Alpaca monoclonal [14A4] Anti-Mouse IgG2a/b antibody (ATTO 488) - FluoTag®-X2

Product details

FluoTags®

FluoTags are fully defined & ready-to-go fluorescent camelid single-domain antibody (sdAb) conjugates. All FluoTags® carry a defined number of fluorophores at known positions. Accordingly, they are available as FluoTag®-X2 or FluoTag®X4.

Together with their monovalent binding mode, the defined fluorophore conjugation of FluoTags® results in a more quantitative and reliable readout in all fluorescence-based analyses. Due to the small size the fluorophore displacement from the bound target protein is very low and therefore FluoTags® are ideal for standard super resolution technologies.

FluoTag®-X2 (One target - One sdAb – Two fluorophores (1:1:2)

FluoTag®-X2 reagents are composed of a single sdAb carrying two fluorophores per protein molecule. The maximal displacement of fluorophores from the antigen-binding surface is ~3 nm. FluoTag®-X2 reagents show good tissue penetration features.

Features:

• High affinity monovalent binding + two fluorophores per sdAb for brighter signals
• Reagent is made from small-sized proteins. This ensures optimal access to the respective target protein, also in crowded environment or 3D samples.

Smart Secondaries®

Smart Secondaries® are a unique set of high-affinity single-domain antibodies recognizing conventional immunoglobulins with exquisite species and isotype specificity. They provide unique benefits for all antibody-based assays in research and diagnostics by combining reproducible specificity and production with small and high-affinity monovalent binding. The technology offers surprising new possibilities like one-step indirect immunofluorescence or easy multiplexing.

In comparison to conventional secondary reagents, Smart Secondary® nanobodies feature significant advantages:

1. Small probes for enhanced staining speed and improved tissue penetration and higher resolution.
2. Monoclonal tools: Clearly defined species specificity – once and forever!
3. Isotype specificity for independent detection of mouse IgG1 and IgG2a/b isotypes.
4. Recombinant production for reliable quality, scalability and reproducibility without using animals.
5. Monovalent binding mode – Pre-incubation without clustering primary antibodies and therefore one-step indirect immunofluorescence possible!
6. Extraordinarily strong binding allows one-step multi-colour staining and imaging even with primary antibodies from identical species and isotype (Multiplexing)
7. Equipped with propriety FX technology for improved post-fixation and enhanced performance in demanding application (e.g., expansion microscopy, DNA-PAINT, dSTORM, etc.