identical species and isotype (Multiplexing).
- Suitable for post-fixation and enhanced performance in demanding application (e.g., expansion microscopy, DNA-PAINT, dSTORM, etc.).